Streptomyces qinglanensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5156 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E7K3R7|A0A1E7K3R7_9ACTN RNA helicase OS=Streptomyces qinglanensis OX=943816 GN=AN217_12945 PE=4 SV=1
MM1 pKa = 7.67SYY3 pKa = 11.36ANPDD7 pKa = 4.0PDD9 pKa = 4.98PDD11 pKa = 3.83TTTGLEE17 pKa = 4.23PGGEE21 pKa = 4.27VPPGEE26 pKa = 4.26TPPAEE31 pKa = 4.44GSTSEE36 pKa = 4.0SGPFEE41 pKa = 3.99KK42 pKa = 10.62HH43 pKa = 5.41NPATGWSVVPVVLITLVVVAVLLFFVGYY71 pKa = 9.99LVEE74 pKa = 4.22LQQ76 pKa = 3.37

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E7KE74|A0A1E7KE74_9ACTN Uncharacterized protein OS=Streptomyces qinglanensis OX=943816 GN=AN217_02945 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.81GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5156

0

5156

1763939

45

21241

342.1

36.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.112 ± 0.066

0.805 ± 0.013

5.79 ± 0.027

6.101 ± 0.033

2.621 ± 0.016

9.975 ± 0.04

2.319 ± 0.015

2.667 ± 0.029

1.979 ± 0.034

10.425 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.671 ± 0.015

1.557 ± 0.018

6.409 ± 0.044

2.767 ± 0.023

8.528 ± 0.038

4.995 ± 0.028

5.752 ± 0.027

8.185 ± 0.034

1.42 ± 0.015

1.922 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski