Simian mastadenovirus C
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M9YVL4|M9YVL4_9ADEN E4 ORF1 OS=Simian mastadenovirus C OX=1962300 PE=4 SV=1
MM1 pKa = 7.52 RR2 pKa = 11.84 LVPEE6 pKa = 4.41 MYY8 pKa = 10.14 GVSWDD13 pKa = 3.59 EE14 pKa = 4.01 TAEE17 pKa = 3.96 EE18 pKa = 4.23 LLNAEE23 pKa = 4.83 IYY25 pKa = 10.11 DD26 pKa = 4.05 VPNLPPGTPSLHH38 pKa = 7.41 DD39 pKa = 4.21 LFDD42 pKa = 3.62 VEE44 pKa = 5.13 NDD46 pKa = 3.38 GGQDD50 pKa = 3.47 EE51 pKa = 5.45 NEE53 pKa = 4.19 DD54 pKa = 3.86 AVNSMFPDD62 pKa = 3.36 SMLSAGEE69 pKa = 4.74 GYY71 pKa = 10.83 AGDD74 pKa = 4.04 VDD76 pKa = 4.34 PSGSDD81 pKa = 3.15 MDD83 pKa = 4.33 LKK85 pKa = 10.81 CYY87 pKa = 10.45 EE88 pKa = 5.14 DD89 pKa = 4.41 GLPSSSSEE97 pKa = 3.88 GSDD100 pKa = 3.22 EE101 pKa = 5.93 DD102 pKa = 3.53 EE103 pKa = 4.28 QKK105 pKa = 10.6 PLKK108 pKa = 10.43 HH109 pKa = 6.36 EE110 pKa = 4.44 LVLDD114 pKa = 3.97 CPKK117 pKa = 11.0 NPGHH121 pKa = 7.25 DD122 pKa = 3.36 CRR124 pKa = 11.84 ACAFHH129 pKa = 7.29 RR130 pKa = 11.84 ATSGNTEE137 pKa = 4.86 AICCLCYY144 pKa = 9.94 MRR146 pKa = 11.84 LTSDD150 pKa = 3.87 FVYY153 pKa = 10.99 SDD155 pKa = 3.48 VSDD158 pKa = 3.93 VEE160 pKa = 4.2 GDD162 pKa = 3.42 GDD164 pKa = 3.59 KK165 pKa = 11.38 SKK167 pKa = 11.04 VSEE170 pKa = 4.46 SPGSLGTVVPDD181 pKa = 3.89 GVLKK185 pKa = 8.9 PTAVRR190 pKa = 11.84 VSARR194 pKa = 11.84 RR195 pKa = 11.84 RR196 pKa = 11.84 QAVEE200 pKa = 3.77 KK201 pKa = 11.15 LEE203 pKa = 5.37 DD204 pKa = 3.72 LLQEE208 pKa = 4.36 PEE210 pKa = 3.97 QTEE213 pKa = 4.14 PLDD216 pKa = 4.63 LSLKK220 pKa = 8.2 QPRR223 pKa = 11.84 MTT225 pKa = 4.26
Molecular weight: 24.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.16
IPC2_protein 4.075
IPC_protein 4.062
Toseland 3.859
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.177
Wikipedia 3.935
Rodwell 3.884
Grimsley 3.77
Solomon 4.024
Lehninger 3.986
Nozaki 4.139
DTASelect 4.342
Thurlkill 3.897
EMBOSS 3.948
Sillero 4.177
Patrickios 3.35
IPC_peptide 4.024
IPC2_peptide 4.164
IPC2.peptide.svr19 4.082
Protein with the highest isoelectric point:
>tr|M9YVE2|M9YVE2_9ADEN Shutoff protein OS=Simian mastadenovirus C OX=1962300 GN=L4 PE=2 SV=1
MM1 pKa = 7.74 ALTCRR6 pKa = 11.84 VRR8 pKa = 11.84 IPVPHH13 pKa = 5.34 YY14 pKa = 9.86 RR15 pKa = 11.84 GRR17 pKa = 11.84 TRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GMAGSGRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 ALRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 MKK38 pKa = 10.81 GGILPALIPIIAAAIGAIPGIASVAVQASRR68 pKa = 11.84 KK69 pKa = 9.21
Molecular weight: 7.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.397
IPC2_protein 10.994
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.164
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
31
5
36
11419
53
1185
317.2
35.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.636 ± 0.447
1.988 ± 0.312
4.773 ± 0.248
6.078 ± 0.555
3.967 ± 0.236
5.99 ± 0.263
2.154 ± 0.233
3.862 ± 0.198
3.766 ± 0.331
9.87 ± 0.539
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.391 ± 0.165
4.685 ± 0.498
6.612 ± 0.311
4.256 ± 0.236
7.444 ± 0.591
7.102 ± 0.363
5.771 ± 0.326
6.769 ± 0.351
1.322 ± 0.109
3.564 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here