Amino acid dipepetide frequency for Halicephalobus sp. NKZ332

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.614AlaAla: 4.614 ± 0.049	1.039AlaCys: 1.039 ± 0.018	3.032AlaAsp: 3.032 ± 0.027	3.859AlaGlu: 3.859 ± 0.043	2.781AlaPhe: 2.781 ± 0.024	3.327AlaGly: 3.327 ± 0.032	1.153AlaHis: 1.153 ± 0.018	3.815AlaIle: 3.815 ± 0.032	3.579AlaLys: 3.579 ± 0.036	5.468AlaLeu: 5.468 ± 0.043	1.445AlaMet: 1.445 ± 0.019	2.62AlaAsn: 2.62 ± 0.025	2.873AlaPro: 2.873 ± 0.045	2.259AlaGln: 2.259 ± 0.026	2.741AlaArg: 2.741 ± 0.024	4.473AlaSer: 4.473 ± 0.037	3.284AlaThr: 3.284 ± 0.031	4.198AlaVal: 4.198 ± 0.033	0.541AlaTrp: 0.541 ± 0.01	1.724AlaTyr: 1.724 ± 0.018	0.0AlaXaa: 0.0 ± 0.0
Cys	1.016CysAla: 1.016 ± 0.016	0.551CysCys: 0.551 ± 0.018	1.055CysAsp: 1.055 ± 0.024	1.112CysGlu: 1.112 ± 0.023	0.963CysPhe: 0.963 ± 0.015	1.319CysGly: 1.319 ± 0.026	0.472CysHis: 0.472 ± 0.01	1.135CysIle: 1.135 ± 0.022	1.03CysLys: 1.03 ± 0.018	1.647CysLeu: 1.647 ± 0.022	0.395CysMet: 0.395 ± 0.009	0.94CysAsn: 0.94 ± 0.018	1.169CysPro: 1.169 ± 0.038	0.829CysGln: 0.829 ± 0.02	1.035CysArg: 1.035 ± 0.018	1.562CysSer: 1.562 ± 0.027	0.981CysThr: 0.981 ± 0.019	1.201CysVal: 1.201 ± 0.021	0.214CysTrp: 0.214 ± 0.006	0.636CysTyr: 0.636 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	2.894AspAla: 2.894 ± 0.027	1.028AspCys: 1.028 ± 0.022	3.6AspAsp: 3.6 ± 0.034	4.644AspGlu: 4.644 ± 0.041	2.94AspPhe: 2.94 ± 0.028	3.403AspGly: 3.403 ± 0.043	1.136AspHis: 1.136 ± 0.012	3.245AspIle: 3.245 ± 0.024	2.952AspLys: 2.952 ± 0.029	4.88AspLeu: 4.88 ± 0.039	1.099AspMet: 1.099 ± 0.014	2.332AspAsn: 2.332 ± 0.023	2.956AspPro: 2.956 ± 0.028	1.99AspGln: 1.99 ± 0.019	2.515AspArg: 2.515 ± 0.023	4.396AspSer: 4.396 ± 0.033	2.425AspThr: 2.425 ± 0.025	3.441AspVal: 3.441 ± 0.028	0.65AspTrp: 0.65 ± 0.012	2.017AspTyr: 2.017 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	4.109GluAla: 4.109 ± 0.046	1.078GluCys: 1.078 ± 0.022	4.152GluAsp: 4.152 ± 0.033	6.394GluGlu: 6.394 ± 0.068	3.013GluPhe: 3.013 ± 0.024	3.102GluGly: 3.102 ± 0.028	1.317GluHis: 1.317 ± 0.016	4.504GluIle: 4.504 ± 0.03	6.016GluLys: 6.016 ± 0.059	5.737GluLeu: 5.737 ± 0.053	1.64GluMet: 1.64 ± 0.02	4.094GluAsn: 4.094 ± 0.031	2.521GluPro: 2.521 ± 0.03	2.507GluGln: 2.507 ± 0.03	3.535GluArg: 3.535 ± 0.039	4.876GluSer: 4.876 ± 0.042	3.456GluThr: 3.456 ± 0.052	3.986GluVal: 3.986 ± 0.034	0.674GluTrp: 0.674 ± 0.012	2.074GluTyr: 2.074 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	2.82PheAla: 2.82 ± 0.025	1.058PheCys: 1.058 ± 0.017	2.86PheAsp: 2.86 ± 0.025	3.0PheGlu: 3.0 ± 0.023	2.543PhePhe: 2.543 ± 0.03	3.228PheGly: 3.228 ± 0.032	1.096PheHis: 1.096 ± 0.015	3.021PheIle: 3.021 ± 0.029	2.472PheLys: 2.472 ± 0.025	4.47PheLeu: 4.47 ± 0.037	1.061PheMet: 1.061 ± 0.013	2.339PheAsn: 2.339 ± 0.025	2.178PhePro: 2.178 ± 0.023	1.672PheGln: 1.672 ± 0.018	2.172PheArg: 2.172 ± 0.024	3.767PheSer: 3.767 ± 0.029	2.512PheThr: 2.512 ± 0.027	3.338PheVal: 3.338 ± 0.027	0.607PheTrp: 0.607 ± 0.013	1.765PheTyr: 1.765 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	3.205GlyAla: 3.205 ± 0.029	1.089GlyCys: 1.089 ± 0.023	2.96GlyAsp: 2.96 ± 0.032	3.32GlyGlu: 3.32 ± 0.039	3.103GlyPhe: 3.103 ± 0.033	3.814GlyGly: 3.814 ± 0.062	1.277GlyHis: 1.277 ± 0.02	3.919GlyIle: 3.919 ± 0.029	3.685GlyLys: 3.685 ± 0.036	4.569GlyLeu: 4.569 ± 0.048	1.305GlyMet: 1.305 ± 0.019	3.116GlyAsn: 3.116 ± 0.032	2.48GlyPro: 2.48 ± 0.041	2.105GlyGln: 2.105 ± 0.028	2.792GlyArg: 2.792 ± 0.031	4.751GlySer: 4.751 ± 0.046	3.313GlyThr: 3.313 ± 0.036	3.454GlyVal: 3.454 ± 0.032	0.635GlyTrp: 0.635 ± 0.012	2.195GlyTyr: 2.195 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	1.069HisAla: 1.069 ± 0.016	0.521HisCys: 0.521 ± 0.012	1.023HisAsp: 1.023 ± 0.015	1.298HisGlu: 1.298 ± 0.016	1.11HisPhe: 1.11 ± 0.016	1.333HisGly: 1.333 ± 0.016	0.663HisHis: 0.663 ± 0.013	1.254HisIle: 1.254 ± 0.016	1.104HisLys: 1.104 ± 0.013	2.007HisLeu: 2.007 ± 0.021	0.498HisMet: 0.498 ± 0.009	0.946HisAsn: 0.946 ± 0.016	1.214HisPro: 1.214 ± 0.017	0.95HisGln: 0.95 ± 0.014	1.228HisArg: 1.228 ± 0.017	1.638HisSer: 1.638 ± 0.02	0.936HisThr: 0.936 ± 0.015	1.331HisVal: 1.331 ± 0.015	0.284HisTrp: 0.284 ± 0.008	0.791HisTyr: 0.791 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	3.852IleAla: 3.852 ± 0.033	1.399IleCys: 1.399 ± 0.021	3.59IleAsp: 3.59 ± 0.028	4.045IleGlu: 4.045 ± 0.035	3.194IlePhe: 3.194 ± 0.033	3.561IleGly: 3.561 ± 0.032	1.493IleHis: 1.493 ± 0.017	3.779IleIle: 3.779 ± 0.035	3.481IleLys: 3.481 ± 0.027	5.714IleLeu: 5.714 ± 0.046	1.306IleMet: 1.306 ± 0.017	2.943IleAsn: 2.943 ± 0.024	3.579IlePro: 3.579 ± 0.026	2.67IleGln: 2.67 ± 0.025	3.064IleArg: 3.064 ± 0.024	5.027IleSer: 5.027 ± 0.035	3.305IleThr: 3.305 ± 0.028	4.069IleVal: 4.069 ± 0.034	0.701IleTrp: 0.701 ± 0.012	2.091IleTyr: 2.091 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	3.585LysAla: 3.585 ± 0.036	1.169LysCys: 1.169 ± 0.021	3.5LysAsp: 3.5 ± 0.03	5.121LysGlu: 5.121 ± 0.05	2.801LysPhe: 2.801 ± 0.024	2.677LysGly: 2.677 ± 0.031	1.272LysHis: 1.272 ± 0.017	4.33LysIle: 4.33 ± 0.034	5.965LysLys: 5.965 ± 0.065	5.699LysLeu: 5.699 ± 0.044	1.602LysMet: 1.602 ± 0.022	3.752LysAsn: 3.752 ± 0.029	2.91LysPro: 2.91 ± 0.032	2.431LysGln: 2.431 ± 0.025	3.531LysArg: 3.531 ± 0.031	4.721LysSer: 4.721 ± 0.038	3.604LysThr: 3.604 ± 0.026	3.741LysVal: 3.741 ± 0.029	0.684LysTrp: 0.684 ± 0.012	2.061LysTyr: 2.061 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	5.413LeuAla: 5.413 ± 0.044	1.619LeuCys: 1.619 ± 0.021	4.636LeuAsp: 4.636 ± 0.03	5.77LeuGlu: 5.77 ± 0.049	4.083LeuPhe: 4.083 ± 0.037	4.545LeuGly: 4.545 ± 0.034	1.96LeuHis: 1.96 ± 0.024	5.473LeuIle: 5.473 ± 0.043	6.151LeuLys: 6.151 ± 0.043	8.126LeuLeu: 8.126 ± 0.066	2.17LeuMet: 2.17 ± 0.023	4.47LeuAsn: 4.47 ± 0.033	4.568LeuPro: 4.568 ± 0.035	3.6LeuGln: 3.6 ± 0.031	4.597LeuArg: 4.597 ± 0.037	6.657LeuSer: 6.657 ± 0.042	4.8LeuThr: 4.8 ± 0.032	5.16LeuVal: 5.16 ± 0.034	0.873LeuTrp: 0.873 ± 0.013	2.602LeuTyr: 2.602 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	1.544MetAla: 1.544 ± 0.019	0.376MetCys: 0.376 ± 0.009	1.413MetAsp: 1.413 ± 0.016	1.674MetGlu: 1.674 ± 0.02	0.992MetPhe: 0.992 ± 0.016	1.204MetGly: 1.204 ± 0.014	0.423MetHis: 0.423 ± 0.009	1.517MetIle: 1.517 ± 0.019	1.78MetLys: 1.78 ± 0.019	1.882MetLeu: 1.882 ± 0.023	0.738MetMet: 0.738 ± 0.013	1.417MetAsn: 1.417 ± 0.016	1.045MetPro: 1.045 ± 0.017	0.79MetGln: 0.79 ± 0.014	1.181MetArg: 1.181 ± 0.015	1.832MetSer: 1.832 ± 0.022	1.506MetThr: 1.506 ± 0.022	1.354MetVal: 1.354 ± 0.016	0.2MetTrp: 0.2 ± 0.007	0.641MetTyr: 0.641 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.795AsnAla: 2.795 ± 0.025	1.017AsnCys: 1.017 ± 0.017	2.646AsnAsp: 2.646 ± 0.025	3.406AsnGlu: 3.406 ± 0.027	2.613AsnPhe: 2.613 ± 0.027	3.534AsnGly: 3.534 ± 0.039	1.058AsnHis: 1.058 ± 0.016	3.135AsnIle: 3.135 ± 0.023	2.699AsnLys: 2.699 ± 0.026	4.474AsnLeu: 4.474 ± 0.032	1.191AsnMet: 1.191 ± 0.015	2.733AsnAsn: 2.733 ± 0.03	3.078AsnPro: 3.078 ± 0.03	2.148AsnGln: 2.148 ± 0.026	2.382AsnArg: 2.382 ± 0.02	4.4AsnSer: 4.4 ± 0.036	2.453AsnThr: 2.453 ± 0.026	3.108AsnVal: 3.108 ± 0.025	0.59AsnTrp: 0.59 ± 0.011	1.865AsnTyr: 1.865 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	2.779ProAla: 2.779 ± 0.036	0.744ProCys: 0.744 ± 0.02	2.673ProAsp: 2.673 ± 0.025	3.975ProGlu: 3.975 ± 0.035	2.075ProPhe: 2.075 ± 0.025	3.352ProGly: 3.352 ± 0.085	0.975ProHis: 0.975 ± 0.013	2.897ProIle: 2.897 ± 0.023	3.18ProLys: 3.18 ± 0.031	3.924ProLeu: 3.924 ± 0.033	1.153ProMet: 1.153 ± 0.016	2.508ProAsn: 2.508 ± 0.026	3.991ProPro: 3.991 ± 0.062	2.292ProGln: 2.292 ± 0.036	2.418ProArg: 2.418 ± 0.023	4.378ProSer: 4.378 ± 0.039	3.028ProThr: 3.028 ± 0.033	3.356ProVal: 3.356 ± 0.034	0.466ProTrp: 0.466 ± 0.01	1.494ProTyr: 1.494 ± 0.019	0.0ProXaa: 0.0 ± 0.0
Gln	2.224GlnAla: 2.224 ± 0.024	0.787GlnCys: 0.787 ± 0.021	1.638GlnAsp: 1.638 ± 0.018	2.463GlnGlu: 2.463 ± 0.028	1.729GlnPhe: 1.729 ± 0.017	1.948GlnGly: 1.948 ± 0.024	0.812GlnHis: 0.812 ± 0.013	2.554GlnIle: 2.554 ± 0.022	2.979GlnLys: 2.979 ± 0.031	3.808GlnLeu: 3.808 ± 0.035	1.149GlnMet: 1.149 ± 0.016	2.33GlnAsn: 2.33 ± 0.029	1.929GlnPro: 1.929 ± 0.034	2.36GlnGln: 2.36 ± 0.048	2.197GlnArg: 2.197 ± 0.021	2.747GlnSer: 2.747 ± 0.027	2.019GlnThr: 2.019 ± 0.025	2.302GlnVal: 2.302 ± 0.023	0.442GlnTrp: 0.442 ± 0.008	1.255GlnTyr: 1.255 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	2.726ArgAla: 2.726 ± 0.022	0.964ArgCys: 0.964 ± 0.019	2.501ArgAsp: 2.501 ± 0.026	3.249ArgGlu: 3.249 ± 0.026	2.364ArgPhe: 2.364 ± 0.026	2.469ArgGly: 2.469 ± 0.029	1.181ArgHis: 1.181 ± 0.016	3.354ArgIle: 3.354 ± 0.026	3.805ArgLys: 3.805 ± 0.033	4.32ArgLeu: 4.32 ± 0.03	1.218ArgMet: 1.218 ± 0.017	2.705ArgAsn: 2.705 ± 0.024	2.405ArgPro: 2.405 ± 0.024	2.204ArgGln: 2.204 ± 0.02	3.718ArgArg: 3.718 ± 0.037	3.755ArgSer: 3.755 ± 0.032	2.658ArgThr: 2.658 ± 0.023	2.756ArgVal: 2.756 ± 0.023	0.586ArgTrp: 0.586 ± 0.011	1.667ArgTyr: 1.667 ± 0.02	0.0ArgXaa: 0.0 ± 0.0
Ser	4.476SerAla: 4.476 ± 0.035	1.435SerCys: 1.435 ± 0.025	4.243SerAsp: 4.243 ± 0.032	5.139SerGlu: 5.139 ± 0.044	3.605SerPhe: 3.605 ± 0.027	5.078SerGly: 5.078 ± 0.046	1.519SerHis: 1.519 ± 0.021	4.799SerIle: 4.799 ± 0.033	4.751SerLys: 4.751 ± 0.034	6.529SerLeu: 6.529 ± 0.043	1.828SerMet: 1.828 ± 0.019	4.058SerAsn: 4.058 ± 0.033	4.239SerPro: 4.239 ± 0.043	3.001SerGln: 3.001 ± 0.028	3.909SerArg: 3.909 ± 0.032	7.861SerSer: 7.861 ± 0.073	5.137SerThr: 5.137 ± 0.048	4.833SerVal: 4.833 ± 0.032	0.786SerTrp: 0.786 ± 0.013	2.2SerTyr: 2.2 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	3.47ThrAla: 3.47 ± 0.027	1.143ThrCys: 1.143 ± 0.026	2.829ThrAsp: 2.829 ± 0.027	3.565ThrGlu: 3.565 ± 0.054	2.55ThrPhe: 2.55 ± 0.024	3.257ThrGly: 3.257 ± 0.029	0.993ThrHis: 0.993 ± 0.015	3.428ThrIle: 3.428 ± 0.034	3.199ThrLys: 3.199 ± 0.023	4.663ThrLeu: 4.663 ± 0.034	1.268ThrMet: 1.268 ± 0.018	2.631ThrAsn: 2.631 ± 0.023	3.188ThrPro: 3.188 ± 0.036	1.829ThrGln: 1.829 ± 0.022	2.394ThrArg: 2.394 ± 0.022	4.571ThrSer: 4.571 ± 0.039	3.793ThrThr: 3.793 ± 0.053	4.161ThrVal: 4.161 ± 0.035	0.561ThrTrp: 0.561 ± 0.012	1.559ThrTyr: 1.559 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	3.917ValAla: 3.917 ± 0.028	1.233ValCys: 1.233 ± 0.02	3.809ValAsp: 3.809 ± 0.028	4.197ValGlu: 4.197 ± 0.037	3.23ValPhe: 3.23 ± 0.032	3.282ValGly: 3.282 ± 0.03	1.418ValHis: 1.418 ± 0.016	4.018ValIle: 4.018 ± 0.032	3.79ValLys: 3.79 ± 0.034	5.488ValLeu: 5.488 ± 0.039	1.357ValMet: 1.357 ± 0.018	3.146ValAsn: 3.146 ± 0.025	3.329ValPro: 3.329 ± 0.031	2.42ValGln: 2.42 ± 0.022	2.809ValArg: 2.809 ± 0.024	4.634ValSer: 4.634 ± 0.032	3.532ValThr: 3.532 ± 0.031	4.456ValVal: 4.456 ± 0.039	0.613ValTrp: 0.613 ± 0.011	2.006ValTyr: 2.006 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	0.577TrpAla: 0.577 ± 0.011	0.19TrpCys: 0.19 ± 0.006	0.575TrpAsp: 0.575 ± 0.013	0.582TrpGlu: 0.582 ± 0.01	0.509TrpPhe: 0.509 ± 0.01	0.505TrpGly: 0.505 ± 0.011	0.247TrpHis: 0.247 ± 0.007	0.82TrpIle: 0.82 ± 0.015	0.808TrpLys: 0.808 ± 0.016	0.94TrpLeu: 0.94 ± 0.015	0.325TrpMet: 0.325 ± 0.008	0.61TrpAsn: 0.61 ± 0.012	0.426TrpPro: 0.426 ± 0.01	0.416TrpGln: 0.416 ± 0.009	0.611TrpArg: 0.611 ± 0.012	0.789TrpSer: 0.789 ± 0.014	0.702TrpThr: 0.702 ± 0.014	0.536TrpVal: 0.536 ± 0.01	0.145TrpTrp: 0.145 ± 0.006	0.362TrpTyr: 0.362 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.738TyrAla: 1.738 ± 0.018	0.822TyrCys: 0.822 ± 0.014	1.809TyrAsp: 1.809 ± 0.019	1.972TyrGlu: 1.972 ± 0.021	1.783TyrPhe: 1.783 ± 0.019	2.152TyrGly: 2.152 ± 0.024	0.733TyrHis: 0.733 ± 0.012	1.867TyrIle: 1.867 ± 0.024	1.731TyrLys: 1.731 ± 0.019	2.928TyrLeu: 2.928 ± 0.028	0.759TyrMet: 0.759 ± 0.012	1.634TyrAsn: 1.634 ± 0.022	1.582TyrPro: 1.582 ± 0.025	1.196TyrGln: 1.196 ± 0.017	1.775TyrArg: 1.775 ± 0.018	2.592TyrSer: 2.592 ± 0.024	1.61TyrThr: 1.61 ± 0.019	1.904TyrVal: 1.904 ± 0.02	0.419TyrTrp: 0.419 ± 0.011	1.402TyrTyr: 1.402 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.003

Statistics based on 10967 proteins (5512870 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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