Periplaneta fuliginosa densovirus (isolate Guo/2000) (PfDNV)
Average proteome isoelectric point is 5.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9QCZ5|Q9QCZ5_PFDNG Structural protein OS=Periplaneta fuliginosa densovirus (isolate Guo/2000) OX=648333 PE=4 SV=1
MM1 pKa = 7.33 GSEE4 pKa = 4.12 LRR6 pKa = 11.84 PLISEE11 pKa = 4.26 VQQEE15 pKa = 4.36 RR16 pKa = 11.84 FSTDD20 pKa = 2.34 TNRR23 pKa = 11.84 IYY25 pKa = 10.87 NAGYY29 pKa = 10.11 SEE31 pKa = 3.68 IRR33 pKa = 11.84 EE34 pKa = 4.4 PNWGLRR40 pKa = 11.84 NRR42 pKa = 11.84 ISGWSEE48 pKa = 3.38 RR49 pKa = 11.84 VRR51 pKa = 11.84 EE52 pKa = 3.99 LTRR55 pKa = 11.84 PNNYY59 pKa = 9.96 SVIEE63 pKa = 4.31 GSDD66 pKa = 3.08 IEE68 pKa = 4.97 LPISVDD74 pKa = 3.72 DD75 pKa = 4.74 DD76 pKa = 3.65 VHH78 pKa = 8.34 IPIEE82 pKa = 4.36 EE83 pKa = 4.05 EE84 pKa = 3.95 TSFSVSVPEE93 pKa = 4.02 ATEE96 pKa = 4.13 TTGLLGTSVISAGTGVAGGAATGLAASDD124 pKa = 3.34 IGLGAGAVIGSGIVGYY140 pKa = 9.86 LGHH143 pKa = 7.1 KK144 pKa = 9.44 IISGLTYY151 pKa = 10.18 PFHH154 pKa = 7.51 HH155 pKa = 6.69 YY156 pKa = 10.32 LGPGNPLDD164 pKa = 3.91 NNEE167 pKa = 4.12 PVDD170 pKa = 4.24 RR171 pKa = 11.84 DD172 pKa = 3.51 DD173 pKa = 6.49 AIAEE177 pKa = 4.24 EE178 pKa = 4.41 HH179 pKa = 6.91 DD180 pKa = 3.53 KK181 pKa = 11.31 AYY183 pKa = 10.83 ANAKK187 pKa = 10.21 SSIDD191 pKa = 4.1 VINADD196 pKa = 4.27 KK197 pKa = 10.93 KK198 pKa = 11.08 AIDD201 pKa = 3.85 HH202 pKa = 6.39 FSEE205 pKa = 5.07 DD206 pKa = 3.6 FEE208 pKa = 5.85 KK209 pKa = 11.15 NGNLHH214 pKa = 6.05 SLIGKK219 pKa = 8.14 TGLQIKK225 pKa = 7.1 TAIEE229 pKa = 3.63 QRR231 pKa = 11.84 FGVIYY236 pKa = 9.67 PSVSGTT242 pKa = 3.25
Molecular weight: 25.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.713
IPC2_protein 4.711
IPC_protein 4.635
Toseland 4.52
ProMoST 4.749
Dawson 4.596
Bjellqvist 4.736
Wikipedia 4.457
Rodwell 4.495
Grimsley 4.431
Solomon 4.596
Lehninger 4.546
Nozaki 4.711
DTASelect 4.851
Thurlkill 4.507
EMBOSS 4.482
Sillero 4.774
Patrickios 3.897
IPC_peptide 4.596
IPC2_peptide 4.762
IPC2.peptide.svr19 4.71
Protein with the highest isoelectric point:
>tr|Q77NU9|Q77NU9_PFDNG Uncharacterized protein OS=Periplaneta fuliginosa densovirus (isolate Guo/2000) OX=648333 PE=4 SV=1
MM1 pKa = 7.54 QDD3 pKa = 3.55 GKK5 pKa = 10.85 QPDD8 pKa = 3.41 NSNYY12 pKa = 9.52 VPALDD17 pKa = 3.99 DD18 pKa = 3.31 TMGTVWQRR26 pKa = 11.84 MRR28 pKa = 11.84 DD29 pKa = 3.51 DD30 pKa = 5.09 AGFLDD35 pKa = 3.92 EE36 pKa = 4.31 TARR39 pKa = 11.84 DD40 pKa = 3.68 GFGGFFSRR48 pKa = 11.84 GVEE51 pKa = 3.9 NSVSSDD57 pKa = 3.59 GEE59 pKa = 3.82 ILGPQQQLQRR69 pKa = 11.84 DD70 pKa = 3.53 IVSRR74 pKa = 11.84 IKK76 pKa = 9.89 RR77 pKa = 11.84 TPFGPFRR84 pKa = 11.84 RR85 pKa = 11.84 YY86 pKa = 10.32 LSDD89 pKa = 4.25 VLACDD94 pKa = 4.73 DD95 pKa = 3.67 SRR97 pKa = 11.84 EE98 pKa = 4.19 ANRR101 pKa = 11.84 IARR104 pKa = 11.84 QVIRR108 pKa = 11.84 TARR111 pKa = 11.84 DD112 pKa = 3.41 YY113 pKa = 11.22 PGNLCLISTHH123 pKa = 7.15 DD124 pKa = 3.66 DD125 pKa = 3.48 HH126 pKa = 8.86 VHH128 pKa = 5.24 VVHH131 pKa = 7.37 DD132 pKa = 4.11 CAYY135 pKa = 10.46 SDD137 pKa = 4.05 GSCRR141 pKa = 11.84 CKK143 pKa = 10.0 ILKK146 pKa = 10.32 EE147 pKa = 3.97 EE148 pKa = 4.06 TVKK151 pKa = 10.91 AKK153 pKa = 10.44 RR154 pKa = 11.84 RR155 pKa = 11.84 GAIRR159 pKa = 11.84 KK160 pKa = 8.64 RR161 pKa = 11.84 KK162 pKa = 9.17 AISSIASDD170 pKa = 3.1 QWDD173 pKa = 4.35 DD174 pKa = 3.66 IILYY178 pKa = 9.02 FISNGRR184 pKa = 11.84 WLQFAKK190 pKa = 10.77 VGGSVVGLPYY200 pKa = 10.41 GYY202 pKa = 9.95 QALQEE207 pKa = 4.55 RR208 pKa = 11.84 GHH210 pKa = 6.05 QGQDD214 pKa = 2.45 SGQILGTCTSTGSSEE229 pKa = 4.27 LCGEE233 pKa = 4.2 SSIEE237 pKa = 4.03 EE238 pKa = 4.17 VTGKK242 pKa = 9.27 SARR245 pKa = 11.84 INSRR249 pKa = 11.84 DD250 pKa = 2.75 GRR252 pKa = 11.84 RR253 pKa = 11.84 RR254 pKa = 11.84 RR255 pKa = 11.84 RR256 pKa = 11.84 RR257 pKa = 11.84 ASDD260 pKa = 2.69 IRR262 pKa = 11.84 EE263 pKa = 3.93 EE264 pKa = 3.97 MEE266 pKa = 4.8 KK267 pKa = 10.06 IVNEE271 pKa = 4.07 NPVCPLAGIISTRR284 pKa = 11.84 QWLTHH289 pKa = 6.02 KK290 pKa = 10.49 DD291 pKa = 3.53 LRR293 pKa = 11.84 YY294 pKa = 10.07 LRR296 pKa = 11.84 ADD298 pKa = 3.39 NEE300 pKa = 4.81 TVKK303 pKa = 10.98 SFLDD307 pKa = 3.55 AKK309 pKa = 10.51 SEE311 pKa = 4.0 EE312 pKa = 3.92 MCYY315 pKa = 7.77 WTLHH319 pKa = 6.43 DD320 pKa = 5.31 FNTFYY325 pKa = 10.95 SQPYY329 pKa = 9.1 CNPTFMAGYY338 pKa = 9.8 QPLEE342 pKa = 4.06 DD343 pKa = 4.26 KK344 pKa = 10.85 YY345 pKa = 11.68 YY346 pKa = 10.98 SVNDD350 pKa = 3.56 SLTILNKK357 pKa = 10.31 LLAYY361 pKa = 10.42 QFDD364 pKa = 4.54 DD365 pKa = 4.23 DD366 pKa = 4.33 VVQIKK371 pKa = 10.75 SFLNTFYY378 pKa = 11.37 NVLEE382 pKa = 4.39 RR383 pKa = 11.84 KK384 pKa = 9.35 LPKK387 pKa = 10.55 CNTICVWSPPSAGKK401 pKa = 10.0 NFFFDD406 pKa = 3.69 VYY408 pKa = 10.89 LHH410 pKa = 6.09 YY411 pKa = 11.21 LMNYY415 pKa = 8.03 GQLGIMNKK423 pKa = 8.69 TNNFSLQEE431 pKa = 4.05 ATSKK435 pKa = 10.72 RR436 pKa = 11.84 VLLWNEE442 pKa = 3.63 PNYY445 pKa = 10.81 EE446 pKa = 3.97 DD447 pKa = 6.12 AYY449 pKa = 10.07 TDD451 pKa = 3.63 TLKK454 pKa = 10.55 MLTGGDD460 pKa = 3.89 ALCVRR465 pKa = 11.84 VKK467 pKa = 10.36 QKK469 pKa = 10.85 KK470 pKa = 9.01 DD471 pKa = 2.96 CHH473 pKa = 6.43 VYY475 pKa = 8.51 KK476 pKa = 10.51 TPLIVLTNNMIGFMHH491 pKa = 7.01 EE492 pKa = 3.82 LAFVDD497 pKa = 3.87 RR498 pKa = 11.84 VKK500 pKa = 10.84 VYY502 pKa = 9.69 RR503 pKa = 11.84 WKK505 pKa = 10.37 QAPFLAEE512 pKa = 4.06 YY513 pKa = 10.07 NKK515 pKa = 10.57 KK516 pKa = 10.12 PNPLVAFEE524 pKa = 4.39 ILVYY528 pKa = 10.11 WEE530 pKa = 4.46 IIPRR534 pKa = 11.84 IIEE537 pKa = 3.79 QQ538 pKa = 3.25
Molecular weight: 61.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.845
IPC2_protein 7.146
IPC_protein 7.029
Toseland 6.737
ProMoST 7.556
Dawson 7.702
Bjellqvist 8.083
Wikipedia 7.614
Rodwell 7.717
Grimsley 6.795
Solomon 7.746
Lehninger 7.761
Nozaki 8.287
DTASelect 7.863
Thurlkill 7.907
EMBOSS 7.936
Sillero 8.229
Patrickios 4.151
IPC_peptide 7.732
IPC2_peptide 7.293
IPC2.peptide.svr19 7.232
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2180
121
574
311.4
35.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.413 ± 0.567
1.972 ± 0.545
5.963 ± 0.573
6.239 ± 0.643
3.028 ± 0.336
5.78 ± 1.104
2.294 ± 0.301
6.376 ± 0.671
5.367 ± 0.426
8.349 ± 0.784
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.345
5.459 ± 0.548
4.862 ± 0.732
4.679 ± 0.651
4.862 ± 0.719
7.523 ± 0.73
6.697 ± 0.583
6.697 ± 0.596
1.56 ± 0.448
4.633 ± 0.462
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here