Thalassobacter sp. 16PALIMAR09

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Thalassobacter; unclassified Thalassobacter

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3345 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A099T7U0|A0A099T7U0_9RHOB Methylhydantoinase OS=Thalassobacter sp. 16PALIMAR09 OX=1225651 GN=PM04_14600 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.19KK3 pKa = 10.41VLFATTAIVAFAGAASADD21 pKa = 3.79VAVSGSAEE29 pKa = 4.56LGIADD34 pKa = 4.55NGTATEE40 pKa = 4.11NVGFFSSVDD49 pKa = 3.17VRR51 pKa = 11.84FSMTGEE57 pKa = 4.07SDD59 pKa = 2.9NGLAFGATIDD69 pKa = 5.17LDD71 pKa = 4.07DD72 pKa = 4.73ALEE75 pKa = 4.47AGGNAAAQGNLADD88 pKa = 4.61AGQGIGDD95 pKa = 3.88NSDD98 pKa = 3.5NDD100 pKa = 4.44SIDD103 pKa = 3.19YY104 pKa = 9.39TIFVSGAFGSLTFGDD119 pKa = 3.87TDD121 pKa = 4.46GAMDD125 pKa = 3.67WALTEE130 pKa = 4.46GGNVGNPGSINDD142 pKa = 4.43AEE144 pKa = 4.28TSYY147 pKa = 11.45AGYY150 pKa = 10.75AGAYY154 pKa = 10.08LDD156 pKa = 4.33GNGDD160 pKa = 3.81GMIARR165 pKa = 11.84YY166 pKa = 9.84DD167 pKa = 3.76YY168 pKa = 11.89AFGDD172 pKa = 3.71YY173 pKa = 10.71ALAVSVEE180 pKa = 3.92QGVLDD185 pKa = 4.3EE186 pKa = 4.68EE187 pKa = 4.05NGYY190 pKa = 11.08AIGFKK195 pKa = 10.92GNVAGVSFGIAHH207 pKa = 5.94QEE209 pKa = 3.92GTAGAVGGANADD221 pKa = 3.63LTGVSLGYY229 pKa = 11.06GFGDD233 pKa = 4.1LKK235 pKa = 11.07AGIMLATGEE244 pKa = 4.16NRR246 pKa = 11.84LGQDD250 pKa = 3.9DD251 pKa = 5.84DD252 pKa = 5.27LMQLGLGYY260 pKa = 7.13TTGAVSLHH268 pKa = 5.99ANYY271 pKa = 10.77AEE273 pKa = 4.21RR274 pKa = 11.84EE275 pKa = 4.37TNNVQSDD282 pKa = 4.24GFGVAAKK289 pKa = 10.26YY290 pKa = 10.6DD291 pKa = 3.8LGGGAGVHH299 pKa = 5.2VAYY302 pKa = 10.37GSGSVGTAASVDD314 pKa = 3.42RR315 pKa = 11.84WSLGLAMSFF324 pKa = 3.97

Molecular weight: 32.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A099T7K9|A0A099T7K9_9RHOB Isomerase OS=Thalassobacter sp. 16PALIMAR09 OX=1225651 GN=PM04_10385 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3345

0

3345

1055750

39

18588

315.6

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.547 ± 0.064

0.894 ± 0.021

6.039 ± 0.067

5.666 ± 0.046

3.673 ± 0.032

8.631 ± 0.071

2.003 ± 0.032

5.222 ± 0.027

3.113 ± 0.045

9.817 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.837 ± 0.038

2.716 ± 0.043

5.034 ± 0.067

3.329 ± 0.023

6.212 ± 0.068

5.265 ± 0.062

6.019 ± 0.093

7.47 ± 0.043

1.36 ± 0.021

2.155 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski