Kitasatospora sp. Root187

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Kitasatospora; unclassified Kitasatospora

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6308 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8NQC9|A0A0Q8NQC9_9ACTN Uncharacterized protein OS=Kitasatospora sp. Root187 OX=1736486 GN=ASE03_08735 PE=4 SV=1
MM1 pKa = 7.27TGEE4 pKa = 4.2ATTGDD9 pKa = 4.11ALEE12 pKa = 4.08VWIDD16 pKa = 3.4QDD18 pKa = 3.99LCTGDD23 pKa = 4.65GICVQYY29 pKa = 10.96APEE32 pKa = 4.18VFEE35 pKa = 5.9LDD37 pKa = 3.05IDD39 pKa = 3.65GLAYY43 pKa = 10.76VKK45 pKa = 10.94GDD47 pKa = 3.77DD48 pKa = 5.07DD49 pKa = 5.1EE50 pKa = 6.06LRR52 pKa = 11.84QQPGDD57 pKa = 3.85SAPVPLTLLQDD68 pKa = 3.88VVDD71 pKa = 4.41SAKK74 pKa = 10.25EE75 pKa = 3.92CPGDD79 pKa = 4.18CIHH82 pKa = 6.36VRR84 pKa = 11.84RR85 pKa = 11.84VSDD88 pKa = 3.3RR89 pKa = 11.84VEE91 pKa = 4.23VYY93 pKa = 10.89GPDD96 pKa = 3.33ADD98 pKa = 3.69

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8NUL0|A0A0Q8NUL0_9ACTN Fur family transcriptional regulator OS=Kitasatospora sp. Root187 OX=1736486 GN=ASE03_06885 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.84GRR40 pKa = 11.84TNISAA45 pKa = 3.72

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6308

0

6308

2030878

29

5184

322.0

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.756 ± 0.043

0.775 ± 0.009

5.616 ± 0.024

5.51 ± 0.035

2.751 ± 0.019

9.39 ± 0.028

2.18 ± 0.017

3.15 ± 0.02

2.008 ± 0.025

11.059 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.603 ± 0.012

1.892 ± 0.023

6.154 ± 0.03

3.144 ± 0.02

7.595 ± 0.04

5.133 ± 0.022

6.258 ± 0.029

8.318 ± 0.031

1.573 ± 0.014

2.129 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski