Lysobacter soli
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3530 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3D8V8D0|A0A3D8V8D0_9GAMM Thymidine kinase OS=Lysobacter soli OX=453783 GN=tdk PE=3 SV=1
MM1 pKa = 7.96 SEE3 pKa = 3.52 IAATTTFRR11 pKa = 11.84 TWMCVVCGFIYY22 pKa = 10.64 DD23 pKa = 3.83 EE24 pKa = 4.46 ALGLPEE30 pKa = 4.88 EE31 pKa = 5.36 GIAPGTRR38 pKa = 11.84 WEE40 pKa = 4.7 DD41 pKa = 3.78 VPDD44 pKa = 3.14 TWTCPDD50 pKa = 3.91 CGVTKK55 pKa = 10.65 DD56 pKa = 4.35 DD57 pKa = 4.54 FEE59 pKa = 4.47 MMPLL63 pKa = 3.56
Molecular weight: 7.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.777
IPC2_protein 3.77
IPC_protein 3.656
Toseland 3.465
ProMoST 3.846
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.389
Solomon 3.617
Lehninger 3.567
Nozaki 3.795
DTASelect 3.948
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.783
Patrickios 0.604
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A3D8VF42|A0A3D8VF42_9GAMM 2-dehydro-3-deoxy-6-phosphogalactonate aldolase OS=Lysobacter soli OX=453783 GN=DX912_09245 PE=3 SV=1
MM1 pKa = 7.35 SAGRR5 pKa = 11.84 RR6 pKa = 11.84 LASRR10 pKa = 11.84 ATAWQAAAAALAALAFIPLGAPMALAAAVGGGAVVAGGLVAALMALGGGIQPAGMAMGRR69 pKa = 11.84 LLAGVMLKK77 pKa = 9.58 WVVVFGVLTLGLAGFKK93 pKa = 10.49 LPPLPMLVGVLAATLAFVLANLLKK117 pKa = 10.59 RR118 pKa = 4.01
Molecular weight: 11.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.404
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.115
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.07
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3530
0
3530
1159365
30
2743
328.4
35.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.899 ± 0.059
0.789 ± 0.012
6.12 ± 0.04
5.437 ± 0.039
3.497 ± 0.025
8.454 ± 0.043
2.237 ± 0.022
4.179 ± 0.026
2.854 ± 0.036
10.334 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.218 ± 0.019
2.57 ± 0.029
5.341 ± 0.032
3.493 ± 0.025
7.827 ± 0.048
5.128 ± 0.028
5.153 ± 0.034
7.624 ± 0.029
1.546 ± 0.017
2.301 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here