Sapientia aquatica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Sapientia

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V3AUM5|A0A4V3AUM5_9BURK Peptidylprolyl isomerase OS=Sapientia aquatica OX=1549640 GN=E2I14_13060 PE=4 SV=1
MM1 pKa = 7.25QISTMGMFQNAVTSFNDD18 pKa = 3.53LQNNIAKK25 pKa = 8.98TINQVDD31 pKa = 3.57TGLALTSPADD41 pKa = 3.63NPAAASQVLVITQNKK56 pKa = 8.5NLNTQYY62 pKa = 11.82ALNGQNATNALSTADD77 pKa = 3.6GVLSGVSNLLQSLKK91 pKa = 9.95STIVQAGNGSLNASDD106 pKa = 4.67RR107 pKa = 11.84NTIVQQFQSGINQLLNLSNSTDD129 pKa = 3.21GNGNYY134 pKa = 9.83LFSGSATGSAGYY146 pKa = 10.1VATANGAQYY155 pKa = 10.98VGNQEE160 pKa = 4.1VQLLQVDD167 pKa = 4.65ASQQIAVTVPGNNVFGNIPVSANAFFGIPNANNTSTATISAGTVTNAASVTGDD220 pKa = 2.99NYY222 pKa = 11.27SINFTSATQYY232 pKa = 11.38DD233 pKa = 4.32VLDD236 pKa = 3.95TTTGATVASAQPYY249 pKa = 8.73TSGTAITFGGMQITITDD266 pKa = 3.98GASPTTGVPAAGDD279 pKa = 3.51KK280 pKa = 10.76FAVQPGNQNIFQALTNVITALKK302 pKa = 9.85SFSTATQGDD311 pKa = 3.85GTNFANALAQANTSIDD327 pKa = 3.35ASLSNVLTVRR337 pKa = 11.84DD338 pKa = 3.53QLGNSMQQVTSLKK351 pKa = 10.47LVSDD355 pKa = 4.13TTNISYY361 pKa = 7.65ATSLSSLQDD370 pKa = 3.14VNYY373 pKa = 10.48AQAISQLSLEE383 pKa = 4.2QFTYY387 pKa = 10.74QAAQKK392 pKa = 10.15AFAGTSQLSLISMLPVAA409 pKa = 5.4

Molecular weight: 42.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5VUH6|A0A4R5VUH6_9BURK N-acetyltransferase OS=Sapientia aquatica OX=1549640 GN=E2I14_15610 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3747

0

3747

1273207

29

5014

339.8

37.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.358 ± 0.043

0.912 ± 0.013

5.142 ± 0.029

5.2 ± 0.041

4.001 ± 0.024

7.127 ± 0.071

2.222 ± 0.022

6.199 ± 0.028

4.725 ± 0.041

10.259 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.428 ± 0.019

4.283 ± 0.053

4.426 ± 0.027

4.577 ± 0.027

4.871 ± 0.033

6.658 ± 0.046

5.707 ± 0.045

6.893 ± 0.031

1.276 ± 0.016

2.735 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski