Aspergillus violaceofuscus (strain CBS 115571)
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12074 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V5HIV6|A0A2V5HIV6_ASPV1 GST N-terminal domain-containing protein OS=Aspergillus violaceofuscus (strain CBS 115571) OX=1450538 GN=BO99DRAFT_400523 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 10.23 HH3 pKa = 6.08 IDD5 pKa = 3.69 KK6 pKa = 10.6 LALAAACLPGMVLKK20 pKa = 10.5 AAPLSEE26 pKa = 4.33 DD27 pKa = 3.4 TANAPSQAGEE37 pKa = 4.36 TEE39 pKa = 4.21 PVDD42 pKa = 3.55 QGAEE46 pKa = 4.13 STAEE50 pKa = 3.76 ASAAAAAATPGFCDD64 pKa = 4.66 CEE66 pKa = 4.47 SSNCTDD72 pKa = 3.01 VEE74 pKa = 4.24 AGGRR78 pKa = 11.84 GAGAGCDD85 pKa = 3.69 GCPGGDD91 pKa = 3.12 SVSPDD96 pKa = 2.62 GRR98 pKa = 11.84 GGYY101 pKa = 7.62 NTLGNGTANSTGNGSGDD118 pKa = 3.86 CADD121 pKa = 3.8 VEE123 pKa = 4.35 AGGRR127 pKa = 11.84 GDD129 pKa = 3.96 GNADD133 pKa = 3.59 GTDD136 pKa = 4.07 DD137 pKa = 4.71 GCDD140 pKa = 3.45 DD141 pKa = 4.23 ANANAGSSDD150 pKa = 2.86 GRR152 pKa = 11.84 GGFRR156 pKa = 11.84 LLCIGSPDD164 pKa = 4.12 ADD166 pKa = 4.57 PIATVDD172 pKa = 3.74 LFF174 pKa = 4.09
Molecular weight: 16.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.668
IPC_protein 3.681
Toseland 3.452
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.503
Grimsley 3.363
Solomon 3.681
Lehninger 3.63
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.808
Patrickios 0.985
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.729
Protein with the highest isoelectric point:
>tr|A0A2V5HJT7|A0A2V5HJT7_ASPV1 Uncharacterized protein OS=Aspergillus violaceofuscus (strain CBS 115571) OX=1450538 GN=BO99DRAFT_321125 PE=4 SV=1
SS1 pKa = 6.85 HH2 pKa = 7.5 KK3 pKa = 10.71 SFRR6 pKa = 11.84 TKK8 pKa = 10.45 QKK10 pKa = 9.54 LAKK13 pKa = 9.74 AQRR16 pKa = 11.84 QNRR19 pKa = 11.84 PIPQWIRR26 pKa = 11.84 LRR28 pKa = 11.84 TGNTIRR34 pKa = 11.84 YY35 pKa = 5.79 NAKK38 pKa = 8.89 RR39 pKa = 11.84 RR40 pKa = 11.84 HH41 pKa = 4.14 WRR43 pKa = 11.84 KK44 pKa = 7.41 TRR46 pKa = 11.84 LGII49 pKa = 4.46
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12074
0
12074
5671855
49
7770
469.8
51.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.011 ± 0.021
1.326 ± 0.01
5.504 ± 0.016
5.975 ± 0.018
3.691 ± 0.015
6.847 ± 0.02
2.553 ± 0.01
4.726 ± 0.017
4.092 ± 0.017
9.311 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.09 ± 0.008
3.366 ± 0.012
6.125 ± 0.026
4.11 ± 0.018
6.302 ± 0.02
8.143 ± 0.025
6.154 ± 0.016
6.311 ± 0.018
1.517 ± 0.008
2.843 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here