Edaphobacter dinghuensis

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Edaphobacter

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3723 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495BWF8|A0A495BWF8_9BACT Elongation factor G OS=Edaphobacter dinghuensis OX=1560005 GN=fusA PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 10.29ILLAVLALTTTVAMAPAAKK21 pKa = 9.84ADD23 pKa = 3.6SFNFSFSGGGLSATGVIDD41 pKa = 3.55VSSAVPGVAGAYY53 pKa = 9.08QVTGIHH59 pKa = 6.47GVFSDD64 pKa = 4.48ANTGLTNASITGLVNTSLPSPINGDD89 pKa = 3.71GTFLPPGTFALTQPAGDD106 pKa = 4.14EE107 pKa = 4.18GLSFDD112 pKa = 4.84NLFYY116 pKa = 10.8PNGDD120 pKa = 3.55SPAICPPSITEE131 pKa = 3.67PGYY134 pKa = 9.37PFHH137 pKa = 7.1GGSLDD142 pKa = 3.07IYY144 pKa = 10.4GVLFTVDD151 pKa = 2.68GGYY154 pKa = 8.06TVDD157 pKa = 2.98MWSNGVLPGTTAPDD171 pKa = 3.6YY172 pKa = 11.2GVTDD176 pKa = 5.17AIGTTRR182 pKa = 11.84GNSADD187 pKa = 3.44VSFAASPVPEE197 pKa = 5.46PGTLMLLGTGMLGFAGSMVRR217 pKa = 11.84KK218 pKa = 9.63FKK220 pKa = 11.22QNAA223 pKa = 3.08

Molecular weight: 22.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495BUP5|A0A495BUP5_9BACT dUTP diphosphatase OS=Edaphobacter dinghuensis OX=1560005 GN=C8E94_2095 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.69RR13 pKa = 11.84AKK15 pKa = 9.55THH17 pKa = 5.47GFLTRR22 pKa = 11.84MKK24 pKa = 9.03TKK26 pKa = 10.57AGAAVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.72KK42 pKa = 10.29IAVSAGFRR50 pKa = 11.84DD51 pKa = 3.66

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3723

0

3723

1291713

27

2459

347.0

37.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.366 ± 0.048

0.859 ± 0.013

5.056 ± 0.028

5.382 ± 0.053

4.007 ± 0.028

7.884 ± 0.044

2.338 ± 0.019

5.447 ± 0.033

3.995 ± 0.036

9.906 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.019

3.587 ± 0.041

5.135 ± 0.031

3.853 ± 0.027

5.82 ± 0.043

6.573 ± 0.041

6.226 ± 0.045

7.143 ± 0.037

1.304 ± 0.017

2.743 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski