Monosporascus sp. MG133

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariales incertae sedis; Monosporascus; unclassified Monosporascus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11566 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4V6C8|A0A4Q4V6C8_9PEZI Uncharacterized protein OS=Monosporascus sp. MG133 OX=2211645 GN=DL767_006947 PE=4 SV=1
MM1 pKa = 7.99RR2 pKa = 11.84KK3 pKa = 8.75PLSVSKK9 pKa = 10.31RR10 pKa = 11.84QEE12 pKa = 3.88DD13 pKa = 3.86TGVPLHH19 pKa = 7.18DD20 pKa = 4.08ISQVSYY26 pKa = 10.84LIEE29 pKa = 4.13LSIGTPGQSVKK40 pKa = 10.7VAVDD44 pKa = 3.52TGSSEE49 pKa = 4.32LWVDD53 pKa = 4.05PVCQTSQSLSEE64 pKa = 4.27AEE66 pKa = 3.79EE67 pKa = 4.75CIANGIYY74 pKa = 10.38DD75 pKa = 4.29PSRR78 pKa = 11.84SDD80 pKa = 3.04TFEE83 pKa = 4.92DD84 pKa = 3.92LNSTNTIPYY93 pKa = 9.95GIGAVQIEE101 pKa = 4.57YY102 pKa = 10.79VSDD105 pKa = 4.15NIAFPDD111 pKa = 3.58STINLTDD118 pKa = 3.28VQFGVATASRR128 pKa = 11.84QLNEE132 pKa = 4.58GIMGLSFGGNTPEE145 pKa = 5.82ADD147 pKa = 3.15LSYY150 pKa = 11.6NNIVDD155 pKa = 4.0EE156 pKa = 5.73LYY158 pKa = 10.8LQNATQSRR166 pKa = 11.84AFSVALGSSDD176 pKa = 3.65SDD178 pKa = 3.51NDD180 pKa = 3.6SVLIFGGIDD189 pKa = 3.1TSKK192 pKa = 10.49YY193 pKa = 10.15AGPLRR198 pKa = 11.84TLPILGPQNGEE209 pKa = 3.61TIYY212 pKa = 10.5RR213 pKa = 11.84YY214 pKa = 8.57WVQLDD219 pKa = 4.07SIGATVDD226 pKa = 3.99GSSHH230 pKa = 6.46TYY232 pKa = 10.38DD233 pKa = 3.31SSSLPVVLDD242 pKa = 3.52TGSTFCSLPRR252 pKa = 11.84AVVNGMMEE260 pKa = 4.5DD261 pKa = 3.78LDD263 pKa = 4.16GQIDD267 pKa = 3.67RR268 pKa = 11.84QGQVLVDD275 pKa = 3.87CSQVDD280 pKa = 3.54NEE282 pKa = 4.7DD283 pKa = 3.38TFDD286 pKa = 4.12FDD288 pKa = 4.88FGNITIRR295 pKa = 11.84IPYY298 pKa = 8.82SQFVVQANSEE308 pKa = 4.45VCVLGAIPDD317 pKa = 3.86DD318 pKa = 4.26QIALLGDD325 pKa = 3.49SFLRR329 pKa = 11.84SAYY332 pKa = 10.54VVFDD336 pKa = 3.8QNNMEE341 pKa = 4.5IAMAPYY347 pKa = 10.05ANCGQSEE354 pKa = 3.96QRR356 pKa = 11.84IPDD359 pKa = 3.52EE360 pKa = 4.36GVSGVEE366 pKa = 4.34GACDD370 pKa = 3.28SANNDD375 pKa = 3.22GGGDD379 pKa = 3.55SGGGDD384 pKa = 3.85DD385 pKa = 4.6NAGSRR390 pKa = 11.84LTASMLGLLSACISVPVVVDD410 pKa = 3.78LLL412 pKa = 3.95

Molecular weight: 43.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4U5B3|A0A4Q4U5B3_9PEZI MICOS complex subunit MIC60 OS=Monosporascus sp. MG133 OX=2211645 GN=DL767_010748 PE=3 SV=1
MM1 pKa = 7.62AKK3 pKa = 10.3ASSTVTTGRR12 pKa = 11.84TVTAGRR18 pKa = 11.84MATAHH23 pKa = 6.52HH24 pKa = 6.79GPPGRR29 pKa = 11.84HH30 pKa = 5.17MKK32 pKa = 10.79LPIQVAFAMGRR43 pKa = 11.84TKK45 pKa = 10.6VGSKK49 pKa = 11.01AMVDD53 pKa = 3.2NKK55 pKa = 9.8VRR57 pKa = 11.84AEE59 pKa = 3.79NRR61 pKa = 11.84AKK63 pKa = 10.4GRR65 pKa = 11.84SRR67 pKa = 11.84ARR69 pKa = 11.84TSHH72 pKa = 6.59LPMVRR77 pKa = 11.84RR78 pKa = 11.84LWWSLAPQGLRR89 pKa = 11.84LRR91 pKa = 11.84LLSKK95 pKa = 10.36PLAPSPITATTVVTMTRR112 pKa = 11.84RR113 pKa = 11.84TT114 pKa = 3.46

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11566

0

11566

5736655

66

8424

496.0

54.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.248 ± 0.023

1.177 ± 0.008

5.912 ± 0.017

6.342 ± 0.021

3.543 ± 0.015

7.305 ± 0.022

2.341 ± 0.009

4.514 ± 0.015

4.589 ± 0.021

8.759 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.009

3.505 ± 0.013

6.213 ± 0.031

3.856 ± 0.015

6.584 ± 0.019

7.835 ± 0.023

5.778 ± 0.015

6.212 ± 0.017

1.47 ± 0.009

2.712 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski