Dokdonella koreensis DS-123
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3774 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160DXW4|A0A160DXW4_9GAMM Uncharacterized protein OS=Dokdonella koreensis DS-123 OX=1300342 GN=I596_3599 PE=4 SV=1
MM1 pKa = 7.39 TMQSRR6 pKa = 11.84 AARR9 pKa = 11.84 AAVSLCVMVVALAAGALGAQPATGPLQFSAPVSAPFVEE47 pKa = 4.87 TKK49 pKa = 10.4 PVVANPFSEE58 pKa = 4.34 AARR61 pKa = 11.84 PVSPRR66 pKa = 11.84 AFSTEE71 pKa = 3.73 FPAEE75 pKa = 4.03 VEE77 pKa = 4.39 PNGTPATATPLGAATYY93 pKa = 11.27 VRR95 pKa = 11.84 MQGNLFPAADD105 pKa = 3.25 VDD107 pKa = 4.31 YY108 pKa = 11.09 FSFAAQAGDD117 pKa = 4.06 RR118 pKa = 11.84 IYY120 pKa = 11.3 AAVITAANTSTSADD134 pKa = 3.53 SQLNLLDD141 pKa = 3.82 TDD143 pKa = 3.78 GTTVLEE149 pKa = 4.51 FDD151 pKa = 5.18 DD152 pKa = 6.03 DD153 pKa = 4.66 DD154 pKa = 5.92 GSFAATSSSIAGTVIPADD172 pKa = 3.28 GTYY175 pKa = 10.21 YY176 pKa = 10.97 LSVNHH181 pKa = 5.78 FTAAQTLRR189 pKa = 11.84 PYY191 pKa = 9.28 EE192 pKa = 3.78 LHH194 pKa = 6.69 VRR196 pKa = 11.84 IQRR199 pKa = 11.84 GSPTPEE205 pKa = 3.74 VEE207 pKa = 4.98 SNDD210 pKa = 3.75 TPGTANPLPANGWVSGTRR228 pKa = 11.84 NPPAATEE235 pKa = 3.98 QDD237 pKa = 3.29 WYY239 pKa = 11.18 AFTANAGDD247 pKa = 4.02 TVFLSLDD254 pKa = 3.77 ADD256 pKa = 3.89 PEE258 pKa = 4.32 RR259 pKa = 11.84 DD260 pKa = 3.4 GVQWDD265 pKa = 3.61 GRR267 pKa = 11.84 LGIALFGDD275 pKa = 4.35 AANQILVVNDD285 pKa = 3.69 TSSGAGNPLSEE296 pKa = 4.46 ALVFTIKK303 pKa = 9.79 TAGTYY308 pKa = 9.75 FAFVDD313 pKa = 3.85 SATAAVGGPTATYY326 pKa = 8.87 HH327 pKa = 6.62 LSVSVQPPPTPMPLAACTTYY347 pKa = 10.85 TSTDD351 pKa = 3.19 VPKK354 pKa = 10.5 TIGPANGLVSSLITVPGNPRR374 pKa = 11.84 IGDD377 pKa = 3.93 LNVEE381 pKa = 3.99 IQLNHH386 pKa = 6.73 ALMADD391 pKa = 3.52 VDD393 pKa = 3.68 AHH395 pKa = 6.32 LRR397 pKa = 11.84 SPSGNDD403 pKa = 2.46 IGLFTDD409 pKa = 3.65 IGAAATGGQAQMDD422 pKa = 4.0 VVFDD426 pKa = 4.49 DD427 pKa = 4.58 EE428 pKa = 4.87 AAIPPTFTALRR439 pKa = 11.84 NMALKK444 pKa = 10.66 PEE446 pKa = 4.26 LNYY449 pKa = 10.57 RR450 pKa = 11.84 LSWFDD455 pKa = 3.41 GEE457 pKa = 4.52 NAGGTWTLDD466 pKa = 3.94 LRR468 pKa = 11.84 DD469 pKa = 4.39 DD470 pKa = 4.06 VNNASGGTLTSWSLRR485 pKa = 11.84 ICEE488 pKa = 4.53 PPPPPTCGPGFVQTTVYY505 pKa = 9.77 STDD508 pKa = 3.92 FEE510 pKa = 4.57 SGAAGFTHH518 pKa = 7.23 SGTQDD523 pKa = 2.74 EE524 pKa = 4.98 WEE526 pKa = 4.66 LGLPATVATTTANPVAAFNSCASGVNCWKK555 pKa = 9.61 TDD557 pKa = 3.3 LDD559 pKa = 3.48 NTYY562 pKa = 10.8 NISSNQNLTSPSINLGGFSPPIVVRR587 pKa = 11.84 WSHH590 pKa = 5.1 QYY592 pKa = 10.56 QMEE595 pKa = 4.34 SASFDD600 pKa = 3.92 LYY602 pKa = 11.36 SVDD605 pKa = 3.36 LRR607 pKa = 11.84 EE608 pKa = 4.21 VGGANPVRR616 pKa = 11.84 LFEE619 pKa = 4.35 WLDD622 pKa = 3.3 ATMTDD627 pKa = 3.2 AVGNPTVNIPARR639 pKa = 11.84 AGWSQVVTRR648 pKa = 11.84 ADD650 pKa = 3.17 ALAGQNVEE658 pKa = 4.9 LNFNLTSDD666 pKa = 3.38 NTVNFGGVAVDD677 pKa = 4.07 DD678 pKa = 4.53 VSVTACRR685 pKa = 11.84 VAIADD690 pKa = 4.0 LSITKK695 pKa = 9.61 TDD697 pKa = 3.45 GVTTAVPGGSVTYY710 pKa = 10.02 TITASNAGPDD720 pKa = 3.77 ADD722 pKa = 4.15 TAATVADD729 pKa = 4.52 TFPAALTCTWTCVGAGGGTCTAAGSGNIADD759 pKa = 4.33 TVNLPSGGSVTYY771 pKa = 8.84 TASCAIGPSVTGTLSNTATVTASVADD797 pKa = 4.17 PNPANNSATDD807 pKa = 3.63 TDD809 pKa = 4.28 TLTPQADD816 pKa = 3.73 LAITKK821 pKa = 9.28 TDD823 pKa = 3.06 GVTSVIQGGSTTYY836 pKa = 10.62 TITASNAGPSNASGATVTDD855 pKa = 4.01 TFPASLTCTWTCVGAGGGTCTAAGSGNIADD885 pKa = 4.5 TVNLPAGGSTTHH897 pKa = 6.41 TASCGLSASATGTLSNTATVAAPAGVTDD925 pKa = 4.31 PTPGNNSATDD935 pKa = 3.41 TDD937 pKa = 4.55 TITLLGAAVTASKK950 pKa = 9.37 TVAGSFVPGSPVTYY964 pKa = 9.69 TVTLTNSLGAQANNPGDD981 pKa = 3.91 EE982 pKa = 4.4 FTDD985 pKa = 3.6 VLPAALTLVSATATSGSAVATIGTNTVTWNGAIPGGGSVTITITATLSPSATGTVANQGSFAYY1048 pKa = 10.4 DD1049 pKa = 3.17 GDD1051 pKa = 4.5 ANGSNEE1057 pKa = 4.4 TNGLTDD1063 pKa = 5.48 DD1064 pKa = 4.44 PAQPGAADD1072 pKa = 3.43 PTVFTVGAAVADD1084 pKa = 4.02 LAITKK1089 pKa = 9.57 TDD1091 pKa = 3.23 GVTTATPGGSTTYY1104 pKa = 10.45 TIVASNAGPSAVTGATVTDD1123 pKa = 4.14 TFPASLTCTWTCAGSGGGTCAPSGSGNIASPVTLPVGASATYY1165 pKa = 8.69 TATCAISATASGTLVNTATIAVPAGVNDD1193 pKa = 3.85 PTPGNNSATDD1203 pKa = 3.72 TDD1205 pKa = 4.25 TLAATADD1212 pKa = 3.86 LAITKK1217 pKa = 9.51 TDD1219 pKa = 3.38 GVTSATPGDD1228 pKa = 3.83 STTYY1232 pKa = 10.41 TIVASNAGPSAVTGATVTDD1251 pKa = 3.96 TFAASLTCTWTCAGSGGGTCAPSGSGNIASPVTLPVGASATYY1293 pKa = 8.69 TATCAISATASGTLVNTATIAAPAGVTDD1321 pKa = 4.31 PTPGNNSATDD1331 pKa = 3.52 TDD1333 pKa = 3.84 ALGAEE1338 pKa = 4.41 TALSISKK1345 pKa = 9.21 TDD1347 pKa = 3.12 GATTVAPGGTLTYY1360 pKa = 10.01 TIVASNVGPSAAVGATVSDD1379 pKa = 4.59 AFPAACVAPAWTCAASGGGNCPAGGSGAIAASVDD1413 pKa = 4.05 LPAGAAATFTATCPVDD1429 pKa = 3.22 PALADD1434 pKa = 3.42 GTVITNTATVAEE1446 pKa = 4.47 AAGATDD1452 pKa = 5.28 PDD1454 pKa = 4.41 PSDD1457 pKa = 3.98 NSATDD1462 pKa = 3.35 TTTAAVPVAGAAVSVRR1478 pKa = 11.84 KK1479 pKa = 9.67 SVSGSFLPGGAIRR1492 pKa = 11.84 YY1493 pKa = 6.61 TLVLVNSGGPQGDD1506 pKa = 3.65 NPGDD1510 pKa = 3.81 EE1511 pKa = 4.79 LTDD1514 pKa = 3.46 VLPPQLVLVAASASSGAASANPATNTVTWNGAIPAGGSVTVLIDD1558 pKa = 3.07 ATIRR1562 pKa = 11.84 SDD1564 pKa = 3.46 ASGTIGNQAGVAYY1577 pKa = 10.33 DD1578 pKa = 3.73 GDD1580 pKa = 4.13 GDD1582 pKa = 4.14 GSNEE1586 pKa = 3.7 ATALSDD1592 pKa = 3.87 DD1593 pKa = 4.09 PAVPGAADD1601 pKa = 3.37 PTVFPVGAPAVVVPVPTLTGLGALLLGLGLAGLAIAARR1639 pKa = 11.84 PRR1641 pKa = 11.84 RR1642 pKa = 11.84 RR1643 pKa = 11.84 LRR1645 pKa = 3.48
Molecular weight: 161.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.719
IPC_protein 3.795
Toseland 3.541
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.757
Rodwell 3.605
Grimsley 3.452
Solomon 3.795
Lehninger 3.757
Nozaki 3.897
DTASelect 4.215
Thurlkill 3.605
EMBOSS 3.757
Sillero 3.91
Patrickios 0.769
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A167H409|A0A167H409_9GAMM Uncharacterized protein OS=Dokdonella koreensis DS-123 OX=1300342 GN=I596_2808 PE=4 SV=1
MM1 pKa = 7.15 GHH3 pKa = 6.7 RR4 pKa = 11.84 GVVFWQRR11 pKa = 11.84 KK12 pKa = 4.67 VRR14 pKa = 11.84 GIVPGSAVRR23 pKa = 11.84 AGSGRR28 pKa = 11.84 FITGAQRR35 pKa = 11.84 VPAAARR41 pKa = 11.84 VLRR44 pKa = 11.84 AA45 pKa = 3.48
Molecular weight: 4.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.489
IPC2_protein 11.082
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.398
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.149
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3774
0
3774
1322314
37
2826
350.4
37.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.354 ± 0.071
0.855 ± 0.013
5.922 ± 0.028
4.905 ± 0.042
3.263 ± 0.024
9.031 ± 0.048
2.071 ± 0.021
4.079 ± 0.028
2.001 ± 0.032
10.811 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.707 ± 0.017
2.267 ± 0.028
5.772 ± 0.028
3.293 ± 0.023
8.218 ± 0.054
4.858 ± 0.043
5.305 ± 0.058
7.521 ± 0.037
1.496 ± 0.018
2.271 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here