Grapevine Algerian latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4VMI3|Q4VMI3_9TOMB 24K protein OS=Grapevine Algerian latent virus OX=208084 PE=3 SV=1
MM1 pKa = 7.56EE2 pKa = 4.39RR3 pKa = 11.84TIQGSDD9 pKa = 2.98VRR11 pKa = 11.84EE12 pKa = 3.96QANSEE17 pKa = 4.04RR18 pKa = 11.84WDD20 pKa = 3.89GGCGSTITPFKK31 pKa = 10.92LPDD34 pKa = 3.83EE35 pKa = 4.7SPSLYY40 pKa = 9.74EE41 pKa = 3.78WRR43 pKa = 11.84LHH45 pKa = 5.75NSEE48 pKa = 4.05EE49 pKa = 5.04SEE51 pKa = 5.1DD52 pKa = 3.73KK53 pKa = 11.04DD54 pKa = 3.5HH55 pKa = 7.42PLGFKK60 pKa = 10.31EE61 pKa = 4.35SWCFGKK67 pKa = 10.66VVFKK71 pKa = 10.78RR72 pKa = 11.84YY73 pKa = 9.63LRR75 pKa = 11.84YY76 pKa = 10.51DD77 pKa = 3.22GTEE80 pKa = 3.86ASLHH84 pKa = 5.89RR85 pKa = 11.84ALGSWEE91 pKa = 3.78RR92 pKa = 11.84SSVNDD97 pKa = 3.35AASRR101 pKa = 11.84FLGLGQIGCTYY112 pKa = 10.51SIRR115 pKa = 11.84FRR117 pKa = 11.84GSCLTLSGGSRR128 pKa = 11.84TLQRR132 pKa = 11.84LIEE135 pKa = 3.86MAIRR139 pKa = 11.84TKK141 pKa = 10.39CTMLQLAPCEE151 pKa = 4.27VEE153 pKa = 4.05SDD155 pKa = 3.57VSRR158 pKa = 11.84RR159 pKa = 11.84CPEE162 pKa = 3.55GTEE165 pKa = 3.98AFEE168 pKa = 4.92KK169 pKa = 10.64EE170 pKa = 4.38SEE172 pKa = 4.12

Molecular weight: 19.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4VMI6|Q4VMI6_9TOMB RNA-directed RNA polymerase OS=Grapevine Algerian latent virus OX=208084 PE=3 SV=1
MM1 pKa = 6.5EE2 pKa = 5.65TIRR5 pKa = 11.84RR6 pKa = 11.84MIWPKK11 pKa = 10.73KK12 pKa = 9.4EE13 pKa = 3.6IFVGDD18 pKa = 3.39FAIGVNRR25 pKa = 11.84TAPVDD30 pKa = 3.42IFQLVCRR37 pKa = 11.84VVLRR41 pKa = 11.84YY42 pKa = 8.72MRR44 pKa = 11.84TGKK47 pKa = 9.73IEE49 pKa = 4.3CDD51 pKa = 3.2SDD53 pKa = 4.34SMTKK57 pKa = 10.35FLVEE61 pKa = 4.67LLKK64 pKa = 10.15TDD66 pKa = 5.32CAAKK70 pKa = 10.03WEE72 pKa = 4.25WFMKK76 pKa = 9.78RR77 pKa = 11.84RR78 pKa = 11.84QRR80 pKa = 11.84GDD82 pKa = 3.49YY83 pKa = 10.1IVPLSIAALPVIPLLSYY100 pKa = 8.13ATKK103 pKa = 10.4VRR105 pKa = 11.84AVSVKK110 pKa = 10.68AFGNEE115 pKa = 3.29LSFNIRR121 pKa = 11.84VPRR124 pKa = 11.84PSVPKK129 pKa = 10.39KK130 pKa = 10.83GLLLRR135 pKa = 11.84LAAGLALAPICALAVYY151 pKa = 7.97ATLPRR156 pKa = 11.84EE157 pKa = 3.89KK158 pKa = 10.77LSVFKK163 pKa = 11.04LRR165 pKa = 11.84TEE167 pKa = 4.26ARR169 pKa = 11.84AHH171 pKa = 5.9MEE173 pKa = 4.18DD174 pKa = 3.5EE175 pKa = 5.34RR176 pKa = 11.84EE177 pKa = 4.2ATDD180 pKa = 3.71CLVVEE185 pKa = 5.21PARR188 pKa = 11.84EE189 pKa = 3.99LKK191 pKa = 10.99GKK193 pKa = 10.26DD194 pKa = 3.6GEE196 pKa = 4.5DD197 pKa = 3.55LLTGSRR203 pKa = 11.84MTKK206 pKa = 10.3VIASTGRR213 pKa = 11.84PRR215 pKa = 11.84RR216 pKa = 11.84RR217 pKa = 11.84PYY219 pKa = 9.67AAKK222 pKa = 9.43IAQVARR228 pKa = 11.84AKK230 pKa = 10.53VGYY233 pKa = 9.9LKK235 pKa = 10.75NSPEE239 pKa = 3.62NRR241 pKa = 11.84LIYY244 pKa = 9.49QRR246 pKa = 11.84VMIEE250 pKa = 5.34IMDD253 pKa = 4.32KK254 pKa = 11.03DD255 pKa = 3.68CVRR258 pKa = 11.84YY259 pKa = 9.97VDD261 pKa = 4.33RR262 pKa = 11.84DD263 pKa = 3.8VILPLAIGCCFVYY276 pKa = 10.38PDD278 pKa = 4.17GVEE281 pKa = 3.91EE282 pKa = 5.03SAALWGSQEE291 pKa = 4.12SLGVKK296 pKa = 9.67

Molecular weight: 33.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1872

172

818

374.4

41.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.28 ± 0.78

2.137 ± 0.465

4.861 ± 0.255

5.769 ± 1.061

3.419 ± 0.321

7.479 ± 0.774

1.496 ± 0.447

4.38 ± 0.358

5.823 ± 0.703

9.776 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.128

3.259 ± 0.717

4.541 ± 0.345

2.778 ± 0.267

7.853 ± 0.76

6.143 ± 0.895

6.036 ± 1.444

8.707 ± 0.696

1.442 ± 0.137

3.152 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski