Amino acid dipepetide frequency for Monosporascus sp. MG133

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.868AlaAla: 10.868 ± 0.071	1.088AlaCys: 1.088 ± 0.014	4.859AlaAsp: 4.859 ± 0.031	5.818AlaGlu: 5.818 ± 0.047	3.168AlaPhe: 3.168 ± 0.029	6.64AlaGly: 6.64 ± 0.042	1.87AlaHis: 1.87 ± 0.02	4.103AlaIle: 4.103 ± 0.031	4.13AlaLys: 4.13 ± 0.032	8.076AlaLeu: 8.076 ± 0.046	2.027AlaMet: 2.027 ± 0.02	3.022AlaAsn: 3.022 ± 0.023	5.206AlaPro: 5.206 ± 0.045	3.44AlaGln: 3.44 ± 0.029	5.729AlaArg: 5.729 ± 0.034	7.412AlaSer: 7.412 ± 0.044	5.528AlaThr: 5.528 ± 0.033	5.981AlaVal: 5.981 ± 0.036	1.222AlaTrp: 1.222 ± 0.016	2.289AlaTyr: 2.289 ± 0.02	0.0AlaXaa: 0.0 ± 0.0
Cys	0.969CysAla: 0.969 ± 0.015	0.222CysCys: 0.222 ± 0.009	0.655CysAsp: 0.655 ± 0.01	0.605CysGlu: 0.605 ± 0.01	0.497CysPhe: 0.497 ± 0.011	0.961CysGly: 0.961 ± 0.015	0.309CysHis: 0.309 ± 0.008	0.642CysIle: 0.642 ± 0.011	0.461CysLys: 0.461 ± 0.011	1.162CysLeu: 1.162 ± 0.016	0.249CysMet: 0.249 ± 0.007	0.404CysAsn: 0.404 ± 0.009	0.631CysPro: 0.631 ± 0.011	0.415CysGln: 0.415 ± 0.009	0.799CysArg: 0.799 ± 0.013	0.823CysSer: 0.823 ± 0.013	0.634CysThr: 0.634 ± 0.011	0.789CysVal: 0.789 ± 0.012	0.197CysTrp: 0.197 ± 0.006	0.345CysTyr: 0.345 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	5.321AspAla: 5.321 ± 0.033	0.616AspCys: 0.616 ± 0.011	4.668AspAsp: 4.668 ± 0.048	4.699AspGlu: 4.699 ± 0.041	2.186AspPhe: 2.186 ± 0.021	4.646AspGly: 4.646 ± 0.033	1.235AspHis: 1.235 ± 0.012	2.974AspIle: 2.974 ± 0.025	2.349AspLys: 2.349 ± 0.027	5.077AspLeu: 5.077 ± 0.034	1.294AspMet: 1.294 ± 0.018	1.897AspAsn: 1.897 ± 0.02	3.602AspPro: 3.602 ± 0.025	1.792AspGln: 1.792 ± 0.017	3.343AspArg: 3.343 ± 0.031	4.023AspSer: 4.023 ± 0.029	2.87AspThr: 2.87 ± 0.025	3.935AspVal: 3.935 ± 0.024	0.928AspTrp: 0.928 ± 0.013	1.665AspTyr: 1.665 ± 0.017	0.0AspXaa: 0.0 ± 0.0
Glu	6.032GluAla: 6.032 ± 0.046	0.641GluCys: 0.641 ± 0.012	4.438GluAsp: 4.438 ± 0.037	5.573GluGlu: 5.573 ± 0.059	1.974GluPhe: 1.974 ± 0.021	4.083GluGly: 4.083 ± 0.031	1.402GluHis: 1.402 ± 0.016	2.94GluIle: 2.94 ± 0.021	3.56GluLys: 3.56 ± 0.035	5.424GluLeu: 5.424 ± 0.039	1.417GluMet: 1.417 ± 0.016	2.258GluAsn: 2.258 ± 0.022	3.052GluPro: 3.052 ± 0.037	2.454GluGln: 2.454 ± 0.026	4.279GluArg: 4.279 ± 0.033	4.146GluSer: 4.146 ± 0.035	3.467GluThr: 3.467 ± 0.027	3.723GluVal: 3.723 ± 0.029	0.913GluTrp: 0.913 ± 0.012	1.647GluTyr: 1.647 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.078PheAla: 3.078 ± 0.029	0.512PheCys: 0.512 ± 0.011	2.247PheAsp: 2.247 ± 0.021	2.198PheGlu: 2.198 ± 0.02	1.469PhePhe: 1.469 ± 0.018	2.85PheGly: 2.85 ± 0.025	0.826PheHis: 0.826 ± 0.012	1.582PheIle: 1.582 ± 0.02	1.436PheLys: 1.436 ± 0.018	3.219PheLeu: 3.219 ± 0.028	0.745PheMet: 0.745 ± 0.011	1.282PheAsn: 1.282 ± 0.016	1.854PhePro: 1.854 ± 0.019	1.29PheGln: 1.29 ± 0.016	2.107PheArg: 2.107 ± 0.022	2.711PheSer: 2.711 ± 0.025	2.001PheThr: 2.001 ± 0.02	2.348PheVal: 2.348 ± 0.026	0.626PheTrp: 0.626 ± 0.011	1.054PheTyr: 1.054 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	6.228GlyAla: 6.228 ± 0.039	0.888GlyCys: 0.888 ± 0.014	4.239GlyAsp: 4.239 ± 0.031	3.981GlyGlu: 3.981 ± 0.03	2.784GlyPhe: 2.784 ± 0.025	6.839GlyGly: 6.839 ± 0.057	1.836GlyHis: 1.836 ± 0.023	3.424GlyIle: 3.424 ± 0.027	3.366GlyLys: 3.366 ± 0.027	6.126GlyLeu: 6.126 ± 0.035	1.55GlyMet: 1.55 ± 0.018	2.645GlyAsn: 2.645 ± 0.025	3.792GlyPro: 3.792 ± 0.037	2.552GlyGln: 2.552 ± 0.024	4.827GlyArg: 4.827 ± 0.04	5.908GlySer: 5.908 ± 0.042	4.147GlyThr: 4.147 ± 0.032	4.58GlyVal: 4.58 ± 0.034	1.195GlyTrp: 1.195 ± 0.016	2.146GlyTyr: 2.146 ± 0.021	0.0GlyXaa: 0.0 ± 0.0
His	1.913HisAla: 1.913 ± 0.022	0.306HisCys: 0.306 ± 0.008	1.313HisAsp: 1.313 ± 0.017	1.34HisGlu: 1.34 ± 0.018	0.88HisPhe: 0.88 ± 0.013	1.8HisGly: 1.8 ± 0.02	0.883HisHis: 0.883 ± 0.016	1.122HisIle: 1.122 ± 0.015	0.852HisLys: 0.852 ± 0.011	2.129HisLeu: 2.129 ± 0.02	0.487HisMet: 0.487 ± 0.009	0.808HisAsn: 0.808 ± 0.013	1.668HisPro: 1.668 ± 0.021	0.974HisGln: 0.974 ± 0.013	1.574HisArg: 1.574 ± 0.02	1.69HisSer: 1.69 ± 0.022	1.159HisThr: 1.159 ± 0.016	1.475HisVal: 1.475 ± 0.015	0.337HisTrp: 0.337 ± 0.008	0.697HisTyr: 0.697 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.002IleAla: 4.002 ± 0.029	0.681IleCys: 0.681 ± 0.013	2.741IleAsp: 2.741 ± 0.023	2.727IleGlu: 2.727 ± 0.025	1.757IlePhe: 1.757 ± 0.018	2.966IleGly: 2.966 ± 0.026	1.069IleHis: 1.069 ± 0.015	2.193IleIle: 2.193 ± 0.025	1.989IleLys: 1.989 ± 0.022	4.129IleLeu: 4.129 ± 0.031	0.944IleMet: 0.944 ± 0.014	1.624IleAsn: 1.624 ± 0.018	2.828IlePro: 2.828 ± 0.024	1.687IleGln: 1.687 ± 0.018	2.843IleArg: 2.843 ± 0.022	3.391IleSer: 3.391 ± 0.028	2.619IleThr: 2.619 ± 0.024	2.961IleVal: 2.961 ± 0.026	0.692IleTrp: 0.692 ± 0.013	1.3IleTyr: 1.3 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	4.309LysAla: 4.309 ± 0.029	0.43LysCys: 0.43 ± 0.009	2.683LysAsp: 2.683 ± 0.028	3.217LysGlu: 3.217 ± 0.032	1.359LysPhe: 1.359 ± 0.018	3.01LysGly: 3.01 ± 0.028	1.034LysHis: 1.034 ± 0.015	2.003LysIle: 2.003 ± 0.019	3.123LysLys: 3.123 ± 0.042	3.912LysLeu: 3.912 ± 0.029	0.931LysMet: 0.931 ± 0.011	1.574LysAsn: 1.574 ± 0.017	2.716LysPro: 2.716 ± 0.026	1.748LysGln: 1.748 ± 0.021	3.33LysArg: 3.33 ± 0.03	3.191LysSer: 3.191 ± 0.029	2.678LysThr: 2.678 ± 0.024	2.732LysVal: 2.732 ± 0.025	0.632LysTrp: 0.632 ± 0.01	1.277LysTyr: 1.277 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	8.215LeuAla: 8.215 ± 0.041	1.146LeuCys: 1.146 ± 0.016	5.208LeuAsp: 5.208 ± 0.035	5.619LeuGlu: 5.619 ± 0.039	3.118LeuPhe: 3.118 ± 0.03	6.114LeuGly: 6.114 ± 0.04	2.096LeuHis: 2.096 ± 0.02	3.532LeuIle: 3.532 ± 0.03	4.004LeuLys: 4.004 ± 0.028	8.255LeuLeu: 8.255 ± 0.06	1.74LeuMet: 1.74 ± 0.018	2.938LeuAsn: 2.938 ± 0.024	5.445LeuPro: 5.445 ± 0.034	3.607LeuGln: 3.607 ± 0.03	6.26LeuArg: 6.26 ± 0.035	6.81LeuSer: 6.81 ± 0.04	4.579LeuThr: 4.579 ± 0.037	5.44LeuVal: 5.44 ± 0.039	1.224LeuTrp: 1.224 ± 0.018	2.234LeuTyr: 2.234 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	2.269MetAla: 2.269 ± 0.019	0.233MetCys: 0.233 ± 0.006	1.233MetAsp: 1.233 ± 0.016	1.249MetGlu: 1.249 ± 0.014	0.704MetPhe: 0.704 ± 0.012	1.43MetGly: 1.43 ± 0.016	0.471MetHis: 0.471 ± 0.008	0.89MetIle: 0.89 ± 0.014	0.945MetLys: 0.945 ± 0.013	1.798MetLeu: 1.798 ± 0.019	0.555MetMet: 0.555 ± 0.012	0.743MetAsn: 0.743 ± 0.011	1.291MetPro: 1.291 ± 0.017	0.811MetGln: 0.811 ± 0.013	1.368MetArg: 1.368 ± 0.014	1.725MetSer: 1.725 ± 0.018	1.268MetThr: 1.268 ± 0.016	1.297MetVal: 1.297 ± 0.016	0.262MetTrp: 0.262 ± 0.007	0.505MetTyr: 0.505 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	3.138AsnAla: 3.138 ± 0.022	0.418AsnCys: 0.418 ± 0.009	1.881AsnAsp: 1.881 ± 0.02	1.93AsnGlu: 1.93 ± 0.021	1.263AsnPhe: 1.263 ± 0.014	3.079AsnGly: 3.079 ± 0.028	0.811AsnHis: 0.811 ± 0.014	1.817AsnIle: 1.817 ± 0.017	1.429AsnLys: 1.429 ± 0.018	3.062AsnLeu: 3.062 ± 0.022	0.789AsnMet: 0.789 ± 0.012	1.502AsnAsn: 1.502 ± 0.022	2.431AsnPro: 2.431 ± 0.022	1.228AsnGln: 1.228 ± 0.015	1.997AsnArg: 1.997 ± 0.02	2.558AsnSer: 2.558 ± 0.025	2.003AsnThr: 2.003 ± 0.022	2.187AsnVal: 2.187 ± 0.018	0.534AsnTrp: 0.534 ± 0.012	0.994AsnTyr: 0.994 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	5.682ProAla: 5.682 ± 0.048	0.511ProCys: 0.511 ± 0.01	3.517ProAsp: 3.517 ± 0.027	4.126ProGlu: 4.126 ± 0.034	1.992ProPhe: 1.992 ± 0.019	4.492ProGly: 4.492 ± 0.039	1.327ProHis: 1.327 ± 0.017	2.316ProIle: 2.316 ± 0.022	2.611ProLys: 2.611 ± 0.024	4.811ProLeu: 4.811 ± 0.033	1.098ProMet: 1.098 ± 0.016	2.162ProAsn: 2.162 ± 0.022	5.581ProPro: 5.581 ± 0.075	2.47ProGln: 2.47 ± 0.029	3.801ProArg: 3.801 ± 0.034	5.764ProSer: 5.764 ± 0.048	3.953ProThr: 3.953 ± 0.035	3.632ProVal: 3.632 ± 0.03	0.777ProTrp: 0.777 ± 0.011	1.507ProTyr: 1.507 ± 0.017	0.0ProXaa: 0.0 ± 0.0
Gln	3.507GlnAla: 3.507 ± 0.03	0.417GlnCys: 0.417 ± 0.01	2.006GlnAsp: 2.006 ± 0.021	2.363GlnGlu: 2.363 ± 0.024	1.198GlnPhe: 1.198 ± 0.015	2.438GlnGly: 2.438 ± 0.025	1.048GlnHis: 1.048 ± 0.018	1.704GlnIle: 1.704 ± 0.019	1.843GlnLys: 1.843 ± 0.021	3.319GlnLeu: 3.319 ± 0.028	0.81GlnMet: 0.81 ± 0.013	1.38GlnAsn: 1.38 ± 0.016	2.581GlnPro: 2.581 ± 0.032	2.543GlnGln: 2.543 ± 0.042	2.664GlnArg: 2.664 ± 0.022	2.812GlnSer: 2.812 ± 0.025	2.146GlnThr: 2.146 ± 0.02	2.125GlnVal: 2.125 ± 0.025	0.559GlnTrp: 0.559 ± 0.011	1.1GlnTyr: 1.1 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	5.466ArgAla: 5.466 ± 0.036	0.759ArgCys: 0.759 ± 0.013	3.917ArgAsp: 3.917 ± 0.033	4.217ArgGlu: 4.217 ± 0.033	2.216ArgPhe: 2.216 ± 0.023	4.384ArgGly: 4.384 ± 0.037	1.746ArgHis: 1.746 ± 0.019	2.922ArgIle: 2.922 ± 0.023	3.356ArgLys: 3.356 ± 0.025	5.935ArgLeu: 5.935 ± 0.041	1.377ArgMet: 1.377 ± 0.015	2.363ArgAsn: 2.363 ± 0.021	3.906ArgPro: 3.906 ± 0.028	2.733ArgGln: 2.733 ± 0.022	5.898ArgArg: 5.898 ± 0.052	4.818ArgSer: 4.818 ± 0.038	3.422ArgThr: 3.422 ± 0.024	3.671ArgVal: 3.671 ± 0.027	1.026ArgTrp: 1.026 ± 0.014	1.708ArgTyr: 1.708 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	6.793SerAla: 6.793 ± 0.04	0.805SerCys: 0.805 ± 0.013	4.126SerAsp: 4.126 ± 0.033	4.115SerGlu: 4.115 ± 0.028	2.761SerPhe: 2.761 ± 0.022	5.747SerGly: 5.747 ± 0.041	1.799SerHis: 1.799 ± 0.021	3.462SerIle: 3.462 ± 0.028	3.376SerLys: 3.376 ± 0.031	6.634SerLeu: 6.634 ± 0.038	1.605SerMet: 1.605 ± 0.018	2.706SerAsn: 2.706 ± 0.023	5.405SerPro: 5.405 ± 0.053	3.031SerGln: 3.031 ± 0.028	5.157SerArg: 5.157 ± 0.042	8.237SerSer: 8.237 ± 0.062	5.078SerThr: 5.078 ± 0.042	4.384SerVal: 4.384 ± 0.031	1.12SerTrp: 1.12 ± 0.016	2.012SerTyr: 2.012 ± 0.018	0.0SerXaa: 0.0 ± 0.0
Thr	5.525ThrAla: 5.525 ± 0.037	0.712ThrCys: 0.712 ± 0.013	2.791ThrAsp: 2.791 ± 0.022	3.127ThrGlu: 3.127 ± 0.028	2.062ThrPhe: 2.062 ± 0.019	4.251ThrGly: 4.251 ± 0.033	1.187ThrHis: 1.187 ± 0.017	2.751ThrIle: 2.751 ± 0.023	2.432ThrLys: 2.432 ± 0.02	4.91ThrLeu: 4.91 ± 0.035	1.181ThrMet: 1.181 ± 0.016	1.926ThrAsn: 1.926 ± 0.023	4.286ThrPro: 4.286 ± 0.035	1.963ThrGln: 1.963 ± 0.019	3.275ThrArg: 3.275 ± 0.024	4.974ThrSer: 4.974 ± 0.037	4.311ThrThr: 4.311 ± 0.048	3.72ThrVal: 3.72 ± 0.033	0.846ThrTrp: 0.846 ± 0.012	1.553ThrTyr: 1.553 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	5.695ValAla: 5.695 ± 0.039	0.796ValCys: 0.796 ± 0.014	3.89ValAsp: 3.89 ± 0.028	4.023ValGlu: 4.023 ± 0.031	2.379ValPhe: 2.379 ± 0.022	4.175ValGly: 4.175 ± 0.034	1.369ValHis: 1.369 ± 0.016	2.826ValIle: 2.826 ± 0.025	2.84ValLys: 2.84 ± 0.026	5.667ValLeu: 5.667 ± 0.035	1.292ValMet: 1.292 ± 0.015	2.175ValAsn: 2.175 ± 0.02	3.758ValPro: 3.758 ± 0.03	2.276ValGln: 2.276 ± 0.02	3.809ValArg: 3.809 ± 0.028	4.47ValSer: 4.47 ± 0.034	3.533ValThr: 3.533 ± 0.029	4.514ValVal: 4.514 ± 0.035	0.886ValTrp: 0.886 ± 0.013	1.742ValTyr: 1.742 ± 0.017	0.0ValXaa: 0.0 ± 0.0
Trp	1.238TrpAla: 1.238 ± 0.017	0.19TrpCys: 0.19 ± 0.005	0.98TrpAsp: 0.98 ± 0.015	0.877TrpGlu: 0.877 ± 0.013	0.544TrpPhe: 0.544 ± 0.011	0.984TrpGly: 0.984 ± 0.014	0.366TrpHis: 0.366 ± 0.009	0.717TrpIle: 0.717 ± 0.012	0.736TrpLys: 0.736 ± 0.011	1.37TrpLeu: 1.37 ± 0.016	0.357TrpMet: 0.357 ± 0.008	0.606TrpAsn: 0.606 ± 0.011	0.631TrpPro: 0.631 ± 0.012	0.539TrpGln: 0.539 ± 0.011	1.061TrpArg: 1.061 ± 0.015	0.99TrpSer: 0.99 ± 0.014	0.888TrpThr: 0.888 ± 0.015	0.928TrpVal: 0.928 ± 0.014	0.271TrpTrp: 0.271 ± 0.007	0.424TrpTyr: 0.424 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.229TyrAla: 2.229 ± 0.022	0.397TyrCys: 0.397 ± 0.01	1.729TyrAsp: 1.729 ± 0.019	1.618TyrGlu: 1.618 ± 0.016	1.122TyrPhe: 1.122 ± 0.014	2.166TyrGly: 2.166 ± 0.022	0.723TyrHis: 0.723 ± 0.012	1.224TyrIle: 1.224 ± 0.014	1.05TyrLys: 1.05 ± 0.015	2.57TyrLeu: 2.57 ± 0.025	0.601TyrMet: 0.601 ± 0.01	1.036TyrAsn: 1.036 ± 0.015	1.468TyrPro: 1.468 ± 0.019	1.009TyrGln: 1.009 ± 0.016	1.721TyrArg: 1.721 ± 0.019	1.898TyrSer: 1.898 ± 0.021	1.498TyrThr: 1.498 ± 0.016	1.693TyrVal: 1.693 ± 0.018	0.452TyrTrp: 0.452 ± 0.01	0.917TyrTyr: 0.917 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11566 proteins (5736655 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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