Acetobacteroides hydrogenigenes
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3211 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R2EIB1|A0A4R2EIB1_9BACT Protein-export membrane protein SecG OS=Acetobacteroides hydrogenigenes OX=979970 GN=CLV25_11454 PE=3 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.11 LASKK10 pKa = 11.0 LEE12 pKa = 4.24 DD13 pKa = 4.09 APWPASKK20 pKa = 11.07 EE21 pKa = 3.94 EE22 pKa = 4.66 LIDD25 pKa = 3.41 YY26 pKa = 7.58 ATRR29 pKa = 11.84 SGAPLEE35 pKa = 4.43 VIEE38 pKa = 4.42 NLQEE42 pKa = 4.61 IEE44 pKa = 4.57 DD45 pKa = 3.67 EE46 pKa = 4.53 GEE48 pKa = 3.89 IYY50 pKa = 10.67 EE51 pKa = 4.65 SIEE54 pKa = 4.99 DD55 pKa = 3.43 IWPDD59 pKa = 3.76 YY60 pKa = 10.16 PSKK63 pKa = 11.32 DD64 pKa = 2.97 DD65 pKa = 3.97 FFFNEE70 pKa = 4.51 DD71 pKa = 3.17 EE72 pKa = 4.46 YY73 pKa = 11.99
Molecular weight: 8.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.848
IPC2_protein 3.617
IPC_protein 3.541
Toseland 3.363
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.427
Rodwell 3.376
Grimsley 3.274
Solomon 3.49
Lehninger 3.439
Nozaki 3.656
DTASelect 3.77
Thurlkill 3.414
EMBOSS 3.439
Sillero 3.656
Patrickios 1.761
IPC_peptide 3.49
IPC2_peptide 3.63
IPC2.peptide.svr19 3.675
Protein with the highest isoelectric point:
>tr|A0A4R2E3A0|A0A4R2E3A0_9BACT Tetratricopeptide repeat protein OS=Acetobacteroides hydrogenigenes OX=979970 GN=CLV25_1226 PE=4 SV=1
MM1 pKa = 7.68 LNRR4 pKa = 11.84 VSLQRR9 pKa = 11.84 TVRR12 pKa = 11.84 NSFFMRR18 pKa = 11.84 GNHH21 pKa = 5.89 FSTFGSLFLNRR32 pKa = 11.84 GSHH35 pKa = 7.15 LLMLGNHH42 pKa = 5.67 FFIRR46 pKa = 11.84 GNHH49 pKa = 5.53 FPTSGNHH56 pKa = 5.51 FLMRR60 pKa = 11.84 GNHH63 pKa = 6.0 FLTSRR68 pKa = 11.84 NHH70 pKa = 5.93 FLMRR74 pKa = 11.84 GNHH77 pKa = 5.58 FPTFRR82 pKa = 11.84 SLFLKK87 pKa = 10.47 RR88 pKa = 11.84 GSHH91 pKa = 6.99 LLMLGNHH98 pKa = 5.96 FLIRR102 pKa = 11.84 GNHH105 pKa = 5.51 FPTSGNHH112 pKa = 5.1 FLMRR116 pKa = 11.84 GSHH119 pKa = 6.89 FLTFGNHH126 pKa = 5.39 FLMRR130 pKa = 11.84 GNHH133 pKa = 6.25 FLTSGNRR140 pKa = 11.84 FLRR143 pKa = 11.84 RR144 pKa = 11.84 GAKK147 pKa = 8.01 TT148 pKa = 3.08
Molecular weight: 17.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.433
IPC_protein 13.042
Toseland 13.203
ProMoST 13.7
Dawson 13.203
Bjellqvist 13.203
Wikipedia 13.685
Rodwell 12.749
Grimsley 13.247
Solomon 13.7
Lehninger 13.598
Nozaki 13.203
DTASelect 13.203
Thurlkill 13.203
EMBOSS 13.7
Sillero 13.203
Patrickios 12.486
IPC_peptide 13.715
IPC2_peptide 12.691
IPC2.peptide.svr19 9.331
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3211
0
3211
1096128
29
3629
341.4
38.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.509 ± 0.037
0.954 ± 0.014
5.186 ± 0.028
6.115 ± 0.05
4.762 ± 0.028
6.878 ± 0.044
1.663 ± 0.017
7.168 ± 0.039
7.096 ± 0.038
9.235 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.493 ± 0.022
5.203 ± 0.042
3.684 ± 0.023
3.232 ± 0.023
4.271 ± 0.033
6.861 ± 0.045
5.54 ± 0.057
6.991 ± 0.038
1.079 ± 0.017
4.079 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here