Amino acid dipepetide frequency for Sapientia aquatica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.803AlaAla: 11.803 ± 0.146	0.982AlaCys: 0.982 ± 0.03	5.188AlaAsp: 5.188 ± 0.068	5.382AlaGlu: 5.382 ± 0.081	3.462AlaPhe: 3.462 ± 0.057	7.433AlaGly: 7.433 ± 0.1	2.224AlaHis: 2.224 ± 0.044	6.297AlaIle: 6.297 ± 0.084	5.108AlaLys: 5.108 ± 0.082	11.059AlaLeu: 11.059 ± 0.125	2.677AlaMet: 2.677 ± 0.043	4.351AlaAsn: 4.351 ± 0.072	4.394AlaPro: 4.394 ± 0.074	5.65AlaGln: 5.65 ± 0.083	4.87AlaArg: 4.87 ± 0.064	6.522AlaSer: 6.522 ± 0.097	5.852AlaThr: 5.852 ± 0.101	6.55AlaVal: 6.55 ± 0.087	1.223AlaTrp: 1.223 ± 0.032	2.557AlaTyr: 2.557 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.997CysAla: 0.997 ± 0.03	0.126CysCys: 0.126 ± 0.011	0.514CysAsp: 0.514 ± 0.019	0.463CysGlu: 0.463 ± 0.022	0.365CysPhe: 0.365 ± 0.019	0.845CysGly: 0.845 ± 0.027	0.251CysHis: 0.251 ± 0.017	0.495CysIle: 0.495 ± 0.018	0.363CysLys: 0.363 ± 0.016	0.883CysLeu: 0.883 ± 0.03	0.186CysMet: 0.186 ± 0.013	0.32CysAsn: 0.32 ± 0.016	0.412CysPro: 0.412 ± 0.019	0.348CysGln: 0.348 ± 0.016	0.425CysArg: 0.425 ± 0.018	0.607CysSer: 0.607 ± 0.023	0.472CysThr: 0.472 ± 0.021	0.662CysVal: 0.662 ± 0.024	0.137CysTrp: 0.137 ± 0.01	0.249CysTyr: 0.249 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	5.438AspAla: 5.438 ± 0.075	0.48AspCys: 0.48 ± 0.02	2.564AspAsp: 2.564 ± 0.049	2.967AspGlu: 2.967 ± 0.054	2.326AspPhe: 2.326 ± 0.04	3.83AspGly: 3.83 ± 0.064	1.127AspHis: 1.127 ± 0.034	3.164AspIle: 3.164 ± 0.054	2.493AspLys: 2.493 ± 0.045	5.302AspLeu: 5.302 ± 0.077	1.163AspMet: 1.163 ± 0.031	1.893AspAsn: 1.893 ± 0.051	2.319AspPro: 2.319 ± 0.04	2.414AspGln: 2.414 ± 0.044	2.268AspArg: 2.268 ± 0.043	2.976AspSer: 2.976 ± 0.052	2.428AspThr: 2.428 ± 0.048	3.738AspVal: 3.738 ± 0.055	0.847AspTrp: 0.847 ± 0.028	1.686AspTyr: 1.686 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	4.884GluAla: 4.884 ± 0.074	0.419GluCys: 0.419 ± 0.018	2.01GluAsp: 2.01 ± 0.045	2.564GluGlu: 2.564 ± 0.058	2.238GluPhe: 2.238 ± 0.043	2.868GluGly: 2.868 ± 0.062	1.352GluHis: 1.352 ± 0.039	3.657GluIle: 3.657 ± 0.063	2.847GluLys: 2.847 ± 0.049	6.156GluLeu: 6.156 ± 0.084	1.36GluMet: 1.36 ± 0.036	2.055GluAsn: 2.055 ± 0.043	1.87GluPro: 1.87 ± 0.045	3.333GluGln: 3.333 ± 0.087	3.278GluArg: 3.278 ± 0.057	2.83GluSer: 2.83 ± 0.049	2.646GluThr: 2.646 ± 0.055	3.623GluVal: 3.623 ± 0.062	0.704GluTrp: 0.704 ± 0.024	1.309GluTyr: 1.309 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.095PheAla: 4.095 ± 0.059	0.5PheCys: 0.5 ± 0.02	2.653PheAsp: 2.653 ± 0.043	2.062PheGlu: 2.062 ± 0.04	1.714PhePhe: 1.714 ± 0.049	3.384PheGly: 3.384 ± 0.065	0.804PheHis: 0.804 ± 0.026	2.36PheIle: 2.36 ± 0.05	1.871PheLys: 1.871 ± 0.042	3.285PheLeu: 3.285 ± 0.065	0.894PheMet: 0.894 ± 0.032	1.967PheAsn: 1.967 ± 0.047	1.613PhePro: 1.613 ± 0.037	1.297PheGln: 1.297 ± 0.035	1.566PheArg: 1.566 ± 0.035	3.134PheSer: 3.134 ± 0.053	2.241PheThr: 2.241 ± 0.047	2.777PheVal: 2.777 ± 0.054	0.532PheTrp: 0.532 ± 0.022	1.265PheTyr: 1.265 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	6.547GlyAla: 6.547 ± 0.093	0.771GlyCys: 0.771 ± 0.026	3.361GlyAsp: 3.361 ± 0.054	3.416GlyGlu: 3.416 ± 0.058	3.286GlyPhe: 3.286 ± 0.057	5.368GlyGly: 5.368 ± 0.133	1.571GlyHis: 1.571 ± 0.035	4.678GlyIle: 4.678 ± 0.069	3.916GlyLys: 3.916 ± 0.058	7.3GlyLeu: 7.3 ± 0.076	1.978GlyMet: 1.978 ± 0.04	3.03GlyAsn: 3.03 ± 0.203	1.955GlyPro: 1.955 ± 0.049	3.026GlyGln: 3.026 ± 0.06	3.347GlyArg: 3.347 ± 0.059	4.619GlySer: 4.619 ± 0.089	4.184GlyThr: 4.184 ± 0.137	5.484GlyVal: 5.484 ± 0.07	1.124GlyTrp: 1.124 ± 0.037	2.314GlyTyr: 2.314 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.224HisAla: 2.224 ± 0.042	0.26HisCys: 0.26 ± 0.015	1.184HisAsp: 1.184 ± 0.034	1.067HisGlu: 1.067 ± 0.029	1.014HisPhe: 1.014 ± 0.028	1.673HisGly: 1.673 ± 0.043	0.648HisHis: 0.648 ± 0.027	1.354HisIle: 1.354 ± 0.034	0.789HisLys: 0.789 ± 0.025	2.223HisLeu: 2.223 ± 0.043	0.498HisMet: 0.498 ± 0.019	0.79HisAsn: 0.79 ± 0.027	1.294HisPro: 1.294 ± 0.034	1.071HisGln: 1.071 ± 0.029	1.082HisArg: 1.082 ± 0.034	1.428HisSer: 1.428 ± 0.036	1.101HisThr: 1.101 ± 0.027	1.379HisVal: 1.379 ± 0.036	0.353HisTrp: 0.353 ± 0.015	0.785HisTyr: 0.785 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	7.208IleAla: 7.208 ± 0.085	0.585IleCys: 0.585 ± 0.021	3.899IleAsp: 3.899 ± 0.058	3.85IleGlu: 3.85 ± 0.066	2.126IlePhe: 2.126 ± 0.049	4.98IleGly: 4.98 ± 0.081	1.205IleHis: 1.205 ± 0.033	3.232IleIle: 3.232 ± 0.059	3.057IleLys: 3.057 ± 0.054	5.124IleLeu: 5.124 ± 0.079	1.146IleMet: 1.146 ± 0.029	3.006IleAsn: 3.006 ± 0.049	2.897IlePro: 2.897 ± 0.05	2.166IleGln: 2.166 ± 0.04	2.752IleArg: 2.752 ± 0.047	4.451IleSer: 4.451 ± 0.069	3.756IleThr: 3.756 ± 0.059	4.295IleVal: 4.295 ± 0.058	0.669IleTrp: 0.669 ± 0.026	1.584IleTyr: 1.584 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	4.424LysAla: 4.424 ± 0.073	0.236LysCys: 0.236 ± 0.014	2.182LysAsp: 2.182 ± 0.044	2.449LysGlu: 2.449 ± 0.049	1.673LysPhe: 1.673 ± 0.041	2.54LysGly: 2.54 ± 0.048	1.066LysHis: 1.066 ± 0.025	3.092LysIle: 3.092 ± 0.056	2.686LysLys: 2.686 ± 0.054	5.312LysLeu: 5.312 ± 0.071	1.361LysMet: 1.361 ± 0.035	2.306LysAsn: 2.306 ± 0.05	2.377LysPro: 2.377 ± 0.052	2.412LysGln: 2.412 ± 0.05	2.503LysArg: 2.503 ± 0.05	3.065LysSer: 3.065 ± 0.046	2.751LysThr: 2.751 ± 0.043	3.184LysVal: 3.184 ± 0.052	0.514LysTrp: 0.514 ± 0.023	1.114LysTyr: 1.114 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	11.403LeuAla: 11.403 ± 0.106	1.0LeuCys: 1.0 ± 0.028	5.456LeuAsp: 5.456 ± 0.08	5.057LeuGlu: 5.057 ± 0.079	4.025LeuPhe: 4.025 ± 0.071	7.169LeuGly: 7.169 ± 0.087	2.228LeuHis: 2.228 ± 0.048	6.424LeuIle: 6.424 ± 0.078	4.706LeuLys: 4.706 ± 0.067	10.169LeuLeu: 10.169 ± 0.131	2.403LeuMet: 2.403 ± 0.058	4.423LeuAsn: 4.423 ± 0.063	5.218LeuPro: 5.218 ± 0.072	4.072LeuGln: 4.072 ± 0.068	5.401LeuArg: 5.401 ± 0.082	7.374LeuSer: 7.374 ± 0.094	6.07LeuThr: 6.07 ± 0.077	6.674LeuVal: 6.674 ± 0.086	1.051LeuTrp: 1.051 ± 0.03	2.267LeuTyr: 2.267 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.35MetAla: 2.35 ± 0.053	0.177MetCys: 0.177 ± 0.011	1.141MetAsp: 1.141 ± 0.029	1.085MetGlu: 1.085 ± 0.025	0.828MetPhe: 0.828 ± 0.028	1.566MetGly: 1.566 ± 0.041	0.604MetHis: 0.604 ± 0.022	1.335MetIle: 1.335 ± 0.034	1.242MetLys: 1.242 ± 0.03	2.624MetLeu: 2.624 ± 0.047	0.681MetMet: 0.681 ± 0.028	1.102MetAsn: 1.102 ± 0.029	1.25MetPro: 1.25 ± 0.036	1.326MetGln: 1.326 ± 0.032	1.422MetArg: 1.422 ± 0.032	1.769MetSer: 1.769 ± 0.037	1.537MetThr: 1.537 ± 0.031	1.52MetVal: 1.52 ± 0.038	0.218MetTrp: 0.218 ± 0.012	0.507MetTyr: 0.507 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	4.354AsnAla: 4.354 ± 0.083	0.393AsnCys: 0.393 ± 0.018	2.073AsnAsp: 2.073 ± 0.039	1.908AsnGlu: 1.908 ± 0.04	1.626AsnPhe: 1.626 ± 0.034	3.456AsnGly: 3.456 ± 0.175	0.858AsnHis: 0.858 ± 0.027	2.39AsnIle: 2.39 ± 0.073	1.902AsnLys: 1.902 ± 0.045	4.272AsnLeu: 4.272 ± 0.065	0.939AsnMet: 0.939 ± 0.027	1.937AsnAsn: 1.937 ± 0.053	2.568AsnPro: 2.568 ± 0.048	1.968AsnGln: 1.968 ± 0.045	1.935AsnArg: 1.935 ± 0.038	2.995AsnSer: 2.995 ± 0.064	2.354AsnThr: 2.354 ± 0.086	2.84AsnVal: 2.84 ± 0.064	0.666AsnTrp: 0.666 ± 0.022	1.392AsnTyr: 1.392 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	4.674ProAla: 4.674 ± 0.083	0.313ProCys: 0.313 ± 0.018	2.735ProAsp: 2.735 ± 0.048	2.916ProGlu: 2.916 ± 0.052	1.767ProPhe: 1.767 ± 0.038	2.728ProGly: 2.728 ± 0.049	0.95ProHis: 0.95 ± 0.029	2.756ProIle: 2.756 ± 0.047	2.024ProLys: 2.024 ± 0.039	4.212ProLeu: 4.212 ± 0.061	1.052ProMet: 1.052 ± 0.029	2.101ProAsn: 2.101 ± 0.039	1.604ProPro: 1.604 ± 0.045	1.772ProGln: 1.772 ± 0.042	1.7ProArg: 1.7 ± 0.035	3.003ProSer: 3.003 ± 0.048	2.809ProThr: 2.809 ± 0.052	3.379ProVal: 3.379 ± 0.056	0.514ProTrp: 0.514 ± 0.02	1.246ProTyr: 1.246 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	4.927GlnAla: 4.927 ± 0.086	0.349GlnCys: 0.349 ± 0.016	1.722GlnAsp: 1.722 ± 0.033	2.136GlnGlu: 2.136 ± 0.05	1.975GlnPhe: 1.975 ± 0.044	2.773GlnGly: 2.773 ± 0.049	1.298GlnHis: 1.298 ± 0.037	3.077GlnIle: 3.077 ± 0.048	1.935GlnLys: 1.935 ± 0.042	5.255GlnLeu: 5.255 ± 0.09	1.051GlnMet: 1.051 ± 0.028	1.646GlnAsn: 1.646 ± 0.043	1.881GlnPro: 1.881 ± 0.042	2.92GlnGln: 2.92 ± 0.078	2.769GlnArg: 2.769 ± 0.058	2.694GlnSer: 2.694 ± 0.048	2.585GlnThr: 2.585 ± 0.046	3.09GlnVal: 3.09 ± 0.05	0.565GlnTrp: 0.565 ± 0.021	1.128GlnTyr: 1.128 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	4.744ArgAla: 4.744 ± 0.073	0.419ArgCys: 0.419 ± 0.019	2.629ArgAsp: 2.629 ± 0.048	2.846ArgGlu: 2.846 ± 0.06	2.265ArgPhe: 2.265 ± 0.047	2.929ArgGly: 2.929 ± 0.061	1.254ArgHis: 1.254 ± 0.035	3.333ArgIle: 3.333 ± 0.057	2.097ArgLys: 2.097 ± 0.043	5.222ArgLeu: 5.222 ± 0.076	1.337ArgMet: 1.337 ± 0.032	1.962ArgAsn: 1.962 ± 0.034	1.857ArgPro: 1.857 ± 0.043	2.286ArgGln: 2.286 ± 0.046	2.663ArgArg: 2.663 ± 0.059	2.961ArgSer: 2.961 ± 0.055	2.408ArgThr: 2.408 ± 0.042	3.203ArgVal: 3.203 ± 0.048	0.679ArgTrp: 0.679 ± 0.023	1.62ArgTyr: 1.62 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	7.198SerAla: 7.198 ± 0.085	0.53SerCys: 0.53 ± 0.02	3.527SerAsp: 3.527 ± 0.056	3.351SerGlu: 3.351 ± 0.054	2.687SerPhe: 2.687 ± 0.05	5.549SerGly: 5.549 ± 0.167	1.287SerHis: 1.287 ± 0.034	4.047SerIle: 4.047 ± 0.055	2.993SerLys: 2.993 ± 0.048	6.506SerLeu: 6.506 ± 0.074	1.601SerMet: 1.601 ± 0.035	3.012SerAsn: 3.012 ± 0.092	2.749SerPro: 2.749 ± 0.049	2.475SerGln: 2.475 ± 0.052	2.806SerArg: 2.806 ± 0.051	4.865SerSer: 4.865 ± 0.099	4.074SerThr: 4.074 ± 0.077	4.538SerVal: 4.538 ± 0.062	0.837SerTrp: 0.837 ± 0.027	1.945SerTyr: 1.945 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	5.459ThrAla: 5.459 ± 0.076	0.449ThrCys: 0.449 ± 0.02	2.865ThrAsp: 2.865 ± 0.053	2.881ThrGlu: 2.881 ± 0.06	2.046ThrPhe: 2.046 ± 0.04	4.391ThrGly: 4.391 ± 0.099	1.16ThrHis: 1.16 ± 0.034	3.693ThrIle: 3.693 ± 0.06	2.453ThrLys: 2.453 ± 0.043	6.052ThrLeu: 6.052 ± 0.074	1.253ThrMet: 1.253 ± 0.034	2.501ThrAsn: 2.501 ± 0.074	3.298ThrPro: 3.298 ± 0.055	2.448ThrGln: 2.448 ± 0.043	2.368ThrArg: 2.368 ± 0.044	3.96ThrSer: 3.96 ± 0.119	3.534ThrThr: 3.534 ± 0.077	4.013ThrVal: 4.013 ± 0.075	0.699ThrTrp: 0.699 ± 0.024	1.543ThrTyr: 1.543 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	7.329ValAla: 7.329 ± 0.077	0.652ValCys: 0.652 ± 0.023	3.768ValAsp: 3.768 ± 0.06	3.841ValGlu: 3.841 ± 0.06	2.643ValPhe: 2.643 ± 0.048	4.937ValGly: 4.937 ± 0.073	1.389ValHis: 1.389 ± 0.041	4.488ValIle: 4.488 ± 0.058	3.135ValLys: 3.135 ± 0.06	6.941ValLeu: 6.941 ± 0.088	1.778ValMet: 1.778 ± 0.04	2.858ValAsn: 2.858 ± 0.058	2.904ValPro: 2.904 ± 0.053	2.608ValGln: 2.608 ± 0.042	3.236ValArg: 3.236 ± 0.052	4.546ValSer: 4.546 ± 0.063	4.212ValThr: 4.212 ± 0.078	5.286ValVal: 5.286 ± 0.074	0.787ValTrp: 0.787 ± 0.025	1.59ValTyr: 1.59 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	0.983TrpAla: 0.983 ± 0.032	0.148TrpCys: 0.148 ± 0.01	0.606TrpAsp: 0.606 ± 0.025	0.54TrpGlu: 0.54 ± 0.021	0.557TrpPhe: 0.557 ± 0.021	0.781TrpGly: 0.781 ± 0.025	0.36TrpHis: 0.36 ± 0.016	0.814TrpIle: 0.814 ± 0.026	0.572TrpLys: 0.572 ± 0.024	1.645TrpLeu: 1.645 ± 0.044	0.419TrpMet: 0.419 ± 0.019	0.562TrpAsn: 0.562 ± 0.023	0.462TrpPro: 0.462 ± 0.019	0.763TrpGln: 0.763 ± 0.026	0.781TrpArg: 0.781 ± 0.031	0.767TrpSer: 0.767 ± 0.023	0.617TrpThr: 0.617 ± 0.024	0.844TrpVal: 0.844 ± 0.027	0.193TrpTrp: 0.193 ± 0.015	0.344TrpTyr: 0.344 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.541TyrAla: 2.541 ± 0.05	0.329TyrCys: 0.329 ± 0.019	1.346TyrAsp: 1.346 ± 0.037	1.224TyrGlu: 1.224 ± 0.029	1.393TyrPhe: 1.393 ± 0.038	2.074TyrGly: 2.074 ± 0.045	0.583TyrHis: 0.583 ± 0.018	1.301TyrIle: 1.301 ± 0.033	1.059TyrLys: 1.059 ± 0.028	3.048TyrLeu: 3.048 ± 0.054	0.506TyrMet: 0.506 ± 0.02	1.003TyrAsn: 1.003 ± 0.033	1.333TyrPro: 1.333 ± 0.037	1.419TyrGln: 1.419 ± 0.034	1.542TyrArg: 1.542 ± 0.039	2.011TyrSer: 2.011 ± 0.045	1.437TyrThr: 1.437 ± 0.039	1.85TyrVal: 1.85 ± 0.039	0.447TyrTrp: 0.447 ± 0.021	0.905TyrTyr: 0.905 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3747 proteins (1273207 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---