Halicephalobus sp. NKZ332

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Tylenchina; Panagrolaimomorpha; Panagrolaimoidea; Panagrolaimidae; Halicephalobus; unclassified Halicephalobus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10967 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G0V4C9|A0A6G0V4C9_9BILA Protein kinase domain-containing protein OS=Halicephalobus sp. NKZ332 OX=2598192 GN=FO519_001404 PE=3 SV=1
MM1 pKa = 7.45LKK3 pKa = 10.3VLLFSCSIAFFLTVGSSQFTTPDD26 pKa = 3.36ALPTQDD32 pKa = 4.04VATEE36 pKa = 4.27YY37 pKa = 10.07PGSSSSQPMSTGEE50 pKa = 3.89FTQTQGFSPTGEE62 pKa = 4.11STQTQGFSPTGEE74 pKa = 4.11STQTQGFSPTGEE86 pKa = 4.11STQTQGFSPTGEE98 pKa = 4.06STQTIVSDD106 pKa = 3.62TTPGGNPSPSGNCNGIIFIQEE127 pKa = 3.54MTQAISDD134 pKa = 4.05SEE136 pKa = 4.02KK137 pKa = 10.93SNFFGAVKK145 pKa = 10.26KK146 pKa = 10.3IAASVSSSLQFGFFQFGNVLYY167 pKa = 10.77GSLQLTDD174 pKa = 3.67YY175 pKa = 9.81NTFLGNVNAAEE186 pKa = 4.27ANFNSSNQPDD196 pKa = 3.93GSSAYY201 pKa = 9.29IAPPLNEE208 pKa = 5.1LLNTLNNNPGSRR220 pKa = 11.84FAVLLGAEE228 pKa = 4.33QEE230 pKa = 4.42NIRR233 pKa = 11.84DD234 pKa = 3.79PAQGNTVSNNIASAGIDD251 pKa = 3.34VYY253 pKa = 11.7VFDD256 pKa = 4.64YY257 pKa = 10.97SIGIIPFSLWNTLTGKK273 pKa = 10.37APNHH277 pKa = 6.33VINGTDD283 pKa = 3.09QTADD287 pKa = 3.22QLFNSLQSTLINDD300 pKa = 3.83VNNIGCC306 pKa = 4.35

Molecular weight: 32.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G0UAJ8|A0A6G0UAJ8_9BILA Uncharacterized protein (Fragment) OS=Halicephalobus sp. NKZ332 OX=2598192 GN=FO519_010650 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 7.83TTPKK6 pKa = 10.66LKK8 pKa = 10.44LLPNKK13 pKa = 9.54RR14 pKa = 11.84QRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84LLKK21 pKa = 9.2TRR23 pKa = 11.84PRR25 pKa = 11.84SKK27 pKa = 10.8LLPNKK32 pKa = 9.81RR33 pKa = 11.84LRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84WKK39 pKa = 10.05RR40 pKa = 11.84RR41 pKa = 11.84NLKK44 pKa = 9.72RR45 pKa = 11.84KK46 pKa = 8.56KK47 pKa = 9.37SRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 9.74LRR54 pKa = 11.84LKK56 pKa = 10.38LLPNKK61 pKa = 9.53RR62 pKa = 11.84QRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84LLKK69 pKa = 9.33TRR71 pKa = 11.84PRR73 pKa = 11.84LKK75 pKa = 10.58LLPNKK80 pKa = 10.15RR81 pKa = 11.84LRR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84LKK87 pKa = 10.39RR88 pKa = 11.84RR89 pKa = 11.84NLKK92 pKa = 9.91KK93 pKa = 10.4KK94 pKa = 9.75KK95 pKa = 9.48SRR97 pKa = 11.84RR98 pKa = 3.65

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10967

0

10967

5512870

27

8003

502.7

56.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.883 ± 0.017

1.926 ± 0.02

5.454 ± 0.016

6.878 ± 0.036

4.798 ± 0.019

5.709 ± 0.027

2.12 ± 0.009

6.286 ± 0.019

6.516 ± 0.026

8.727 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.011

5.132 ± 0.016

5.166 ± 0.029

3.967 ± 0.019

5.06 ± 0.017

7.943 ± 0.025

5.505 ± 0.019

6.269 ± 0.018

1.067 ± 0.006

3.241 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski