Collinsella tanakaei YIT 12063
Average proteome isoelectric point is 5.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2204 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1WJB8|G1WJB8_9ACTN 50S ribosomal protein L5 OS=Collinsella tanakaei YIT 12063 OX=742742 GN=rplE PE=3 SV=1
MM1 pKa = 7.55 AVNEE5 pKa = 4.0 QLLLEE10 pKa = 4.26 VLEE13 pKa = 4.33 QIRR16 pKa = 11.84 PNLQADD22 pKa = 4.42 GGDD25 pKa = 3.4 MAYY28 pKa = 10.73 VGVDD32 pKa = 3.23 DD33 pKa = 5.69 DD34 pKa = 4.93 GVVSLEE40 pKa = 4.16 LQGACAGCPMSQLTLSMGVEE60 pKa = 4.72 RR61 pKa = 11.84 ILKK64 pKa = 8.09 EE65 pKa = 3.75 HH66 pKa = 6.12 VPGVTRR72 pKa = 11.84 VEE74 pKa = 4.15 AVNNGPAADD83 pKa = 4.52 MYY85 pKa = 11.49 DD86 pKa = 3.52 EE87 pKa = 4.36 YY88 pKa = 11.79 APFF91 pKa = 4.6
Molecular weight: 9.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.948
IPC_protein 3.846
Toseland 3.656
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.681
Grimsley 3.579
Solomon 3.795
Lehninger 3.757
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.961
Patrickios 1.888
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|G1WL11|G1WL11_9ACTN SHSP domain-containing protein OS=Collinsella tanakaei YIT 12063 OX=742742 GN=HMPREF9452_02024 PE=3 SV=1
MM1 pKa = 7.45 RR2 pKa = 11.84 TLKK5 pKa = 10.67 SRR7 pKa = 11.84 LEE9 pKa = 3.87 FEE11 pKa = 4.32 RR12 pKa = 11.84 AFTQGRR18 pKa = 11.84 RR19 pKa = 11.84 YY20 pKa = 8.72 NHH22 pKa = 6.34 PLIRR26 pKa = 11.84 MVICDD31 pKa = 4.29 AVNEE35 pKa = 4.18 GDD37 pKa = 3.65 PGRR40 pKa = 11.84 VAFVAAKK47 pKa = 10.26 RR48 pKa = 11.84 LGCAVVRR55 pKa = 11.84 NRR57 pKa = 11.84 SKK59 pKa = 10.82 RR60 pKa = 11.84 VLRR63 pKa = 11.84 EE64 pKa = 3.2 AARR67 pKa = 11.84 AVKK70 pKa = 10.43 LPVDD74 pKa = 4.56 GYY76 pKa = 11.22 DD77 pKa = 3.36 IILFATPRR85 pKa = 11.84 TRR87 pKa = 11.84 DD88 pKa = 3.2 SSPQQMIEE96 pKa = 4.16 ALCKK100 pKa = 9.46 LCEE103 pKa = 4.01 RR104 pKa = 11.84 ADD106 pKa = 3.48 LHH108 pKa = 7.12 VSVRR112 pKa = 3.57
Molecular weight: 12.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.399
IPC_protein 10.058
Toseland 10.511
ProMoST 10.482
Dawson 10.613
Bjellqvist 10.335
Wikipedia 10.818
Rodwell 10.76
Grimsley 10.657
Solomon 10.745
Lehninger 10.716
Nozaki 10.555
DTASelect 10.321
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.555
Patrickios 10.526
IPC_peptide 10.745
IPC2_peptide 9.648
IPC2.peptide.svr19 8.605
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2204
0
2204
707062
29
2459
320.8
34.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.662 ± 0.081
1.554 ± 0.022
6.435 ± 0.046
6.251 ± 0.056
3.629 ± 0.032
8.145 ± 0.057
1.883 ± 0.026
5.314 ± 0.044
3.74 ± 0.048
9.099 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.811 ± 0.026
2.982 ± 0.039
4.238 ± 0.034
3.033 ± 0.029
6.012 ± 0.058
6.171 ± 0.049
5.24 ± 0.04
7.959 ± 0.045
1.012 ± 0.018
2.829 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here