Dioscorea nummularia-associated virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Dioscovirus; Dioscorea nummularia associated virus

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A346RP35|A0A346RP35_9VIRU ORF4 OS=Dioscorea nummularia-associated virus OX=2303485 PE=4 SV=1
MM1 pKa = 7.77LFGKK5 pKa = 7.9TLNCKK10 pKa = 10.13LKK12 pKa = 10.29PIKK15 pKa = 10.53EE16 pKa = 4.08NMDD19 pKa = 3.51NKK21 pKa = 10.24STVILPTKK29 pKa = 10.27DD30 pKa = 2.79IIRR33 pKa = 11.84SYY35 pKa = 10.92RR36 pKa = 11.84NQIFPKK42 pKa = 9.88KK43 pKa = 9.1KK44 pKa = 8.03NKK46 pKa = 9.99EE47 pKa = 3.91IIIEE51 pKa = 4.01NLLNPEE57 pKa = 3.97QQLDD61 pKa = 3.78LARR64 pKa = 11.84SKK66 pKa = 9.91QAKK69 pKa = 9.58LIPAEE74 pKa = 3.86VLYY77 pKa = 10.47QHH79 pKa = 6.71GFWEE83 pKa = 4.76TKK85 pKa = 8.89PKK87 pKa = 10.52VYY89 pKa = 10.26QHH91 pKa = 6.09YY92 pKa = 9.66SEE94 pKa = 5.68EE95 pKa = 4.53IISCCDD101 pKa = 2.77TTTQIDD107 pKa = 4.21LKK109 pKa = 10.82FLSKK113 pKa = 10.63NSKK116 pKa = 10.43HH117 pKa = 5.76EE118 pKa = 4.21LLSKK122 pKa = 10.33DD123 pKa = 3.32YY124 pKa = 10.94SIIHH128 pKa = 6.74IGLILIGIHH137 pKa = 5.4GLHH140 pKa = 6.33RR141 pKa = 11.84RR142 pKa = 11.84NYY144 pKa = 8.27GSKK147 pKa = 10.95VMIALADD154 pKa = 3.61TSDD157 pKa = 4.77NIPQKK162 pKa = 10.95AIIGSMEE169 pKa = 3.53VDD171 pKa = 2.69MGEE174 pKa = 4.01NHH176 pKa = 6.26EE177 pKa = 4.52LCYY180 pKa = 9.78FAPDD184 pKa = 3.16MSMTIQDD191 pKa = 3.7FCNYY195 pKa = 9.18FSLIIKK201 pKa = 7.79TKK203 pKa = 9.52GYY205 pKa = 8.43EE206 pKa = 4.33TMSHH210 pKa = 6.9RR211 pKa = 11.84DD212 pKa = 3.15NLLITKK218 pKa = 10.05ALVGRR223 pKa = 11.84IGNNSSSAYY232 pKa = 9.24RR233 pKa = 11.84LKK235 pKa = 10.48IEE237 pKa = 4.55NVLTHH242 pKa = 7.27LATQGVRR249 pKa = 11.84AIEE252 pKa = 4.23GKK254 pKa = 10.28KK255 pKa = 10.54FSSTDD260 pKa = 3.29YY261 pKa = 11.59DD262 pKa = 3.5EE263 pKa = 5.26TEE265 pKa = 3.83WQISLKK271 pKa = 9.85EE272 pKa = 4.11KK273 pKa = 10.84KK274 pKa = 10.31KK275 pKa = 10.36MPTKK279 pKa = 9.52LQYY282 pKa = 9.32WKK284 pKa = 9.57EE285 pKa = 3.9LSGEE289 pKa = 4.18SSTATFTFTTPEE301 pKa = 3.78EE302 pKa = 4.31KK303 pKa = 10.06PIKK306 pKa = 10.27EE307 pKa = 4.26EE308 pKa = 3.77ISDD311 pKa = 4.0EE312 pKa = 4.18EE313 pKa = 4.45TCLFFIDD320 pKa = 5.95DD321 pKa = 4.84YY322 pKa = 11.99DD323 pKa = 5.16DD324 pKa = 4.59DD325 pKa = 4.06WTSEE329 pKa = 3.97EE330 pKa = 4.99LEE332 pKa = 4.08ILQEE336 pKa = 3.94EE337 pKa = 4.53LGVIIEE343 pKa = 4.29EE344 pKa = 4.08PLNIRR349 pKa = 11.84RR350 pKa = 11.84VHH352 pKa = 5.44TPITEE357 pKa = 4.18QVTEE361 pKa = 4.61TISLQPLYY369 pKa = 10.75EE370 pKa = 4.15EE371 pKa = 4.65EE372 pKa = 4.19PLVNRR377 pKa = 11.84DD378 pKa = 2.41IYY380 pKa = 11.06QRR382 pKa = 11.84ILQEE386 pKa = 3.76QNQEE390 pKa = 4.26FEE392 pKa = 4.6CPGRR396 pKa = 11.84MQQLDD401 pKa = 3.88QVLMEE406 pKa = 4.85AEE408 pKa = 4.35SEE410 pKa = 4.13NRR412 pKa = 11.84PTTSVPQTRR421 pKa = 11.84VSGTPVFGPPALQPVQLPHH440 pKa = 7.1TIIQPRR446 pKa = 11.84GNVHH450 pKa = 5.33TRR452 pKa = 11.84PYY454 pKa = 10.23RR455 pKa = 11.84YY456 pKa = 10.09DD457 pKa = 5.04LIPTLPSANVQTGSMLVLPEE477 pKa = 3.56ISKK480 pKa = 9.87WEE482 pKa = 3.74EE483 pKa = 3.6TIRR486 pKa = 11.84RR487 pKa = 11.84WEE489 pKa = 4.44SNTITHH495 pKa = 6.49VATKK499 pKa = 10.5QFTDD503 pKa = 3.34NLDD506 pKa = 3.02KK507 pKa = 11.42VMYY510 pKa = 10.25VEE512 pKa = 4.3NLLGEE517 pKa = 4.34TVKK520 pKa = 10.88LYY522 pKa = 9.09FQNWRR527 pKa = 11.84TAFPTEE533 pKa = 4.12FQALIDD539 pKa = 3.86IAEE542 pKa = 4.44DD543 pKa = 3.49TANITSQIRR552 pKa = 11.84QIILGYY558 pKa = 10.29DD559 pKa = 3.66EE560 pKa = 5.59YY561 pKa = 11.17RR562 pKa = 11.84GQTEE566 pKa = 4.1MQNQALLDD574 pKa = 3.98LEE576 pKa = 4.24QLQITNMKK584 pKa = 10.28DD585 pKa = 2.56IEE587 pKa = 4.54AYY589 pKa = 8.6STTFLALASRR599 pKa = 11.84TGAAFLSPEE608 pKa = 3.35ISAKK612 pKa = 9.09YY613 pKa = 9.36FRR615 pKa = 11.84KK616 pKa = 10.14LPPPFNIRR624 pKa = 11.84IQEE627 pKa = 3.8MWTEE631 pKa = 4.53KK632 pKa = 10.43FPNWLVGVAPRR643 pKa = 11.84IDD645 pKa = 3.64FTYY648 pKa = 10.65KK649 pKa = 10.3ILQDD653 pKa = 3.53LCRR656 pKa = 11.84NNEE659 pKa = 3.93LHH661 pKa = 6.68RR662 pKa = 11.84QAKK665 pKa = 10.02DD666 pKa = 3.51FSFCRR671 pKa = 11.84SIAVPGQYY679 pKa = 10.62SRR681 pKa = 11.84TTQPRR686 pKa = 11.84KK687 pKa = 6.78TLRR690 pKa = 11.84RR691 pKa = 11.84STRR694 pKa = 11.84YY695 pKa = 9.24KK696 pKa = 9.6GHH698 pKa = 5.81NVKK701 pKa = 10.56SNQVRR706 pKa = 11.84KK707 pKa = 9.67FRR709 pKa = 11.84TDD711 pKa = 2.73KK712 pKa = 11.03ATGKK716 pKa = 9.57PSKK719 pKa = 9.89CRR721 pKa = 11.84CFICGEE727 pKa = 3.94IGHH730 pKa = 6.94FANQCQNRR738 pKa = 11.84NVNRR742 pKa = 11.84QRR744 pKa = 11.84LAIYY748 pKa = 10.07EE749 pKa = 4.17EE750 pKa = 5.07LDD752 pKa = 5.65LEE754 pKa = 4.4PQWDD758 pKa = 3.72IVSLNDD764 pKa = 4.54GEE766 pKa = 5.09DD767 pKa = 3.76PNDD770 pKa = 3.56SDD772 pKa = 4.16ICSFSDD778 pKa = 4.17NEE780 pKa = 3.93LDD782 pKa = 3.44QQEE785 pKa = 4.3RR786 pKa = 11.84LEE788 pKa = 4.45FPTEE792 pKa = 3.74KK793 pKa = 10.38FLMITLDD800 pKa = 3.93SIDD803 pKa = 3.79WNLQRR808 pKa = 11.84ANKK811 pKa = 10.09KK812 pKa = 8.57LTTDD816 pKa = 3.38QQKK819 pKa = 10.71CMHH822 pKa = 6.6NWNHH826 pKa = 6.23HH827 pKa = 5.81PDD829 pKa = 3.33IDD831 pKa = 4.04YY832 pKa = 11.36LNNNCYY838 pKa = 9.88FCRR841 pKa = 11.84LRR843 pKa = 11.84PPKK846 pKa = 9.92RR847 pKa = 11.84ARR849 pKa = 11.84TICLNCNLLLCRR861 pKa = 11.84YY862 pKa = 9.34CAEE865 pKa = 4.39LKK867 pKa = 10.89LKK869 pKa = 10.55IEE871 pKa = 4.46VPLGQPPIPVSRR883 pKa = 11.84VDD885 pKa = 3.17HH886 pKa = 6.45RR887 pKa = 11.84RR888 pKa = 11.84LILEE892 pKa = 4.04QAQHH896 pKa = 5.02ITEE899 pKa = 4.87LEE901 pKa = 3.96QEE903 pKa = 4.38LAQQKK908 pKa = 9.73QKK910 pKa = 11.24EE911 pKa = 4.27KK912 pKa = 10.98DD913 pKa = 3.35LEE915 pKa = 4.27AQLLAEE921 pKa = 4.69KK922 pKa = 10.8LSGFQLGEE930 pKa = 3.98SSEE933 pKa = 4.57TIHH936 pKa = 6.16MASIEE941 pKa = 4.1DD942 pKa = 3.88EE943 pKa = 4.33EE944 pKa = 4.98GVNALAAVQNNLLNFIAILEE964 pKa = 4.43IGDD967 pKa = 3.63KK968 pKa = 10.32TFSLKK973 pKa = 10.75AILDD977 pKa = 3.89TGASGCCVQYY987 pKa = 10.98SALPNYY993 pKa = 10.19CYY995 pKa = 7.87EE996 pKa = 4.26TLPQPVNLHH1005 pKa = 5.58GLNNIEE1011 pKa = 4.16KK1012 pKa = 9.17AKK1014 pKa = 9.85MRR1016 pKa = 11.84IRR1018 pKa = 11.84SGNIILNKK1026 pKa = 10.12DD1027 pKa = 3.7KK1028 pKa = 11.39YY1029 pKa = 9.54PLPLTYY1035 pKa = 9.05VTPVISGEE1043 pKa = 4.03LQLVIGMNFIHH1054 pKa = 6.45SFKK1057 pKa = 10.84GGVKK1061 pKa = 9.59IEE1063 pKa = 4.2GTSVTFYY1070 pKa = 10.47RR1071 pKa = 11.84KK1072 pKa = 9.63SDD1074 pKa = 3.34ILQTSPIINKK1084 pKa = 9.32NLVIGEE1090 pKa = 4.44DD1091 pKa = 4.01GEE1093 pKa = 5.06DD1094 pKa = 3.45VNQEE1098 pKa = 3.78SMYY1101 pKa = 10.36MIDD1104 pKa = 6.31DD1105 pKa = 3.49ILLYY1109 pKa = 10.68NVGRR1113 pKa = 11.84LTDD1116 pKa = 3.82YY1117 pKa = 10.44EE1118 pKa = 4.11NQIIPILHH1126 pKa = 6.65KK1127 pKa = 10.84LEE1129 pKa = 4.74EE1130 pKa = 4.04IQIIGNDD1137 pKa = 3.6PLKK1140 pKa = 10.65YY1141 pKa = 9.38WEE1143 pKa = 4.68KK1144 pKa = 10.62NQIRR1148 pKa = 11.84CKK1150 pKa = 10.55LDD1152 pKa = 3.57IINPDD1157 pKa = 3.45LTIQDD1162 pKa = 3.97KK1163 pKa = 10.72PIIPSPEE1170 pKa = 3.64MAKK1173 pKa = 10.2EE1174 pKa = 4.0YY1175 pKa = 9.71EE1176 pKa = 4.03KK1177 pKa = 10.95HH1178 pKa = 4.8ITEE1181 pKa = 4.46LLALKK1186 pKa = 9.78VIRR1189 pKa = 11.84PSQSRR1194 pKa = 11.84HH1195 pKa = 3.44RR1196 pKa = 11.84TAAFIVNKK1204 pKa = 10.05HH1205 pKa = 4.95SEE1207 pKa = 4.16QVRR1210 pKa = 11.84GKK1212 pKa = 10.66SRR1214 pKa = 11.84MVYY1217 pKa = 9.74NYY1219 pKa = 10.3KK1220 pKa = 10.38RR1221 pKa = 11.84LNDD1224 pKa = 3.56NTYY1227 pKa = 10.34KK1228 pKa = 10.7DD1229 pKa = 4.03QYY1231 pKa = 8.96TLPSIDD1237 pKa = 3.91YY1238 pKa = 10.26LLLKK1242 pKa = 10.43IKK1244 pKa = 10.7DD1245 pKa = 3.69KK1246 pKa = 10.83IVYY1249 pKa = 10.27SKK1251 pKa = 11.1FDD1253 pKa = 3.94LKK1255 pKa = 11.25SGFHH1259 pKa = 6.43QIMMDD1264 pKa = 3.5PQSIEE1269 pKa = 3.4WTAFVCPQGHH1279 pKa = 5.92FEE1281 pKa = 4.43WIVMPFGLKK1290 pKa = 9.5NAPSVFQRR1298 pKa = 11.84KK1299 pKa = 7.38MDD1301 pKa = 4.51NIFKK1305 pKa = 10.33KK1306 pKa = 10.4YY1307 pKa = 10.48SEE1309 pKa = 4.52FVCVYY1314 pKa = 9.92IDD1316 pKa = 4.98DD1317 pKa = 3.91ILIFSEE1323 pKa = 5.26SIQQHH1328 pKa = 4.84VQHH1331 pKa = 7.13LLQFFQVCKK1340 pKa = 10.73EE1341 pKa = 3.83EE1342 pKa = 4.35GLILSKK1348 pKa = 9.94TKK1350 pKa = 10.91LKK1352 pKa = 10.6IGVANIEE1359 pKa = 4.07FLGLEE1364 pKa = 3.73IGEE1367 pKa = 4.82GKK1369 pKa = 9.98VQLQPHH1375 pKa = 6.0ILKK1378 pKa = 10.68NILEE1382 pKa = 4.31FPEE1385 pKa = 4.65DD1386 pKa = 3.38QLEE1389 pKa = 4.32TLKK1392 pKa = 11.11GLQKK1396 pKa = 10.74FLGILNYY1403 pKa = 9.97ARR1405 pKa = 11.84NYY1407 pKa = 9.56IPNLSKK1413 pKa = 10.0YY1414 pKa = 8.14TRR1416 pKa = 11.84IFYY1419 pKa = 9.66NKK1421 pKa = 9.16CSSKK1425 pKa = 11.05GEE1427 pKa = 4.15RR1428 pKa = 11.84KK1429 pKa = 9.72FNSQDD1434 pKa = 2.35WKK1436 pKa = 9.26MVRR1439 pKa = 11.84KK1440 pKa = 9.23IKK1442 pKa = 10.43EE1443 pKa = 4.51VITKK1447 pKa = 9.22LPPLILPKK1455 pKa = 9.0PQSYY1459 pKa = 10.31IIIEE1463 pKa = 4.19TDD1465 pKa = 3.12GSLEE1469 pKa = 3.9GWGGILKK1476 pKa = 9.01WRR1478 pKa = 11.84PSQQDD1483 pKa = 3.26SPSTEE1488 pKa = 3.93KK1489 pKa = 10.31ISRR1492 pKa = 11.84YY1493 pKa = 9.71CSGSYY1498 pKa = 10.2KK1499 pKa = 10.45SAISAIDD1506 pKa = 3.8AEE1508 pKa = 4.71IMACIYY1514 pKa = 10.95VLDD1517 pKa = 3.78KK1518 pKa = 11.07FKK1520 pKa = 11.02IFLYY1524 pKa = 9.84EE1525 pKa = 3.93KK1526 pKa = 10.88KK1527 pKa = 10.32EE1528 pKa = 3.82FTLRR1532 pKa = 11.84TDD1534 pKa = 4.67CIAIVNFYY1542 pKa = 11.42NKK1544 pKa = 10.35LNNKK1548 pKa = 8.81KK1549 pKa = 10.59LNINRR1554 pKa = 11.84WVNFCDD1560 pKa = 5.23LITGLGLTVKK1570 pKa = 10.08IEE1572 pKa = 4.38HH1573 pKa = 6.52IQGKK1577 pKa = 10.11DD1578 pKa = 3.04NVGADD1583 pKa = 3.25RR1584 pKa = 11.84LSRR1587 pKa = 11.84IIAASPYY1594 pKa = 9.92RR1595 pKa = 3.86

Molecular weight:
184.21 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A346RP34|A0A346RP34_9VIRU ORF3 OS=Dioscorea nummularia-associated virus OX=2303485 PE=4 SV=1
MM1 pKa = 6.92STEE4 pKa = 4.02DD5 pKa = 4.8LEE7 pKa = 5.63LKK9 pKa = 9.56LTLEE13 pKa = 4.51KK14 pKa = 10.68LSKK17 pKa = 10.07TEE19 pKa = 3.91FTPTQGFTKK28 pKa = 10.19EE29 pKa = 4.12VSQSNLIRR37 pKa = 11.84QHH39 pKa = 4.53NTIIEE44 pKa = 4.22LLLTLHH50 pKa = 6.09QKK52 pKa = 9.49VDD54 pKa = 3.41KK55 pKa = 9.84LTQNVKK61 pKa = 10.23QIQIKK66 pKa = 8.65EE67 pKa = 4.25AEE69 pKa = 4.17LSSVVKK75 pKa = 10.62NLQTKK80 pKa = 10.39NSTEE84 pKa = 3.95EE85 pKa = 4.02KK86 pKa = 9.74PVVKK90 pKa = 10.04TSPKK94 pKa = 10.0VFAVWEE100 pKa = 4.27DD101 pKa = 3.36PKK103 pKa = 11.3LQVEE107 pKa = 4.71AYY109 pKa = 8.31KK110 pKa = 10.42RR111 pKa = 11.84KK112 pKa = 10.06YY113 pKa = 8.02GQQVYY118 pKa = 9.31SHH120 pKa = 7.03TSHH123 pKa = 7.19KK124 pKa = 10.37GHH126 pKa = 6.62NN127 pKa = 3.64

Molecular weight:
14.63 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2368

127

1595

592.0

68.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.223 ± 0.755

1.858 ± 0.495

4.772 ± 0.644

7.855 ± 0.555

3.927 ± 0.312

4.307 ± 0.538

2.28 ± 0.529

8.488 ± 1.231

8.319 ± 1.283

10.346 ± 0.691

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.816 ± 0.251

5.659 ± 0.177

4.265 ± 0.838

5.954 ± 0.497

4.519 ± 0.531

6.25 ± 0.554

6.081 ± 0.8

4.223 ± 0.773

1.225 ± 0.253

3.632 ± 0.254

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski