Jeotgalibaca porci
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2201 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G7WF22|A0A6G7WF22_9LACT Flotillin family protein OS=Jeotgalibaca porci OX=1868793 GN=G7058_01520 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.46 KK3 pKa = 10.23 SVLSLLSTVSLLTLLTACGGGEE25 pKa = 4.14 TEE27 pKa = 4.36 EE28 pKa = 4.3 QEE30 pKa = 4.63 SVTRR34 pKa = 11.84 TDD36 pKa = 3.1 GTTEE40 pKa = 3.75 TSEE43 pKa = 4.32 TADD46 pKa = 3.44 STEE49 pKa = 4.14 TASGGSVYY57 pKa = 10.86 YY58 pKa = 10.99 LNFKK62 pKa = 10.45 PEE64 pKa = 3.65 IADD67 pKa = 4.31 AIEE70 pKa = 4.79 DD71 pKa = 3.66 LAEE74 pKa = 4.7 AYY76 pKa = 9.39 TEE78 pKa = 4.09 EE79 pKa = 4.32 TGVEE83 pKa = 4.18 VKK85 pKa = 9.93 MVTAAGGTYY94 pKa = 9.41 EE95 pKa = 4.04 QTLKK99 pKa = 11.16 SEE101 pKa = 4.44 ITKK104 pKa = 10.16 SAPPTIFFINGPIGYY119 pKa = 8.76 QNWQDD124 pKa = 3.69 YY125 pKa = 8.81 TLDD128 pKa = 3.84 LADD131 pKa = 3.59 TDD133 pKa = 4.14 VYY135 pKa = 11.63 DD136 pKa = 3.64 WLVDD140 pKa = 3.34 KK141 pKa = 11.15 DD142 pKa = 4.03 LAITGADD149 pKa = 3.72 DD150 pKa = 3.58 SVAAIPITVEE160 pKa = 4.15 GYY162 pKa = 10.73 GIIYY166 pKa = 9.74 NAAIMEE172 pKa = 4.89 DD173 pKa = 4.64 YY174 pKa = 10.51 FALDD178 pKa = 3.24 NKK180 pKa = 9.98 ATDD183 pKa = 3.94 FDD185 pKa = 4.62 SVDD188 pKa = 4.56 DD189 pKa = 4.38 INNFDD194 pKa = 3.71 ALKK197 pKa = 10.58 EE198 pKa = 4.18 VVEE201 pKa = 5.4 DD202 pKa = 3.6 MTALKK207 pKa = 10.37 DD208 pKa = 3.54 DD209 pKa = 4.81 LGIQGVFSSTSLASTDD225 pKa = 2.82 QWRR228 pKa = 11.84 WQTHH232 pKa = 5.39 LANLPVYY239 pKa = 10.31 YY240 pKa = 9.8 EE241 pKa = 4.0 YY242 pKa = 11.08 RR243 pKa = 11.84 DD244 pKa = 3.64 ADD246 pKa = 3.69 VDD248 pKa = 3.98 TMSEE252 pKa = 4.04 LTFKK256 pKa = 10.91 YY257 pKa = 9.81 GKK259 pKa = 9.88 QYY261 pKa = 11.43 QNIFDD266 pKa = 5.09 LYY268 pKa = 10.72 INNSTNAPGLLGAKK282 pKa = 6.63 TTTDD286 pKa = 2.8 SMTEE290 pKa = 3.78 FALGQSAMVQNGNWGWGQISGTEE313 pKa = 3.92 GNVVNEE319 pKa = 4.22 EE320 pKa = 4.47 DD321 pKa = 3.84 VHH323 pKa = 7.04 FMPIYY328 pKa = 9.48 TGVEE332 pKa = 3.93 GEE334 pKa = 4.34 EE335 pKa = 4.03 NQGLAIGTEE344 pKa = 3.63 NFMAVNSQASEE355 pKa = 3.93 EE356 pKa = 4.71 DD357 pKa = 3.22 IQASLDD363 pKa = 3.91 FIEE366 pKa = 4.48 WMYY369 pKa = 11.44 SSEE372 pKa = 4.1 TGKK375 pKa = 10.63 DD376 pKa = 3.36 YY377 pKa = 11.42 VVNQFGFIPTFNTFGEE393 pKa = 4.73 DD394 pKa = 3.61 EE395 pKa = 4.84 FPTDD399 pKa = 4.11 PLAQDD404 pKa = 3.9 VLGSMNDD411 pKa = 3.13 EE412 pKa = 4.51 SKK414 pKa = 11.47 YY415 pKa = 9.48 SVPWTFTTFPSEE427 pKa = 3.83 AFKK430 pKa = 11.41 NDD432 pKa = 3.33 FGNGLLMYY440 pKa = 10.4 AQDD443 pKa = 3.26 QATWDD448 pKa = 3.73 EE449 pKa = 4.52 VEE451 pKa = 4.42 TTVVDD456 pKa = 3.99 AWARR460 pKa = 11.84 EE461 pKa = 3.98 AALTQQQ467 pKa = 3.58
Molecular weight: 51.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.465
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.503
Grimsley 3.363
Solomon 3.656
Lehninger 3.605
Nozaki 3.757
DTASelect 3.973
Thurlkill 3.503
EMBOSS 3.579
Sillero 3.795
Patrickios 1.316
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.711
Protein with the highest isoelectric point:
>tr|A0A6G7WH95|A0A6G7WH95_9LACT Flagellar hook-associated protein 2 OS=Jeotgalibaca porci OX=1868793 GN=fliD PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.32 KK9 pKa = 9.49 RR10 pKa = 11.84 KK11 pKa = 8.83 RR12 pKa = 11.84 QTVHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.06 RR21 pKa = 11.84 MSTKK25 pKa = 10.02 NGRR28 pKa = 11.84 NVLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2201
0
2201
663762
27
1756
301.6
33.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.427 ± 0.05
0.531 ± 0.013
5.459 ± 0.039
7.759 ± 0.062
4.446 ± 0.041
6.658 ± 0.046
1.927 ± 0.025
7.62 ± 0.052
6.252 ± 0.045
9.59 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.891 ± 0.025
4.642 ± 0.035
3.476 ± 0.025
3.68 ± 0.034
4.186 ± 0.036
5.794 ± 0.033
5.981 ± 0.038
7.042 ± 0.042
0.921 ± 0.02
3.717 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here