Amino acid dipepetide frequency for Kitasatospora sp. Root187

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.459AlaAla: 22.459 ± 0.169	1.033AlaCys: 1.033 ± 0.023	8.534AlaAsp: 8.534 ± 0.078	9.612AlaGlu: 9.612 ± 0.11	3.561AlaPhe: 3.561 ± 0.039	13.378AlaGly: 13.378 ± 0.084	2.55AlaHis: 2.55 ± 0.042	3.63AlaIle: 3.63 ± 0.046	2.983AlaLys: 2.983 ± 0.05	15.011AlaLeu: 15.011 ± 0.128	2.366AlaMet: 2.366 ± 0.04	2.235AlaAsn: 2.235 ± 0.045	7.068AlaPro: 7.068 ± 0.095	3.931AlaGln: 3.931 ± 0.052	9.27AlaArg: 9.27 ± 0.081	5.485AlaSer: 5.485 ± 0.057	7.488AlaThr: 7.488 ± 0.078	12.509AlaVal: 12.509 ± 0.083	1.812AlaTrp: 1.812 ± 0.033	2.643AlaTyr: 2.643 ± 0.037	0.006AlaXaa: 0.006 ± 0.002
Cys	1.022CysAla: 1.022 ± 0.025	0.09CysCys: 0.09 ± 0.007	0.435CysAsp: 0.435 ± 0.014	0.364CysGlu: 0.364 ± 0.013	0.237CysPhe: 0.237 ± 0.012	0.926CysGly: 0.926 ± 0.02	0.178CysHis: 0.178 ± 0.01	0.171CysIle: 0.171 ± 0.009	0.09CysLys: 0.09 ± 0.007	0.816CysLeu: 0.816 ± 0.023	0.128CysMet: 0.128 ± 0.008	0.146CysAsn: 0.146 ± 0.009	0.49CysPro: 0.49 ± 0.018	0.188CysGln: 0.188 ± 0.01	0.578CysArg: 0.578 ± 0.019	0.484CysSer: 0.484 ± 0.019	0.546CysThr: 0.546 ± 0.015	0.547CysVal: 0.547 ± 0.016	0.154CysTrp: 0.154 ± 0.007	0.163CysTyr: 0.163 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.643AspAla: 6.643 ± 0.07	0.445AspCys: 0.445 ± 0.016	2.873AspAsp: 2.873 ± 0.046	3.286AspGlu: 3.286 ± 0.051	1.629AspPhe: 1.629 ± 0.03	6.173AspGly: 6.173 ± 0.066	1.374AspHis: 1.374 ± 0.027	1.604AspIle: 1.604 ± 0.033	0.978AspLys: 0.978 ± 0.026	6.369AspLeu: 6.369 ± 0.055	0.614AspMet: 0.614 ± 0.016	0.972AspAsn: 0.972 ± 0.027	4.586AspPro: 4.586 ± 0.05	1.996AspGln: 1.996 ± 0.036	4.804AspArg: 4.804 ± 0.052	2.634AspSer: 2.634 ± 0.038	2.962AspThr: 2.962 ± 0.043	3.914AspVal: 3.914 ± 0.046	1.13AspTrp: 1.13 ± 0.027	1.167AspTyr: 1.167 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	6.889GluAla: 6.889 ± 0.082	0.333GluCys: 0.333 ± 0.013	2.463GluAsp: 2.463 ± 0.036	2.971GluGlu: 2.971 ± 0.05	1.553GluPhe: 1.553 ± 0.03	3.731GluGly: 3.731 ± 0.05	1.519GluHis: 1.519 ± 0.031	2.233GluIle: 2.233 ± 0.035	1.131GluLys: 1.131 ± 0.029	8.036GluLeu: 8.036 ± 0.081	0.775GluMet: 0.775 ± 0.02	0.943GluAsn: 0.943 ± 0.019	3.193GluPro: 3.193 ± 0.043	2.824GluGln: 2.824 ± 0.044	4.968GluArg: 4.968 ± 0.062	2.461GluSer: 2.461 ± 0.036	2.664GluThr: 2.664 ± 0.037	4.469GluVal: 4.469 ± 0.055	0.742GluTrp: 0.742 ± 0.019	1.204GluTyr: 1.204 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.758PheAla: 3.758 ± 0.041	0.267PheCys: 0.267 ± 0.012	1.923PheAsp: 1.923 ± 0.03	1.499PheGlu: 1.499 ± 0.028	0.845PhePhe: 0.845 ± 0.024	3.14PheGly: 3.14 ± 0.043	0.599PheHis: 0.599 ± 0.019	0.688PheIle: 0.688 ± 0.016	0.522PheLys: 0.522 ± 0.018	2.59PheLeu: 2.59 ± 0.04	0.372PheMet: 0.372 ± 0.014	0.639PheAsn: 0.639 ± 0.015	1.372PhePro: 1.372 ± 0.029	0.774PheGln: 0.774 ± 0.019	1.859PheArg: 1.859 ± 0.033	1.455PheSer: 1.455 ± 0.026	2.049PheThr: 2.049 ± 0.032	2.131PheVal: 2.131 ± 0.038	0.436PheTrp: 0.436 ± 0.014	0.591PheTyr: 0.591 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	10.886GlyAla: 10.886 ± 0.08	0.825GlyCys: 0.825 ± 0.023	4.586GlyAsp: 4.586 ± 0.052	4.858GlyGlu: 4.858 ± 0.055	2.873GlyPhe: 2.873 ± 0.041	8.515GlyGly: 8.515 ± 0.094	2.202GlyHis: 2.202 ± 0.037	3.362GlyIle: 3.362 ± 0.043	2.328GlyLys: 2.328 ± 0.039	10.347GlyLeu: 10.347 ± 0.093	1.882GlyMet: 1.882 ± 0.036	1.871GlyAsn: 1.871 ± 0.043	5.248GlyPro: 5.248 ± 0.056	3.142GlyGln: 3.142 ± 0.044	7.48GlyArg: 7.48 ± 0.072	5.668GlySer: 5.668 ± 0.06	6.212GlyThr: 6.212 ± 0.072	7.274GlyVal: 7.274 ± 0.07	1.916GlyTrp: 1.916 ± 0.034	2.421GlyTyr: 2.421 ± 0.038	0.002GlyXaa: 0.002 ± 0.001
His	2.417HisAla: 2.417 ± 0.039	0.221HisCys: 0.221 ± 0.01	1.163HisAsp: 1.163 ± 0.025	1.028HisGlu: 1.028 ± 0.025	0.638HisPhe: 0.638 ± 0.018	2.278HisGly: 2.278 ± 0.029	0.647HisHis: 0.647 ± 0.02	0.528HisIle: 0.528 ± 0.018	0.298HisLys: 0.298 ± 0.012	2.549HisLeu: 2.549 ± 0.035	0.286HisMet: 0.286 ± 0.01	0.39HisAsn: 0.39 ± 0.014	1.826HisPro: 1.826 ± 0.029	0.779HisGln: 0.779 ± 0.018	2.153HisArg: 2.153 ± 0.034	1.062HisSer: 1.062 ± 0.023	1.235HisThr: 1.235 ± 0.022	1.435HisVal: 1.435 ± 0.029	0.398HisTrp: 0.398 ± 0.013	0.47HisTyr: 0.47 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.788IleAla: 4.788 ± 0.054	0.297IleCys: 0.297 ± 0.011	2.148IleAsp: 2.148 ± 0.037	1.943IleGlu: 1.943 ± 0.036	0.736IlePhe: 0.736 ± 0.02	3.698IleGly: 3.698 ± 0.054	0.574IleHis: 0.574 ± 0.016	0.857IleIle: 0.857 ± 0.023	0.711IleLys: 0.711 ± 0.021	2.292IleLeu: 2.292 ± 0.039	0.393IleMet: 0.393 ± 0.012	0.751IleAsn: 0.751 ± 0.021	1.818IlePro: 1.818 ± 0.033	0.793IleGln: 0.793 ± 0.02	2.266IleArg: 2.266 ± 0.035	1.809IleSer: 1.809 ± 0.028	2.258IleThr: 2.258 ± 0.034	2.415IleVal: 2.415 ± 0.036	0.425IleTrp: 0.425 ± 0.016	0.527IleTyr: 0.527 ± 0.015	0.001IleXaa: 0.001 ± 0.001
Lys	2.808LysAla: 2.808 ± 0.048	0.111LysCys: 0.111 ± 0.008	1.126LysAsp: 1.126 ± 0.027	0.97LysGlu: 0.97 ± 0.024	0.481LysPhe: 0.481 ± 0.015	1.612LysGly: 1.612 ± 0.031	0.431LysHis: 0.431 ± 0.014	0.828LysIle: 0.828 ± 0.023	0.628LysLys: 0.628 ± 0.021	2.11LysLeu: 2.11 ± 0.034	0.338LysMet: 0.338 ± 0.012	0.489LysAsn: 0.489 ± 0.017	1.274LysPro: 1.274 ± 0.027	0.718LysGln: 0.718 ± 0.02	1.221LysArg: 1.221 ± 0.025	1.116LysSer: 1.116 ± 0.027	1.182LysThr: 1.182 ± 0.029	1.906LysVal: 1.906 ± 0.031	0.242LysTrp: 0.242 ± 0.011	0.492LysTyr: 0.492 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	17.169LeuAla: 17.169 ± 0.123	0.813LeuCys: 0.813 ± 0.02	6.774LeuAsp: 6.774 ± 0.066	4.698LeuGlu: 4.698 ± 0.052	2.67LeuPhe: 2.67 ± 0.046	10.124LeuGly: 10.124 ± 0.089	2.408LeuHis: 2.408 ± 0.036	3.484LeuIle: 3.484 ± 0.049	1.892LeuLys: 1.892 ± 0.03	12.866LeuLeu: 12.866 ± 0.132	1.572LeuMet: 1.572 ± 0.031	1.891LeuAsn: 1.891 ± 0.036	7.202LeuPro: 7.202 ± 0.07	2.373LeuGln: 2.373 ± 0.039	8.841LeuArg: 8.841 ± 0.082	5.448LeuSer: 5.448 ± 0.054	7.866LeuThr: 7.866 ± 0.062	9.259LeuVal: 9.259 ± 0.084	1.34LeuTrp: 1.34 ± 0.027	1.899LeuTyr: 1.899 ± 0.037	0.002LeuXaa: 0.002 ± 0.001
Met	2.138MetAla: 2.138 ± 0.035	0.112MetCys: 0.112 ± 0.007	0.783MetAsp: 0.783 ± 0.019	0.62MetGlu: 0.62 ± 0.018	0.434MetPhe: 0.434 ± 0.014	1.127MetGly: 1.127 ± 0.025	0.326MetHis: 0.326 ± 0.012	0.638MetIle: 0.638 ± 0.016	0.369MetLys: 0.369 ± 0.014	1.71MetLeu: 1.71 ± 0.032	0.267MetMet: 0.267 ± 0.013	0.397MetAsn: 0.397 ± 0.014	1.052MetPro: 1.052 ± 0.024	0.439MetGln: 0.439 ± 0.014	1.192MetArg: 1.192 ± 0.021	1.176MetSer: 1.176 ± 0.026	1.49MetThr: 1.49 ± 0.026	1.286MetVal: 1.286 ± 0.025	0.186MetTrp: 0.186 ± 0.011	0.287MetTyr: 0.287 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.23AsnAla: 2.23 ± 0.043	0.181AsnCys: 0.181 ± 0.01	0.986AsnAsp: 0.986 ± 0.024	0.85AsnGlu: 0.85 ± 0.024	0.535AsnPhe: 0.535 ± 0.018	2.188AsnGly: 2.188 ± 0.045	0.403AsnHis: 0.403 ± 0.014	0.63AsnIle: 0.63 ± 0.019	0.377AsnLys: 0.377 ± 0.013	1.868AsnLeu: 1.868 ± 0.032	0.305AsnMet: 0.305 ± 0.013	0.491AsnAsn: 0.491 ± 0.019	1.491AsnPro: 1.491 ± 0.033	0.624AsnGln: 0.624 ± 0.019	1.322AsnArg: 1.322 ± 0.027	1.149AsnSer: 1.149 ± 0.03	1.164AsnThr: 1.164 ± 0.033	1.357AsnVal: 1.357 ± 0.032	0.335AsnTrp: 0.335 ± 0.015	0.438AsnTyr: 0.438 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	9.246ProAla: 9.246 ± 0.089	0.319ProCys: 0.319 ± 0.013	4.294ProAsp: 4.294 ± 0.05	4.352ProGlu: 4.352 ± 0.053	1.546ProPhe: 1.546 ± 0.029	6.705ProGly: 6.705 ± 0.069	1.179ProHis: 1.179 ± 0.026	1.42ProIle: 1.42 ± 0.027	1.179ProLys: 1.179 ± 0.025	5.538ProLeu: 5.538 ± 0.059	0.953ProMet: 0.953 ± 0.02	1.071ProAsn: 1.071 ± 0.026	3.318ProPro: 3.318 ± 0.058	1.886ProGln: 1.886 ± 0.042	3.419ProArg: 3.419 ± 0.05	3.249ProSer: 3.249 ± 0.054	3.729ProThr: 3.729 ± 0.045	5.794ProVal: 5.794 ± 0.062	0.9ProTrp: 0.9 ± 0.025	1.438ProTyr: 1.438 ± 0.027	0.002ProXaa: 0.002 ± 0.001
Gln	4.261GlnAla: 4.261 ± 0.051	0.189GlnCys: 0.189 ± 0.009	1.528GlnAsp: 1.528 ± 0.027	1.456GlnGlu: 1.456 ± 0.027	0.76GlnPhe: 0.76 ± 0.019	2.448GlnGly: 2.448 ± 0.037	0.746GlnHis: 0.746 ± 0.019	1.181GlnIle: 1.181 ± 0.023	0.552GlnLys: 0.552 ± 0.019	4.122GlnLeu: 4.122 ± 0.045	0.441GlnMet: 0.441 ± 0.017	0.565GlnAsn: 0.565 ± 0.016	2.033GlnPro: 2.033 ± 0.039	1.714GlnGln: 1.714 ± 0.043	2.508GlnArg: 2.508 ± 0.038	1.476GlnSer: 1.476 ± 0.03	1.453GlnThr: 1.453 ± 0.032	2.761GlnVal: 2.761 ± 0.042	0.526GlnTrp: 0.526 ± 0.019	0.724GlnTyr: 0.724 ± 0.02	0.001GlnXaa: 0.001 ± 0.001
Arg	9.356ArgAla: 9.356 ± 0.081	0.57ArgCys: 0.57 ± 0.016	3.585ArgAsp: 3.585 ± 0.038	4.394ArgGlu: 4.394 ± 0.061	2.211ArgPhe: 2.211 ± 0.034	4.997ArgGly: 4.997 ± 0.057	1.9ArgHis: 1.9 ± 0.036	3.214ArgIle: 3.214 ± 0.048	1.467ArgLys: 1.467 ± 0.027	8.929ArgLeu: 8.929 ± 0.085	1.597ArgMet: 1.597 ± 0.028	1.355ArgAsn: 1.355 ± 0.025	4.931ArgPro: 4.931 ± 0.061	2.621ArgGln: 2.621 ± 0.04	7.105ArgArg: 7.105 ± 0.085	4.214ArgSer: 4.214 ± 0.049	5.067ArgThr: 5.067 ± 0.045	5.198ArgVal: 5.198 ± 0.051	1.397ArgTrp: 1.397 ± 0.029	1.844ArgTyr: 1.844 ± 0.034	0.003ArgXaa: 0.003 ± 0.001
Ser	6.955SerAla: 6.955 ± 0.066	0.473SerCys: 0.473 ± 0.016	2.454SerAsp: 2.454 ± 0.036	2.286SerGlu: 2.286 ± 0.035	1.625SerPhe: 1.625 ± 0.027	5.871SerGly: 5.871 ± 0.061	0.992SerHis: 0.992 ± 0.023	1.525SerIle: 1.525 ± 0.029	1.013SerLys: 1.013 ± 0.023	4.816SerLeu: 4.816 ± 0.052	1.064SerMet: 1.064 ± 0.021	0.987SerAsn: 0.987 ± 0.029	3.251SerPro: 3.251 ± 0.044	1.324SerGln: 1.324 ± 0.028	3.503SerArg: 3.503 ± 0.043	3.106SerSer: 3.106 ± 0.054	3.4SerThr: 3.4 ± 0.049	4.244SerVal: 4.244 ± 0.045	1.124SerTrp: 1.124 ± 0.024	1.313SerTyr: 1.313 ± 0.026	0.001SerXaa: 0.001 ± 0.001
Thr	9.443ThrAla: 9.443 ± 0.087	0.414ThrCys: 0.414 ± 0.015	3.667ThrAsp: 3.667 ± 0.047	3.427ThrGlu: 3.427 ± 0.043	1.681ThrPhe: 1.681 ± 0.029	6.938ThrGly: 6.938 ± 0.063	1.121ThrHis: 1.121 ± 0.025	1.675ThrIle: 1.675 ± 0.034	1.17ThrLys: 1.17 ± 0.029	6.035ThrLeu: 6.035 ± 0.046	0.923ThrMet: 0.923 ± 0.022	1.089ThrAsn: 1.089 ± 0.033	4.3ThrPro: 4.3 ± 0.053	1.336ThrGln: 1.336 ± 0.027	3.665ThrArg: 3.665 ± 0.048	3.046ThrSer: 3.046 ± 0.047	3.989ThrThr: 3.989 ± 0.067	6.564ThrVal: 6.564 ± 0.062	0.88ThrTrp: 0.88 ± 0.02	1.215ThrTyr: 1.215 ± 0.033	0.001ThrXaa: 0.001 ± 0.001
Val	10.622ValAla: 10.622 ± 0.087	0.7ValCys: 0.7 ± 0.02	4.654ValAsp: 4.654 ± 0.055	4.684ValGlu: 4.684 ± 0.058	2.314ValPhe: 2.314 ± 0.036	6.674ValGly: 6.674 ± 0.063	1.829ValHis: 1.829 ± 0.032	2.858ValIle: 2.858 ± 0.039	1.705ValLys: 1.705 ± 0.036	10.242ValLeu: 10.242 ± 0.086	1.284ValMet: 1.284 ± 0.028	1.758ValAsn: 1.758 ± 0.035	5.058ValPro: 5.058 ± 0.052	2.382ValGln: 2.382 ± 0.035	6.555ValArg: 6.555 ± 0.065	4.238ValSer: 4.238 ± 0.047	5.524ValThr: 5.524 ± 0.054	7.494ValVal: 7.494 ± 0.073	1.059ValTrp: 1.059 ± 0.025	1.547ValTyr: 1.547 ± 0.03	0.001ValXaa: 0.001 ± 0.001
Trp	1.823TrpAla: 1.823 ± 0.034	0.16TrpCys: 0.16 ± 0.009	0.825TrpAsp: 0.825 ± 0.022	0.74TrpGlu: 0.74 ± 0.022	0.501TrpPhe: 0.501 ± 0.015	1.058TrpGly: 1.058 ± 0.025	0.414TrpHis: 0.414 ± 0.015	0.542TrpIle: 0.542 ± 0.017	0.331TrpLys: 0.331 ± 0.013	1.893TrpLeu: 1.893 ± 0.038	0.251TrpMet: 0.251 ± 0.012	0.421TrpAsn: 0.421 ± 0.016	0.819TrpPro: 0.819 ± 0.022	0.761TrpGln: 0.761 ± 0.021	1.271TrpArg: 1.271 ± 0.024	1.012TrpSer: 1.012 ± 0.023	1.085TrpThr: 1.085 ± 0.024	1.063TrpVal: 1.063 ± 0.026	0.349TrpTrp: 0.349 ± 0.013	0.405TrpTyr: 0.405 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.649TyrAla: 2.649 ± 0.036	0.197TyrCys: 0.197 ± 0.009	1.353TyrAsp: 1.353 ± 0.035	1.067TyrGlu: 1.067 ± 0.022	0.679TyrPhe: 0.679 ± 0.019	2.314TyrGly: 2.314 ± 0.031	0.408TyrHis: 0.408 ± 0.016	0.432TyrIle: 0.432 ± 0.014	0.353TyrLys: 0.353 ± 0.015	2.454TyrLeu: 2.454 ± 0.036	0.22TyrMet: 0.22 ± 0.012	0.458TyrAsn: 0.458 ± 0.021	1.202TyrPro: 1.202 ± 0.025	0.837TyrGln: 0.837 ± 0.023	1.974TyrArg: 1.974 ± 0.034	1.039TyrSer: 1.039 ± 0.026	1.218TyrThr: 1.218 ± 0.034	1.562TyrVal: 1.562 ± 0.029	0.375TyrTrp: 0.375 ± 0.013	0.501TyrTyr: 0.501 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.001	0.0XaaHis: 0.0 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.004XaaPro: 0.004 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.0XaaSer: 0.0 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.001	0.022XaaXaa: 0.022 ± 0.005

Statistics based on 6308 proteins (2030878 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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