Dioscorea nummularia-associated virus
Average proteome isoelectric point is 7.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346RP35|A0A346RP35_9VIRU ORF4 OS=Dioscorea nummularia-associated virus OX=2303485 PE=4 SV=1
MM1 pKa = 7.77 LFGKK5 pKa = 7.9 TLNCKK10 pKa = 10.13 LKK12 pKa = 10.29 PIKK15 pKa = 10.53 EE16 pKa = 4.08 NMDD19 pKa = 3.51 NKK21 pKa = 10.24 STVILPTKK29 pKa = 10.27 DD30 pKa = 2.79 IIRR33 pKa = 11.84 SYY35 pKa = 10.92 RR36 pKa = 11.84 NQIFPKK42 pKa = 9.88 KK43 pKa = 9.1 KK44 pKa = 8.03 NKK46 pKa = 9.99 EE47 pKa = 3.91 IIIEE51 pKa = 4.01 NLLNPEE57 pKa = 3.97 QQLDD61 pKa = 3.78 LARR64 pKa = 11.84 SKK66 pKa = 9.91 QAKK69 pKa = 9.58 LIPAEE74 pKa = 3.86 VLYY77 pKa = 10.47 QHH79 pKa = 6.71 GFWEE83 pKa = 4.76 TKK85 pKa = 8.89 PKK87 pKa = 10.52 VYY89 pKa = 10.26 QHH91 pKa = 6.09 YY92 pKa = 9.66 SEE94 pKa = 5.68 EE95 pKa = 4.53 IISCCDD101 pKa = 2.77 TTTQIDD107 pKa = 4.21 LKK109 pKa = 10.82 FLSKK113 pKa = 10.63 NSKK116 pKa = 10.43 HH117 pKa = 5.76 EE118 pKa = 4.21 LLSKK122 pKa = 10.33 DD123 pKa = 3.32 YY124 pKa = 10.94 SIIHH128 pKa = 6.74 IGLILIGIHH137 pKa = 5.4 GLHH140 pKa = 6.33 RR141 pKa = 11.84 RR142 pKa = 11.84 NYY144 pKa = 8.27 GSKK147 pKa = 10.95 VMIALADD154 pKa = 3.61 TSDD157 pKa = 4.77 NIPQKK162 pKa = 10.95 AIIGSMEE169 pKa = 3.53 VDD171 pKa = 2.69 MGEE174 pKa = 4.01 NHH176 pKa = 6.26 EE177 pKa = 4.52 LCYY180 pKa = 9.78 FAPDD184 pKa = 3.16 MSMTIQDD191 pKa = 3.7 FCNYY195 pKa = 9.18 FSLIIKK201 pKa = 7.79 TKK203 pKa = 9.52 GYY205 pKa = 8.43 EE206 pKa = 4.33 TMSHH210 pKa = 6.9 RR211 pKa = 11.84 DD212 pKa = 3.15 NLLITKK218 pKa = 10.05 ALVGRR223 pKa = 11.84 IGNNSSSAYY232 pKa = 9.24 RR233 pKa = 11.84 LKK235 pKa = 10.48 IEE237 pKa = 4.55 NVLTHH242 pKa = 7.27 LATQGVRR249 pKa = 11.84 AIEE252 pKa = 4.23 GKK254 pKa = 10.28 KK255 pKa = 10.54 FSSTDD260 pKa = 3.29 YY261 pKa = 11.59 DD262 pKa = 3.5 EE263 pKa = 5.26 TEE265 pKa = 3.83 WQISLKK271 pKa = 9.85 EE272 pKa = 4.11 KK273 pKa = 10.84 KK274 pKa = 10.31 KK275 pKa = 10.36 MPTKK279 pKa = 9.52 LQYY282 pKa = 9.32 WKK284 pKa = 9.57 EE285 pKa = 3.9 LSGEE289 pKa = 4.18 SSTATFTFTTPEE301 pKa = 3.78 EE302 pKa = 4.31 KK303 pKa = 10.06 PIKK306 pKa = 10.27 EE307 pKa = 4.26 EE308 pKa = 3.77 ISDD311 pKa = 4.0 EE312 pKa = 4.18 EE313 pKa = 4.45 TCLFFIDD320 pKa = 5.95 DD321 pKa = 4.84 YY322 pKa = 11.99 DD323 pKa = 5.16 DD324 pKa = 4.59 DD325 pKa = 4.06 WTSEE329 pKa = 3.97 EE330 pKa = 4.99 LEE332 pKa = 4.08 ILQEE336 pKa = 3.94 EE337 pKa = 4.53 LGVIIEE343 pKa = 4.29 EE344 pKa = 4.08 PLNIRR349 pKa = 11.84 RR350 pKa = 11.84 VHH352 pKa = 5.44 TPITEE357 pKa = 4.18 QVTEE361 pKa = 4.61 TISLQPLYY369 pKa = 10.75 EE370 pKa = 4.15 EE371 pKa = 4.65 EE372 pKa = 4.19 PLVNRR377 pKa = 11.84 DD378 pKa = 2.41 IYY380 pKa = 11.06 QRR382 pKa = 11.84 ILQEE386 pKa = 3.76 QNQEE390 pKa = 4.26 FEE392 pKa = 4.6 CPGRR396 pKa = 11.84 MQQLDD401 pKa = 3.88 QVLMEE406 pKa = 4.85 AEE408 pKa = 4.35 SEE410 pKa = 4.13 NRR412 pKa = 11.84 PTTSVPQTRR421 pKa = 11.84 VSGTPVFGPPALQPVQLPHH440 pKa = 7.1 TIIQPRR446 pKa = 11.84 GNVHH450 pKa = 5.33 TRR452 pKa = 11.84 PYY454 pKa = 10.23 RR455 pKa = 11.84 YY456 pKa = 10.09 DD457 pKa = 5.04 LIPTLPSANVQTGSMLVLPEE477 pKa = 3.56 ISKK480 pKa = 9.87 WEE482 pKa = 3.74 EE483 pKa = 3.6 TIRR486 pKa = 11.84 RR487 pKa = 11.84 WEE489 pKa = 4.44 SNTITHH495 pKa = 6.49 VATKK499 pKa = 10.5 QFTDD503 pKa = 3.34 NLDD506 pKa = 3.02 KK507 pKa = 11.42 VMYY510 pKa = 10.25 VEE512 pKa = 4.3 NLLGEE517 pKa = 4.34 TVKK520 pKa = 10.88 LYY522 pKa = 9.09 FQNWRR527 pKa = 11.84 TAFPTEE533 pKa = 4.12 FQALIDD539 pKa = 3.86 IAEE542 pKa = 4.44 DD543 pKa = 3.49 TANITSQIRR552 pKa = 11.84 QIILGYY558 pKa = 10.29 DD559 pKa = 3.66 EE560 pKa = 5.59 YY561 pKa = 11.17 RR562 pKa = 11.84 GQTEE566 pKa = 4.1 MQNQALLDD574 pKa = 3.98 LEE576 pKa = 4.24 QLQITNMKK584 pKa = 10.28 DD585 pKa = 2.56 IEE587 pKa = 4.54 AYY589 pKa = 8.6 STTFLALASRR599 pKa = 11.84 TGAAFLSPEE608 pKa = 3.35 ISAKK612 pKa = 9.09 YY613 pKa = 9.36 FRR615 pKa = 11.84 KK616 pKa = 10.14 LPPPFNIRR624 pKa = 11.84 IQEE627 pKa = 3.8 MWTEE631 pKa = 4.53 KK632 pKa = 10.43 FPNWLVGVAPRR643 pKa = 11.84 IDD645 pKa = 3.64 FTYY648 pKa = 10.65 KK649 pKa = 10.3 ILQDD653 pKa = 3.53 LCRR656 pKa = 11.84 NNEE659 pKa = 3.93 LHH661 pKa = 6.68 RR662 pKa = 11.84 QAKK665 pKa = 10.02 DD666 pKa = 3.51 FSFCRR671 pKa = 11.84 SIAVPGQYY679 pKa = 10.62 SRR681 pKa = 11.84 TTQPRR686 pKa = 11.84 KK687 pKa = 6.78 TLRR690 pKa = 11.84 RR691 pKa = 11.84 STRR694 pKa = 11.84 YY695 pKa = 9.24 KK696 pKa = 9.6 GHH698 pKa = 5.81 NVKK701 pKa = 10.56 SNQVRR706 pKa = 11.84 KK707 pKa = 9.67 FRR709 pKa = 11.84 TDD711 pKa = 2.73 KK712 pKa = 11.03 ATGKK716 pKa = 9.57 PSKK719 pKa = 9.89 CRR721 pKa = 11.84 CFICGEE727 pKa = 3.94 IGHH730 pKa = 6.94 FANQCQNRR738 pKa = 11.84 NVNRR742 pKa = 11.84 QRR744 pKa = 11.84 LAIYY748 pKa = 10.07 EE749 pKa = 4.17 EE750 pKa = 5.07 LDD752 pKa = 5.65 LEE754 pKa = 4.4 PQWDD758 pKa = 3.72 IVSLNDD764 pKa = 4.54 GEE766 pKa = 5.09 DD767 pKa = 3.76 PNDD770 pKa = 3.56 SDD772 pKa = 4.16 ICSFSDD778 pKa = 4.17 NEE780 pKa = 3.93 LDD782 pKa = 3.44 QQEE785 pKa = 4.3 RR786 pKa = 11.84 LEE788 pKa = 4.45 FPTEE792 pKa = 3.74 KK793 pKa = 10.38 FLMITLDD800 pKa = 3.93 SIDD803 pKa = 3.79 WNLQRR808 pKa = 11.84 ANKK811 pKa = 10.09 KK812 pKa = 8.57 LTTDD816 pKa = 3.38 QQKK819 pKa = 10.71 CMHH822 pKa = 6.6 NWNHH826 pKa = 6.23 HH827 pKa = 5.81 PDD829 pKa = 3.33 IDD831 pKa = 4.04 YY832 pKa = 11.36 LNNNCYY838 pKa = 9.88 FCRR841 pKa = 11.84 LRR843 pKa = 11.84 PPKK846 pKa = 9.92 RR847 pKa = 11.84 ARR849 pKa = 11.84 TICLNCNLLLCRR861 pKa = 11.84 YY862 pKa = 9.34 CAEE865 pKa = 4.39 LKK867 pKa = 10.89 LKK869 pKa = 10.55 IEE871 pKa = 4.46 VPLGQPPIPVSRR883 pKa = 11.84 VDD885 pKa = 3.17 HH886 pKa = 6.45 RR887 pKa = 11.84 RR888 pKa = 11.84 LILEE892 pKa = 4.04 QAQHH896 pKa = 5.02 ITEE899 pKa = 4.87 LEE901 pKa = 3.96 QEE903 pKa = 4.38 LAQQKK908 pKa = 9.73 QKK910 pKa = 11.24 EE911 pKa = 4.27 KK912 pKa = 10.98 DD913 pKa = 3.35 LEE915 pKa = 4.27 AQLLAEE921 pKa = 4.69 KK922 pKa = 10.8 LSGFQLGEE930 pKa = 3.98 SSEE933 pKa = 4.57 TIHH936 pKa = 6.16 MASIEE941 pKa = 4.1 DD942 pKa = 3.88 EE943 pKa = 4.33 EE944 pKa = 4.98 GVNALAAVQNNLLNFIAILEE964 pKa = 4.43 IGDD967 pKa = 3.63 KK968 pKa = 10.32 TFSLKK973 pKa = 10.75 AILDD977 pKa = 3.89 TGASGCCVQYY987 pKa = 10.98 SALPNYY993 pKa = 10.19 CYY995 pKa = 7.87 EE996 pKa = 4.26 TLPQPVNLHH1005 pKa = 5.58 GLNNIEE1011 pKa = 4.16 KK1012 pKa = 9.17 AKK1014 pKa = 9.85 MRR1016 pKa = 11.84 IRR1018 pKa = 11.84 SGNIILNKK1026 pKa = 10.12 DD1027 pKa = 3.7 KK1028 pKa = 11.39 YY1029 pKa = 9.54 PLPLTYY1035 pKa = 9.05 VTPVISGEE1043 pKa = 4.03 LQLVIGMNFIHH1054 pKa = 6.45 SFKK1057 pKa = 10.84 GGVKK1061 pKa = 9.59 IEE1063 pKa = 4.2 GTSVTFYY1070 pKa = 10.47 RR1071 pKa = 11.84 KK1072 pKa = 9.63 SDD1074 pKa = 3.34 ILQTSPIINKK1084 pKa = 9.32 NLVIGEE1090 pKa = 4.44 DD1091 pKa = 4.01 GEE1093 pKa = 5.06 DD1094 pKa = 3.45 VNQEE1098 pKa = 3.78 SMYY1101 pKa = 10.36 MIDD1104 pKa = 6.31 DD1105 pKa = 3.49 ILLYY1109 pKa = 10.68 NVGRR1113 pKa = 11.84 LTDD1116 pKa = 3.82 YY1117 pKa = 10.44 EE1118 pKa = 4.11 NQIIPILHH1126 pKa = 6.65 KK1127 pKa = 10.84 LEE1129 pKa = 4.74 EE1130 pKa = 4.04 IQIIGNDD1137 pKa = 3.6 PLKK1140 pKa = 10.65 YY1141 pKa = 9.38 WEE1143 pKa = 4.68 KK1144 pKa = 10.62 NQIRR1148 pKa = 11.84 CKK1150 pKa = 10.55 LDD1152 pKa = 3.57 IINPDD1157 pKa = 3.45 LTIQDD1162 pKa = 3.97 KK1163 pKa = 10.72 PIIPSPEE1170 pKa = 3.64 MAKK1173 pKa = 10.2 EE1174 pKa = 4.0 YY1175 pKa = 9.71 EE1176 pKa = 4.03 KK1177 pKa = 10.95 HH1178 pKa = 4.8 ITEE1181 pKa = 4.46 LLALKK1186 pKa = 9.78 VIRR1189 pKa = 11.84 PSQSRR1194 pKa = 11.84 HH1195 pKa = 3.44 RR1196 pKa = 11.84 TAAFIVNKK1204 pKa = 10.05 HH1205 pKa = 4.95 SEE1207 pKa = 4.16 QVRR1210 pKa = 11.84 GKK1212 pKa = 10.66 SRR1214 pKa = 11.84 MVYY1217 pKa = 9.74 NYY1219 pKa = 10.3 KK1220 pKa = 10.38 RR1221 pKa = 11.84 LNDD1224 pKa = 3.56 NTYY1227 pKa = 10.34 KK1228 pKa = 10.7 DD1229 pKa = 4.03 QYY1231 pKa = 8.96 TLPSIDD1237 pKa = 3.91 YY1238 pKa = 10.26 LLLKK1242 pKa = 10.43 IKK1244 pKa = 10.7 DD1245 pKa = 3.69 KK1246 pKa = 10.83 IVYY1249 pKa = 10.27 SKK1251 pKa = 11.1 FDD1253 pKa = 3.94 LKK1255 pKa = 11.25 SGFHH1259 pKa = 6.43 QIMMDD1264 pKa = 3.5 PQSIEE1269 pKa = 3.4 WTAFVCPQGHH1279 pKa = 5.92 FEE1281 pKa = 4.43 WIVMPFGLKK1290 pKa = 9.5 NAPSVFQRR1298 pKa = 11.84 KK1299 pKa = 7.38 MDD1301 pKa = 4.51 NIFKK1305 pKa = 10.33 KK1306 pKa = 10.4 YY1307 pKa = 10.48 SEE1309 pKa = 4.52 FVCVYY1314 pKa = 9.92 IDD1316 pKa = 4.98 DD1317 pKa = 3.91 ILIFSEE1323 pKa = 5.26 SIQQHH1328 pKa = 4.84 VQHH1331 pKa = 7.13 LLQFFQVCKK1340 pKa = 10.73 EE1341 pKa = 3.83 EE1342 pKa = 4.35 GLILSKK1348 pKa = 9.94 TKK1350 pKa = 10.91 LKK1352 pKa = 10.6 IGVANIEE1359 pKa = 4.07 FLGLEE1364 pKa = 3.73 IGEE1367 pKa = 4.82 GKK1369 pKa = 9.98 VQLQPHH1375 pKa = 6.0 ILKK1378 pKa = 10.68 NILEE1382 pKa = 4.31 FPEE1385 pKa = 4.65 DD1386 pKa = 3.38 QLEE1389 pKa = 4.32 TLKK1392 pKa = 11.11 GLQKK1396 pKa = 10.74 FLGILNYY1403 pKa = 9.97 ARR1405 pKa = 11.84 NYY1407 pKa = 9.56 IPNLSKK1413 pKa = 10.0 YY1414 pKa = 8.14 TRR1416 pKa = 11.84 IFYY1419 pKa = 9.66 NKK1421 pKa = 9.16 CSSKK1425 pKa = 11.05 GEE1427 pKa = 4.15 RR1428 pKa = 11.84 KK1429 pKa = 9.72 FNSQDD1434 pKa = 2.35 WKK1436 pKa = 9.26 MVRR1439 pKa = 11.84 KK1440 pKa = 9.23 IKK1442 pKa = 10.43 EE1443 pKa = 4.51 VITKK1447 pKa = 9.22 LPPLILPKK1455 pKa = 9.0 PQSYY1459 pKa = 10.31 IIIEE1463 pKa = 4.19 TDD1465 pKa = 3.12 GSLEE1469 pKa = 3.9 GWGGILKK1476 pKa = 9.01 WRR1478 pKa = 11.84 PSQQDD1483 pKa = 3.26 SPSTEE1488 pKa = 3.93 KK1489 pKa = 10.31 ISRR1492 pKa = 11.84 YY1493 pKa = 9.71 CSGSYY1498 pKa = 10.2 KK1499 pKa = 10.45 SAISAIDD1506 pKa = 3.8 AEE1508 pKa = 4.71 IMACIYY1514 pKa = 10.95 VLDD1517 pKa = 3.78 KK1518 pKa = 11.07 FKK1520 pKa = 11.02 IFLYY1524 pKa = 9.84 EE1525 pKa = 3.93 KK1526 pKa = 10.88 KK1527 pKa = 10.32 EE1528 pKa = 3.82 FTLRR1532 pKa = 11.84 TDD1534 pKa = 4.67 CIAIVNFYY1542 pKa = 11.42 NKK1544 pKa = 10.35 LNNKK1548 pKa = 8.81 KK1549 pKa = 10.59 LNINRR1554 pKa = 11.84 WVNFCDD1560 pKa = 5.23 LITGLGLTVKK1570 pKa = 10.08 IEE1572 pKa = 4.38 HH1573 pKa = 6.52 IQGKK1577 pKa = 10.11 DD1578 pKa = 3.04 NVGADD1583 pKa = 3.25 RR1584 pKa = 11.84 LSRR1587 pKa = 11.84 IIAASPYY1594 pKa = 9.92 RR1595 pKa = 3.86
Molecular weight: 184.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.178
IPC2_protein 6.262
IPC_protein 6.3
Toseland 6.465
ProMoST 6.722
Dawson 6.664
Bjellqvist 6.678
Wikipedia 6.664
Rodwell 6.649
Grimsley 6.561
Solomon 6.664
Lehninger 6.664
Nozaki 6.956
DTASelect 7.073
Thurlkill 7.102
EMBOSS 7.073
Sillero 7.059
Patrickios 4.228
IPC_peptide 6.678
IPC2_peptide 6.781
IPC2.peptide.svr19 6.783
Protein with the highest isoelectric point:
>tr|A0A346RP34|A0A346RP34_9VIRU ORF3 OS=Dioscorea nummularia-associated virus OX=2303485 PE=4 SV=1
MM1 pKa = 6.92 STEE4 pKa = 4.02 DD5 pKa = 4.8 LEE7 pKa = 5.63 LKK9 pKa = 9.56 LTLEE13 pKa = 4.51 KK14 pKa = 10.68 LSKK17 pKa = 10.07 TEE19 pKa = 3.91 FTPTQGFTKK28 pKa = 10.19 EE29 pKa = 4.12 VSQSNLIRR37 pKa = 11.84 QHH39 pKa = 4.53 NTIIEE44 pKa = 4.22 LLLTLHH50 pKa = 6.09 QKK52 pKa = 9.49 VDD54 pKa = 3.41 KK55 pKa = 9.84 LTQNVKK61 pKa = 10.23 QIQIKK66 pKa = 8.65 EE67 pKa = 4.25 AEE69 pKa = 4.17 LSSVVKK75 pKa = 10.62 NLQTKK80 pKa = 10.39 NSTEE84 pKa = 3.95 EE85 pKa = 4.02 KK86 pKa = 9.74 PVVKK90 pKa = 10.04 TSPKK94 pKa = 10.0 VFAVWEE100 pKa = 4.27 DD101 pKa = 3.36 PKK103 pKa = 11.3 LQVEE107 pKa = 4.71 AYY109 pKa = 8.31 KK110 pKa = 10.42 RR111 pKa = 11.84 KK112 pKa = 10.06 YY113 pKa = 8.02 GQQVYY118 pKa = 9.31 SHH120 pKa = 7.03 TSHH123 pKa = 7.19 KK124 pKa = 10.37 GHH126 pKa = 6.62 NN127 pKa = 3.64
Molecular weight: 14.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.589
IPC2_protein 8.565
IPC_protein 8.507
Toseland 9.516
ProMoST 9.063
Dawson 9.677
Bjellqvist 9.253
Wikipedia 9.78
Rodwell 10.292
Grimsley 9.706
Solomon 9.736
Lehninger 9.721
Nozaki 9.458
DTASelect 9.253
Thurlkill 9.531
EMBOSS 9.897
Sillero 9.575
Patrickios 9.926
IPC_peptide 9.736
IPC2_peptide 7.644
IPC2.peptide.svr19 7.577
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2368
127
1595
592.0
68.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.223 ± 0.755
1.858 ± 0.495
4.772 ± 0.644
7.855 ± 0.555
3.927 ± 0.312
4.307 ± 0.538
2.28 ± 0.529
8.488 ± 1.231
8.319 ± 1.283
10.346 ± 0.691
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.816 ± 0.251
5.659 ± 0.177
4.265 ± 0.838
5.954 ± 0.497
4.519 ± 0.531
6.25 ± 0.554
6.081 ± 0.8
4.223 ± 0.773
1.225 ± 0.253
3.632 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here