Ruminococcus sp. 1xD21-23

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; unclassified Ruminococcus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7725 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9E0D3|A0A3A9E0D3_9FIRM Thymidylate synthase OS=Ruminococcus sp. 1xD21-23 OX=2320089 GN=D7W80_21115 PE=4 SV=1
MM1 pKa = 6.94MVAALGLGGCAGGDD15 pKa = 3.45GAQEE19 pKa = 4.07NGSVSDD25 pKa = 3.93AGAGGGSSAAKK36 pKa = 8.45TQGAEE41 pKa = 4.13PAGEE45 pKa = 4.02PAGEE49 pKa = 4.01AVTLQIFAANSLTKK63 pKa = 10.85AMAEE67 pKa = 3.93AQEE70 pKa = 6.0LYY72 pKa = 9.15TQQNPNVTFGDD83 pKa = 3.78TQYY86 pKa = 10.95EE87 pKa = 4.01ASGTLNEE94 pKa = 4.37MLGAGQYY101 pKa = 11.39ADD103 pKa = 3.57ILITASKK110 pKa = 9.43GTMDD114 pKa = 4.34DD115 pKa = 4.05AVDD118 pKa = 3.27AGYY121 pKa = 11.01VDD123 pKa = 3.9EE124 pKa = 4.76ATRR127 pKa = 11.84STMFTNQLVFVTKK140 pKa = 10.51EE141 pKa = 3.85GGEE144 pKa = 4.06LADD147 pKa = 3.73TDD149 pKa = 3.88VTLEE153 pKa = 4.02DD154 pKa = 3.93VAAGTYY160 pKa = 7.64TLSVGDD166 pKa = 4.1EE167 pKa = 4.02NVPAGNYY174 pKa = 9.21ACQALSTVGAYY185 pKa = 9.38IEE187 pKa = 4.61PDD189 pKa = 3.34GATGADD195 pKa = 3.22ATGKK199 pKa = 10.63GGEE202 pKa = 4.34FSDD205 pKa = 3.74ALKK208 pKa = 11.11DD209 pKa = 3.66KK210 pKa = 9.75VTLGGKK216 pKa = 9.65VGDD219 pKa = 3.59VCKK222 pKa = 10.52YY223 pKa = 11.13AEE225 pKa = 4.46TGDD228 pKa = 3.33VDD230 pKa = 4.23LAMVYY235 pKa = 10.07TSDD238 pKa = 3.64VYY240 pKa = 11.48RR241 pKa = 11.84MGGVTICSVVPDD253 pKa = 4.39DD254 pKa = 3.41THH256 pKa = 8.21KK257 pKa = 11.18AITYY261 pKa = 8.23PGAVTAQSEE270 pKa = 4.15NAEE273 pKa = 3.95AAKK276 pKa = 10.7AFLEE280 pKa = 4.11WCMTDD285 pKa = 3.5EE286 pKa = 4.87DD287 pKa = 5.05CAKK290 pKa = 9.96IWQEE294 pKa = 3.59WGFDD298 pKa = 3.46MATEE302 pKa = 4.16

Molecular weight: 30.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9E1T4|A0A3A9E1T4_9FIRM Fibronectin type III domain-containing protein OS=Ruminococcus sp. 1xD21-23 OX=2320089 GN=D7W80_26235 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.63NRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.89VHH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.77VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.43GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7725

0

7725

2202755

17

4467

285.1

31.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.044 ± 0.047

1.548 ± 0.014

5.643 ± 0.029

7.442 ± 0.034

4.037 ± 0.023

7.689 ± 0.031

1.762 ± 0.012

6.14 ± 0.031

5.657 ± 0.032

9.091 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.95 ± 0.017

3.771 ± 0.023

3.787 ± 0.021

3.288 ± 0.019

5.286 ± 0.025

5.664 ± 0.022

5.166 ± 0.023

7.136 ± 0.029

1.146 ± 0.01

3.752 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski