Betaproteobacteria bacterium GR16-43

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; unclassified Betaproteobacteria

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8FFF4|A0A1P8FFF4_9PROT Outer-membrane lipoprotein LolB OS=Betaproteobacteria bacterium GR16-43 OX=1904640 GN=BWI17_03295 PE=3 SV=1
MM1 pKa = 7.57NDD3 pKa = 3.32MATVIPDD10 pKa = 3.66PLVFTEE16 pKa = 4.27SAANKK21 pKa = 9.2VKK23 pKa = 10.78ALIEE27 pKa = 4.07EE28 pKa = 4.41EE29 pKa = 4.51GNNEE33 pKa = 3.49LKK35 pKa = 10.93LRR37 pKa = 11.84VFVTGGGCSGLQYY50 pKa = 11.18GFTFDD55 pKa = 4.98EE56 pKa = 4.64ITNEE60 pKa = 4.06DD61 pKa = 3.94DD62 pKa = 3.62TVMSKK67 pKa = 10.89NGVSLLIDD75 pKa = 3.63PMSYY79 pKa = 10.43QYY81 pKa = 10.51LTGAEE86 pKa = 3.66IDD88 pKa = 3.78YY89 pKa = 10.76TEE91 pKa = 4.44GLEE94 pKa = 4.23GAQFVIKK101 pKa = 10.5NPNASSTCGCGSSFSVV117 pKa = 3.54

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8FGK4|A0A1P8FGK4_9PROT Guanylate cyclase domain-containing protein OS=Betaproteobacteria bacterium GR16-43 OX=1904640 GN=BWI17_05410 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSSAHH11 pKa = 5.91RR12 pKa = 11.84KK13 pKa = 6.42KK14 pKa = 8.64THH16 pKa = 5.21GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTASGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.32GRR39 pKa = 11.84KK40 pKa = 8.57RR41 pKa = 11.84VAVV44 pKa = 3.64

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4404

0

4404

1490001

37

9266

338.3

36.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.739 ± 0.067

0.844 ± 0.014

5.249 ± 0.029

5.559 ± 0.047

3.838 ± 0.025

8.619 ± 0.043

1.989 ± 0.024

4.487 ± 0.025

3.86 ± 0.035

9.818 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.018

2.814 ± 0.036

5.342 ± 0.025

2.995 ± 0.017

6.952 ± 0.052

5.352 ± 0.031

5.549 ± 0.082

7.93 ± 0.03

1.399 ± 0.019

2.363 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski