Macaca mulatta feces associated virus 9
Average proteome isoelectric point is 8.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5PZN0|A0A1W5PZN0_9VIRU Cap OS=Macaca mulatta feces associated virus 9 OX=2499231 PE=4 SV=1
MM1 pKa = 6.77 MSYY4 pKa = 10.33 RR5 pKa = 11.84 KK6 pKa = 9.54 RR7 pKa = 11.84 RR8 pKa = 11.84 GSKK11 pKa = 9.55 VLHH14 pKa = 6.49 QKK16 pKa = 8.52 FQWFFDD22 pKa = 3.94 LQTKK26 pKa = 9.57 ADD28 pKa = 3.7 EE29 pKa = 4.12 MQIIEE34 pKa = 4.4 VSAGGYY40 pKa = 9.27 GVYY43 pKa = 10.2 KK44 pKa = 10.55 RR45 pKa = 11.84 LFPFFSAFKK54 pKa = 8.75 YY55 pKa = 10.37 YY56 pKa = 10.95 KK57 pKa = 10.56 LGGVKK62 pKa = 9.37 MKK64 pKa = 9.33 MIPASTLPVDD74 pKa = 3.87 PTGLSYY80 pKa = 10.68 EE81 pKa = 4.23 AGEE84 pKa = 4.26 NTVDD88 pKa = 4.85 PRR90 pKa = 11.84 DD91 pKa = 3.48 QLTPGLTRR99 pKa = 11.84 ITNGEE104 pKa = 4.0 DD105 pKa = 3.58 VYY107 pKa = 11.46 TKK109 pKa = 11.0 LDD111 pKa = 3.67 GLTGNQQRR119 pKa = 11.84 EE120 pKa = 4.68 LYY122 pKa = 9.13 EE123 pKa = 4.83 SMMIDD128 pKa = 3.25 QRR130 pKa = 11.84 WFKK133 pKa = 10.13 WQLQSGLSRR142 pKa = 11.84 YY143 pKa = 8.4 ARR145 pKa = 11.84 PMYY148 pKa = 8.75 WQIGQLHH155 pKa = 6.12 QDD157 pKa = 3.76 YY158 pKa = 11.0 LPGSVRR164 pKa = 11.84 NLADD168 pKa = 3.42 TNLTEE173 pKa = 4.61 DD174 pKa = 4.17 CQSISAVYY182 pKa = 10.49 DD183 pKa = 3.36 GANPSGPSAGPITVAADD200 pKa = 3.17 ASDD203 pKa = 3.55 PRR205 pKa = 11.84 GLFQVGHH212 pKa = 7.01 RR213 pKa = 11.84 GRR215 pKa = 11.84 LGWMPTDD222 pKa = 3.53 GVLLKK227 pKa = 9.81 GTSGGDD233 pKa = 3.59 GTVHH237 pKa = 5.98 AQAVEE242 pKa = 3.92 AAIPAVNVFTIVTPPMHH259 pKa = 6.15 KK260 pKa = 8.5 TNYY263 pKa = 7.64 YY264 pKa = 8.29 YY265 pKa = 10.56 RR266 pKa = 11.84 VFVTEE271 pKa = 4.34 DD272 pKa = 3.84 VYY274 pKa = 11.28 FKK276 pKa = 11.2 SPVVVGYY283 pKa = 10.0 QNFRR287 pKa = 11.84 SIDD290 pKa = 3.53 RR291 pKa = 11.84 FVQPQFPVAKK301 pKa = 9.97 LPTVNSATTDD311 pKa = 3.39 SNKK314 pKa = 9.75 PFPTNDD320 pKa = 3.19 GGEE323 pKa = 4.25 LPGVIQQ329 pKa = 4.56
Molecular weight: 36.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.576
IPC2_protein 7.79
IPC_protein 7.966
Toseland 8.258
ProMoST 8.141
Dawson 8.565
Bjellqvist 8.419
Wikipedia 8.682
Rodwell 8.58
Grimsley 8.346
Solomon 8.799
Lehninger 8.741
Nozaki 8.375
DTASelect 8.434
Thurlkill 8.463
EMBOSS 8.726
Sillero 8.639
Patrickios 4.202
IPC_peptide 8.785
IPC2_peptide 7.351
IPC2.peptide.svr19 7.563
Protein with the highest isoelectric point:
>tr|A0A1W5PZN0|A0A1W5PZN0_9VIRU Cap OS=Macaca mulatta feces associated virus 9 OX=2499231 PE=4 SV=1
MM1 pKa = 7.2 CWLYY5 pKa = 11.25 KK6 pKa = 10.07 FAPKK10 pKa = 9.93 PIPAPARR17 pKa = 11.84 VDD19 pKa = 3.22 KK20 pKa = 11.03 ASRR23 pKa = 11.84 GSINSASRR31 pKa = 11.84 NSYY34 pKa = 9.71 HH35 pKa = 6.89 ICHH38 pKa = 6.32 TCEE41 pKa = 3.69 TFRR44 pKa = 11.84 GCGSLSCSTLFRR56 pKa = 11.84 TSLFITVQHH65 pKa = 5.32 CTIMYY70 pKa = 7.67 MAAQTNYY77 pKa = 10.39 KK78 pKa = 9.6 FRR80 pKa = 11.84 QRR82 pKa = 11.84 LQSIMTHH89 pKa = 4.25 TWMLTVPRR97 pKa = 11.84 RR98 pKa = 11.84 KK99 pKa = 7.6 TARR102 pKa = 11.84 EE103 pKa = 3.42 WMGISKK109 pKa = 9.41 WLRR112 pKa = 11.84 DD113 pKa = 3.56 NDD115 pKa = 3.66 VHH117 pKa = 6.67 KK118 pKa = 9.25 WTVAMEE124 pKa = 4.39 TGDD127 pKa = 3.25 NGYY130 pKa = 9.67 DD131 pKa = 2.78 HH132 pKa = 6.76 WQIRR136 pKa = 11.84 LQVNKK141 pKa = 9.04 TFKK144 pKa = 10.1 QLKK147 pKa = 9.6 KK148 pKa = 8.91 EE149 pKa = 4.17 WGPKK153 pKa = 9.16 AHH155 pKa = 6.94 IEE157 pKa = 4.11 EE158 pKa = 5.74 ASDD161 pKa = 2.87 TWNYY165 pKa = 8.1 EE166 pKa = 3.95 RR167 pKa = 11.84 KK168 pKa = 9.95 SGLFFSSNDD177 pKa = 3.29 TPEE180 pKa = 3.66 VRR182 pKa = 11.84 KK183 pKa = 10.19 CRR185 pKa = 11.84 FGRR188 pKa = 11.84 LTWRR192 pKa = 11.84 QQAVVQAVQGSNDD205 pKa = 3.33 RR206 pKa = 11.84 QIVVWYY212 pKa = 9.66 DD213 pKa = 3.15 PNGNAGKK220 pKa = 9.94 SWLLGHH226 pKa = 7.53 LYY228 pKa = 8.31 EE229 pKa = 5.2 TGQAWVVQAQDD240 pKa = 3.45 TVKK243 pKa = 11.18 GIIQDD248 pKa = 3.78 CASEE252 pKa = 4.39 YY253 pKa = 9.83 IQHH256 pKa = 5.81 GWRR259 pKa = 11.84 PFVVIDD265 pKa = 5.11 IPRR268 pKa = 11.84 SWKK271 pKa = 7.84 WTQEE275 pKa = 3.77 LYY277 pKa = 10.93 VALEE281 pKa = 4.21 RR282 pKa = 11.84 IKK284 pKa = 11.05 DD285 pKa = 3.81 GLIKK289 pKa = 10.46 DD290 pKa = 3.65 PRR292 pKa = 11.84 YY293 pKa = 10.2 GSKK296 pKa = 8.91 TVHH299 pKa = 5.15 IRR301 pKa = 11.84 GVKK304 pKa = 10.11 VLVTCNTKK312 pKa = 10.43 PKK314 pKa = 10.02 LDD316 pKa = 3.92 KK317 pKa = 10.98 LSADD321 pKa = 3.09 RR322 pKa = 11.84 WVIMNMNTLEE332 pKa = 4.27 AEE334 pKa = 4.33 CAAMEE339 pKa = 4.18 VADD342 pKa = 6.46 RR343 pKa = 11.84 IWEE346 pKa = 4.1 DD347 pKa = 2.99
Molecular weight: 40.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.819
IPC2_protein 8.653
IPC_protein 8.536
Toseland 9.194
ProMoST 9.077
Dawson 9.516
Bjellqvist 9.385
Wikipedia 9.663
Rodwell 9.75
Grimsley 9.531
Solomon 9.56
Lehninger 9.531
Nozaki 9.575
DTASelect 9.268
Thurlkill 9.414
EMBOSS 9.677
Sillero 9.575
Patrickios 4.889
IPC_peptide 9.56
IPC2_peptide 8.229
IPC2.peptide.svr19 7.791
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
676
329
347
338.0
38.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.361 ± 0.177
1.627 ± 0.832
5.621 ± 0.288
3.994 ± 0.409
3.846 ± 0.639
7.396 ± 1.273
2.219 ± 0.439
4.734 ± 0.683
5.917 ± 0.662
6.509 ± 0.303
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.959 ± 0.051
3.846 ± 0.125
5.178 ± 1.14
5.621 ± 0.288
6.213 ± 0.657
6.065 ± 0.009
6.953 ± 0.024
7.544 ± 0.416
3.254 ± 1.09
4.142 ± 0.644
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here