Amino acid dipepetide frequency for Microbacterium sp. AR7-10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.351AlaAla: 20.351 ± 0.23	0.673AlaCys: 0.673 ± 0.032	8.903AlaAsp: 8.903 ± 0.126	8.558AlaGlu: 8.558 ± 0.118	3.928AlaPhe: 3.928 ± 0.07	11.815AlaGly: 11.815 ± 0.135	2.577AlaHis: 2.577 ± 0.062	5.884AlaIle: 5.884 ± 0.096	2.812AlaLys: 2.812 ± 0.062	14.257AlaLeu: 14.257 ± 0.142	2.803AlaMet: 2.803 ± 0.06	2.156AlaAsn: 2.156 ± 0.057	6.473AlaPro: 6.473 ± 0.122	4.1AlaGln: 4.1 ± 0.084	9.168AlaArg: 9.168 ± 0.131	7.22AlaSer: 7.22 ± 0.075	6.996AlaThr: 6.996 ± 0.088	11.733AlaVal: 11.733 ± 0.141	1.823AlaTrp: 1.823 ± 0.048	2.303AlaTyr: 2.303 ± 0.052	0.002AlaXaa: 0.002 ± 0.002
Cys	0.662CysAla: 0.662 ± 0.028	0.041CysCys: 0.041 ± 0.007	0.326CysAsp: 0.326 ± 0.018	0.275CysGlu: 0.275 ± 0.02	0.168CysPhe: 0.168 ± 0.014	0.552CysGly: 0.552 ± 0.029	0.111CysHis: 0.111 ± 0.012	0.221CysIle: 0.221 ± 0.016	0.058CysLys: 0.058 ± 0.008	0.414CysLeu: 0.414 ± 0.02	0.067CysMet: 0.067 ± 0.009	0.112CysAsn: 0.112 ± 0.011	0.285CysPro: 0.285 ± 0.02	0.119CysGln: 0.119 ± 0.011	0.342CysArg: 0.342 ± 0.023	0.303CysSer: 0.303 ± 0.02	0.326CysThr: 0.326 ± 0.02	0.387CysVal: 0.387 ± 0.018	0.071CysTrp: 0.071 ± 0.009	0.094CysTyr: 0.094 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	10.213AspAla: 10.213 ± 0.112	0.217AspCys: 0.217 ± 0.016	4.82AspAsp: 4.82 ± 0.085	4.816AspGlu: 4.816 ± 0.076	1.763AspPhe: 1.763 ± 0.055	6.318AspGly: 6.318 ± 0.111	1.145AspHis: 1.145 ± 0.037	2.555AspIle: 2.555 ± 0.066	1.022AspLys: 1.022 ± 0.037	6.13AspLeu: 6.13 ± 0.085	0.942AspMet: 0.942 ± 0.03	0.89AspAsn: 0.89 ± 0.036	4.071AspPro: 4.071 ± 0.079	1.597AspGln: 1.597 ± 0.046	4.524AspArg: 4.524 ± 0.093	2.737AspSer: 2.737 ± 0.049	2.807AspThr: 2.807 ± 0.06	5.664AspVal: 5.664 ± 0.097	0.927AspTrp: 0.927 ± 0.03	1.262AspTyr: 1.262 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	6.855GluAla: 6.855 ± 0.103	0.242GluCys: 0.242 ± 0.018	2.629GluAsp: 2.629 ± 0.052	2.846GluGlu: 2.846 ± 0.075	1.754GluPhe: 1.754 ± 0.044	4.114GluGly: 4.114 ± 0.074	1.587GluHis: 1.587 ± 0.041	2.929GluIle: 2.929 ± 0.058	1.618GluLys: 1.618 ± 0.048	6.327GluLeu: 6.327 ± 0.089	1.086GluMet: 1.086 ± 0.034	1.319GluAsn: 1.319 ± 0.042	2.859GluPro: 2.859 ± 0.063	2.482GluGln: 2.482 ± 0.055	5.53GluArg: 5.53 ± 0.093	2.908GluSer: 2.908 ± 0.05	3.062GluThr: 3.062 ± 0.054	4.638GluVal: 4.638 ± 0.074	0.904GluTrp: 0.904 ± 0.036	1.254GluTyr: 1.254 ± 0.041	0.001GluXaa: 0.001 ± 0.001
Phe	4.322PheAla: 4.322 ± 0.084	0.175PheCys: 0.175 ± 0.014	2.47PheAsp: 2.47 ± 0.054	1.719PheGlu: 1.719 ± 0.046	1.116PhePhe: 1.116 ± 0.038	3.469PheGly: 3.469 ± 0.066	0.544PheHis: 0.544 ± 0.025	1.262PheIle: 1.262 ± 0.038	0.483PheLys: 0.483 ± 0.023	2.748PheLeu: 2.748 ± 0.061	0.475PheMet: 0.475 ± 0.024	0.656PheAsn: 0.656 ± 0.028	1.409PhePro: 1.409 ± 0.04	0.78PheGln: 0.78 ± 0.031	1.767PheArg: 1.767 ± 0.044	1.715PheSer: 1.715 ± 0.046	2.129PheThr: 2.129 ± 0.053	2.744PheVal: 2.744 ± 0.058	0.477PheTrp: 0.477 ± 0.028	0.607PheTyr: 0.607 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.862GlyAla: 10.862 ± 0.151	0.53GlyCys: 0.53 ± 0.026	5.218GlyAsp: 5.218 ± 0.089	5.208GlyGlu: 5.208 ± 0.07	3.221GlyPhe: 3.221 ± 0.061	7.522GlyGly: 7.522 ± 0.113	1.808GlyHis: 1.808 ± 0.052	5.079GlyIle: 5.079 ± 0.082	2.063GlyLys: 2.063 ± 0.054	8.438GlyLeu: 8.438 ± 0.115	2.144GlyMet: 2.144 ± 0.048	1.543GlyAsn: 1.543 ± 0.05	3.477GlyPro: 3.477 ± 0.064	2.495GlyGln: 2.495 ± 0.055	6.532GlyArg: 6.532 ± 0.092	5.189GlySer: 5.189 ± 0.081	5.247GlyThr: 5.247 ± 0.082	7.899GlyVal: 7.899 ± 0.095	1.654GlyTrp: 1.654 ± 0.053	2.195GlyTyr: 2.195 ± 0.054	0.003GlyXaa: 0.003 ± 0.002
His	2.484HisAla: 2.484 ± 0.057	0.109HisCys: 0.109 ± 0.011	1.442HisAsp: 1.442 ± 0.04	1.223HisGlu: 1.223 ± 0.039	0.53HisPhe: 0.53 ± 0.026	1.991HisGly: 1.991 ± 0.052	0.561HisHis: 0.561 ± 0.025	0.783HisIle: 0.783 ± 0.032	0.308HisLys: 0.308 ± 0.02	2.055HisLeu: 2.055 ± 0.057	0.342HisMet: 0.342 ± 0.02	0.329HisAsn: 0.329 ± 0.019	1.564HisPro: 1.564 ± 0.042	0.476HisGln: 0.476 ± 0.022	1.763HisArg: 1.763 ± 0.05	0.994HisSer: 0.994 ± 0.03	0.97HisThr: 0.97 ± 0.032	1.69HisVal: 1.69 ± 0.038	0.299HisTrp: 0.299 ± 0.02	0.4HisTyr: 0.4 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.374IleAla: 7.374 ± 0.094	0.257IleCys: 0.257 ± 0.017	3.829IleAsp: 3.829 ± 0.072	3.067IleGlu: 3.067 ± 0.061	1.191IlePhe: 1.191 ± 0.04	4.977IleGly: 4.977 ± 0.087	0.691IleHis: 0.691 ± 0.025	2.109IleIle: 2.109 ± 0.057	0.787IleLys: 0.787 ± 0.034	3.722IleLeu: 3.722 ± 0.068	0.699IleMet: 0.699 ± 0.026	0.933IleAsn: 0.933 ± 0.036	2.519IlePro: 2.519 ± 0.053	1.04IleGln: 1.04 ± 0.036	2.993IleArg: 2.993 ± 0.059	2.515IleSer: 2.515 ± 0.056	2.943IleThr: 2.943 ± 0.069	4.855IleVal: 4.855 ± 0.086	0.591IleTrp: 0.591 ± 0.027	0.753IleTyr: 0.753 ± 0.029	0.001IleXaa: 0.001 ± 0.001
Lys	2.525LysAla: 2.525 ± 0.067	0.069LysCys: 0.069 ± 0.009	1.228LysAsp: 1.228 ± 0.042	0.979LysGlu: 0.979 ± 0.037	0.466LysPhe: 0.466 ± 0.022	1.626LysGly: 1.626 ± 0.044	0.476LysHis: 0.476 ± 0.027	0.951LysIle: 0.951 ± 0.041	0.784LysLys: 0.784 ± 0.04	1.906LysLeu: 1.906 ± 0.049	0.42LysMet: 0.42 ± 0.024	0.559LysAsn: 0.559 ± 0.028	1.145LysPro: 1.145 ± 0.039	0.755LysGln: 0.755 ± 0.029	1.459LysArg: 1.459 ± 0.047	1.164LysSer: 1.164 ± 0.04	1.368LysThr: 1.368 ± 0.043	1.689LysVal: 1.689 ± 0.049	0.251LysTrp: 0.251 ± 0.018	0.425LysTyr: 0.425 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.944LeuAla: 13.944 ± 0.142	0.523LeuCys: 0.523 ± 0.026	6.589LeuAsp: 6.589 ± 0.092	5.07LeuGlu: 5.07 ± 0.09	3.008LeuPhe: 3.008 ± 0.063	8.934LeuGly: 8.934 ± 0.118	1.95LeuHis: 1.95 ± 0.048	4.948LeuIle: 4.948 ± 0.089	1.726LeuLys: 1.726 ± 0.047	10.105LeuLeu: 10.105 ± 0.139	1.833LeuMet: 1.833 ± 0.048	1.82LeuAsn: 1.82 ± 0.043	5.372LeuPro: 5.372 ± 0.078	2.665LeuGln: 2.665 ± 0.055	7.74LeuArg: 7.74 ± 0.114	5.921LeuSer: 5.921 ± 0.094	6.258LeuThr: 6.258 ± 0.096	8.734LeuVal: 8.734 ± 0.1	1.317LeuTrp: 1.317 ± 0.046	1.673LeuTyr: 1.673 ± 0.044	0.001LeuXaa: 0.001 ± 0.001
Met	2.275MetAla: 2.275 ± 0.051	0.126MetCys: 0.126 ± 0.012	0.874MetAsp: 0.874 ± 0.035	0.622MetGlu: 0.622 ± 0.027	0.564MetPhe: 0.564 ± 0.024	1.462MetGly: 1.462 ± 0.043	0.377MetHis: 0.377 ± 0.021	0.984MetIle: 0.984 ± 0.031	0.464MetLys: 0.464 ± 0.027	2.353MetLeu: 2.353 ± 0.055	0.416MetMet: 0.416 ± 0.024	0.501MetAsn: 0.501 ± 0.021	1.272MetPro: 1.272 ± 0.034	0.643MetGln: 0.643 ± 0.028	1.626MetArg: 1.626 ± 0.044	1.542MetSer: 1.542 ± 0.041	1.711MetThr: 1.711 ± 0.047	1.422MetVal: 1.422 ± 0.04	0.254MetTrp: 0.254 ± 0.018	0.324MetTyr: 0.324 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.311AsnAla: 2.311 ± 0.057	0.1AsnCys: 0.1 ± 0.01	1.146AsnAsp: 1.146 ± 0.041	0.978AsnGlu: 0.978 ± 0.032	0.589AsnPhe: 0.589 ± 0.024	1.868AsnGly: 1.868 ± 0.054	0.34AsnHis: 0.34 ± 0.023	0.861AsnIle: 0.861 ± 0.033	0.403AsnLys: 0.403 ± 0.022	1.83AsnLeu: 1.83 ± 0.043	0.353AsnMet: 0.353 ± 0.019	0.435AsnAsn: 0.435 ± 0.024	1.508AsnPro: 1.508 ± 0.039	0.55AsnGln: 0.55 ± 0.03	1.275AsnArg: 1.275 ± 0.033	0.95AsnSer: 0.95 ± 0.036	1.074AsnThr: 1.074 ± 0.035	1.567AsnVal: 1.567 ± 0.046	0.296AsnTrp: 0.296 ± 0.019	0.408AsnTyr: 0.408 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	7.108ProAla: 7.108 ± 0.104	0.191ProCys: 0.191 ± 0.016	4.021ProAsp: 4.021 ± 0.069	3.675ProGlu: 3.675 ± 0.057	1.746ProPhe: 1.746 ± 0.042	4.605ProGly: 4.605 ± 0.078	1.142ProHis: 1.142 ± 0.038	2.15ProIle: 2.15 ± 0.05	1.015ProLys: 1.015 ± 0.037	4.926ProLeu: 4.926 ± 0.075	0.972ProMet: 0.972 ± 0.03	1.01ProAsn: 1.01 ± 0.033	2.201ProPro: 2.201 ± 0.065	1.666ProGln: 1.666 ± 0.04	3.307ProArg: 3.307 ± 0.063	2.926ProSer: 2.926 ± 0.064	3.166ProThr: 3.166 ± 0.058	4.793ProVal: 4.793 ± 0.082	0.829ProTrp: 0.829 ± 0.032	1.031ProTyr: 1.031 ± 0.035	0.002ProXaa: 0.002 ± 0.002
Gln	3.62GlnAla: 3.62 ± 0.069	0.108GlnCys: 0.108 ± 0.011	1.358GlnAsp: 1.358 ± 0.039	1.315GlnGlu: 1.315 ± 0.039	0.764GlnPhe: 0.764 ± 0.033	2.189GlnGly: 2.189 ± 0.051	0.734GlnHis: 0.734 ± 0.033	1.56GlnIle: 1.56 ± 0.047	0.793GlnLys: 0.793 ± 0.03	3.347GlnLeu: 3.347 ± 0.071	0.56GlnMet: 0.56 ± 0.025	0.684GlnAsn: 0.684 ± 0.028	1.645GlnPro: 1.645 ± 0.045	1.306GlnGln: 1.306 ± 0.051	2.723GlnArg: 2.723 ± 0.061	1.58GlnSer: 1.58 ± 0.036	1.634GlnThr: 1.634 ± 0.042	2.393GlnVal: 2.393 ± 0.05	0.476GlnTrp: 0.476 ± 0.025	0.605GlnTyr: 0.605 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	9.096ArgAla: 9.096 ± 0.119	0.312ArgCys: 0.312 ± 0.019	4.67ArgAsp: 4.67 ± 0.074	4.483ArgGlu: 4.483 ± 0.069	2.502ArgPhe: 2.502 ± 0.053	5.595ArgGly: 5.595 ± 0.087	1.595ArgHis: 1.595 ± 0.044	3.836ArgIle: 3.836 ± 0.059	1.406ArgLys: 1.406 ± 0.047	7.375ArgLeu: 7.375 ± 0.117	2.014ArgMet: 2.014 ± 0.045	1.283ArgAsn: 1.283 ± 0.041	3.679ArgPro: 3.679 ± 0.067	2.153ArgGln: 2.153 ± 0.053	6.846ArgArg: 6.846 ± 0.119	4.234ArgSer: 4.234 ± 0.08	4.488ArgThr: 4.488 ± 0.069	6.245ArgVal: 6.245 ± 0.086	1.23ArgTrp: 1.23 ± 0.045	1.536ArgTyr: 1.536 ± 0.037	0.003ArgXaa: 0.003 ± 0.002
Ser	7.233SerAla: 7.233 ± 0.088	0.277SerCys: 0.277 ± 0.018	3.326SerAsp: 3.326 ± 0.066	2.673SerGlu: 2.673 ± 0.062	1.861SerPhe: 1.861 ± 0.041	5.675SerGly: 5.675 ± 0.079	1.023SerHis: 1.023 ± 0.04	2.748SerIle: 2.748 ± 0.055	1.123SerLys: 1.123 ± 0.041	5.308SerLeu: 5.308 ± 0.078	1.291SerMet: 1.291 ± 0.043	1.046SerAsn: 1.046 ± 0.034	2.932SerPro: 2.932 ± 0.056	1.464SerGln: 1.464 ± 0.044	3.858SerArg: 3.858 ± 0.074	3.35SerSer: 3.35 ± 0.068	3.51SerThr: 3.51 ± 0.068	4.528SerVal: 4.528 ± 0.065	0.96SerTrp: 0.96 ± 0.036	1.15SerTyr: 1.15 ± 0.036	0.002SerXaa: 0.002 ± 0.002
Thr	7.934ThrAla: 7.934 ± 0.113	0.285ThrCys: 0.285 ± 0.018	3.56ThrAsp: 3.56 ± 0.068	2.933ThrGlu: 2.933 ± 0.061	1.822ThrPhe: 1.822 ± 0.045	5.667ThrGly: 5.667 ± 0.082	1.164ThrHis: 1.164 ± 0.033	2.833ThrIle: 2.833 ± 0.058	1.107ThrLys: 1.107 ± 0.041	5.847ThrLeu: 5.847 ± 0.09	1.057ThrMet: 1.057 ± 0.038	1.06ThrAsn: 1.06 ± 0.037	3.725ThrPro: 3.725 ± 0.069	1.485ThrGln: 1.485 ± 0.038	3.9ThrArg: 3.9 ± 0.077	3.243ThrSer: 3.243 ± 0.061	3.589ThrThr: 3.589 ± 0.073	5.612ThrVal: 5.612 ± 0.089	0.825ThrTrp: 0.825 ± 0.032	1.088ThrTyr: 1.088 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	11.285ValAla: 11.285 ± 0.126	0.495ValCys: 0.495 ± 0.023	5.828ValAsp: 5.828 ± 0.085	4.81ValGlu: 4.81 ± 0.086	2.805ValPhe: 2.805 ± 0.063	6.948ValGly: 6.948 ± 0.098	1.836ValHis: 1.836 ± 0.046	4.695ValIle: 4.695 ± 0.08	1.642ValLys: 1.642 ± 0.047	9.372ValLeu: 9.372 ± 0.122	1.633ValMet: 1.633 ± 0.048	1.663ValAsn: 1.663 ± 0.044	4.626ValPro: 4.626 ± 0.083	2.432ValGln: 2.432 ± 0.049	6.285ValArg: 6.285 ± 0.094	4.844ValSer: 4.844 ± 0.079	5.44ValThr: 5.44 ± 0.092	8.424ValVal: 8.424 ± 0.121	1.099ValTrp: 1.099 ± 0.033	1.551ValTyr: 1.551 ± 0.045	0.001ValXaa: 0.001 ± 0.001
Trp	1.692TrpAla: 1.692 ± 0.044	0.087TrpCys: 0.087 ± 0.008	0.814TrpAsp: 0.814 ± 0.032	0.602TrpGlu: 0.602 ± 0.03	0.563TrpPhe: 0.563 ± 0.029	1.121TrpGly: 1.121 ± 0.036	0.384TrpHis: 0.384 ± 0.021	0.686TrpIle: 0.686 ± 0.027	0.289TrpLys: 0.289 ± 0.019	1.692TrpLeu: 1.692 ± 0.053	0.408TrpMet: 0.408 ± 0.023	0.429TrpAsn: 0.429 ± 0.022	0.737TrpPro: 0.737 ± 0.033	0.593TrpGln: 0.593 ± 0.024	1.285TrpArg: 1.285 ± 0.04	0.874TrpSer: 0.874 ± 0.033	0.907TrpThr: 0.907 ± 0.031	1.158TrpVal: 1.158 ± 0.038	0.372TrpTrp: 0.372 ± 0.021	0.263TrpTyr: 0.263 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.387TyrAla: 2.387 ± 0.057	0.117TyrCys: 0.117 ± 0.012	1.367TyrAsp: 1.367 ± 0.04	1.095TyrGlu: 1.095 ± 0.038	0.706TyrPhe: 0.706 ± 0.029	1.877TyrGly: 1.877 ± 0.045	0.27TyrHis: 0.27 ± 0.016	0.771TyrIle: 0.771 ± 0.029	0.344TyrLys: 0.344 ± 0.019	1.975TyrLeu: 1.975 ± 0.046	0.296TyrMet: 0.296 ± 0.02	0.415TyrAsn: 0.415 ± 0.024	0.978TyrPro: 0.978 ± 0.032	0.593TyrGln: 0.593 ± 0.026	1.656TyrArg: 1.656 ± 0.044	1.13TyrSer: 1.13 ± 0.036	1.111TyrThr: 1.111 ± 0.04	1.538TyrVal: 1.538 ± 0.036	0.299TyrTrp: 0.299 ± 0.019	0.438TyrTyr: 0.438 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.002	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2773 proteins (892355 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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