Banna virus (strain Indonesia/JKT-6423/1980) (BAV)
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9YWQ1|SPIKE_BAVJK Spike protein VP9 OS=Banna virus (strain Indonesia/JKT-6423/1980) OX=649604 GN=Segment-9 PE=4 SV=1
MM1 pKa = 7.58 NNGQATITRR10 pKa = 11.84 NGGRR14 pKa = 11.84 FEE16 pKa = 3.86 IRR18 pKa = 11.84 CRR20 pKa = 11.84 HH21 pKa = 6.29 LDD23 pKa = 3.27 RR24 pKa = 11.84 DD25 pKa = 3.85 YY26 pKa = 11.97 TMPLPNATSNDD37 pKa = 3.34 NFLDD41 pKa = 4.43 CIKK44 pKa = 10.76 FITEE48 pKa = 4.25 CVGFDD53 pKa = 3.58 YY54 pKa = 11.12 VSSGFKK60 pKa = 10.73 LIANVNDD67 pKa = 3.91 FQHH70 pKa = 6.83 LNGNSTLLIGKK81 pKa = 6.81 TKK83 pKa = 10.22 IGPLILKK90 pKa = 9.61 KK91 pKa = 10.56 VRR93 pKa = 11.84 SLPCCNDD100 pKa = 2.73 ALFRR104 pKa = 11.84 NEE106 pKa = 3.65 FRR108 pKa = 11.84 ILAKK112 pKa = 9.4 MHH114 pKa = 6.73 GILRR118 pKa = 11.84 LKK120 pKa = 10.76 NDD122 pKa = 3.79 VNGHH126 pKa = 5.98 KK127 pKa = 10.52 YY128 pKa = 10.62 GIILEE133 pKa = 4.24 RR134 pKa = 11.84 CYY136 pKa = 10.65 KK137 pKa = 10.4 PKK139 pKa = 10.47 INFSNFVTAINDD151 pKa = 3.43 LDD153 pKa = 3.94 VFHH156 pKa = 7.5 SSNQHH161 pKa = 6.12 LLHH164 pKa = 7.23 GDD166 pKa = 3.77 ANPDD170 pKa = 3.48 NIMSDD175 pKa = 3.34 SEE177 pKa = 5.06 GYY179 pKa = 10.43 LKK181 pKa = 10.89 LVDD184 pKa = 4.09 PVCLLEE190 pKa = 4.24 NQVNMVNIEE199 pKa = 4.14 YY200 pKa = 10.47 EE201 pKa = 4.31 SLTQEE206 pKa = 3.92 AEE208 pKa = 3.81 KK209 pKa = 10.78 KK210 pKa = 10.59 VFINSLLQLVEE221 pKa = 4.27 KK222 pKa = 10.74 QMSATIDD229 pKa = 3.64 EE230 pKa = 4.82 IYY232 pKa = 11.44 VNLKK236 pKa = 9.35 EE237 pKa = 4.33 VNPSFNLEE245 pKa = 4.18 HH246 pKa = 6.51 GLKK249 pKa = 10.75 LSDD252 pKa = 4.19 LLDD255 pKa = 4.56 NIDD258 pKa = 4.9 VYY260 pKa = 11.67 NSDD263 pKa = 3.01 HH264 pKa = 6.62 WKK266 pKa = 10.84 LMLNHH271 pKa = 6.77 RR272 pKa = 11.84 PMMPEE277 pKa = 4.24 LSVLNDD283 pKa = 3.14 LTYY286 pKa = 11.04 YY287 pKa = 9.85 DD288 pKa = 3.74 TGEE291 pKa = 4.02 VRR293 pKa = 11.84 DD294 pKa = 4.92 LVTEE298 pKa = 4.48 DD299 pKa = 5.56 LDD301 pKa = 6.27 DD302 pKa = 4.69 EE303 pKa = 5.88 DD304 pKa = 5.88 DD305 pKa = 3.7 VV306 pKa = 4.58
Molecular weight: 34.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.906
IPC2_protein 4.94
IPC_protein 4.914
Toseland 4.851
ProMoST 5.08
Dawson 4.927
Bjellqvist 5.054
Wikipedia 4.825
Rodwell 4.825
Grimsley 4.774
Solomon 4.927
Lehninger 4.889
Nozaki 5.054
DTASelect 5.258
Thurlkill 4.863
EMBOSS 4.876
Sillero 5.105
Patrickios 3.846
IPC_peptide 4.927
IPC2_peptide 5.105
IPC2.peptide.svr19 5.037
Protein with the highest isoelectric point:
>sp|Q9INI5|VP2_BAVJK Major inner capsid protein VP2 OS=Banna virus (strain Indonesia/JKT-6423/1980) OX=649604 GN=Segment-2 PE=4 SV=1
MM1 pKa = 7.61 EE2 pKa = 5.84 LFSDD6 pKa = 3.55 SGSIVEE12 pKa = 4.36 NFKK15 pKa = 11.04 EE16 pKa = 4.34 RR17 pKa = 11.84 INKK20 pKa = 9.24 LVFDD24 pKa = 4.1 YY25 pKa = 11.16 SLNHH29 pKa = 5.68 GGFRR33 pKa = 11.84 KK34 pKa = 8.52 TYY36 pKa = 9.17 KK37 pKa = 9.19 IQRR40 pKa = 11.84 DD41 pKa = 3.27 RR42 pKa = 11.84 VYY44 pKa = 11.67 YY45 pKa = 9.25 MLGDD49 pKa = 3.5 AHH51 pKa = 6.7 HH52 pKa = 7.28 ANLSGKK58 pKa = 9.98 CLMLYY63 pKa = 10.4 NSEE66 pKa = 4.0 KK67 pKa = 10.9 DD68 pKa = 3.02 IFEE71 pKa = 4.24 GLGFKK76 pKa = 10.71 VKK78 pKa = 10.3 GSRR81 pKa = 11.84 INVSKK86 pKa = 9.03 TQILRR91 pKa = 11.84 NYY93 pKa = 8.43 EE94 pKa = 3.98 INFEE98 pKa = 4.39 TIIGVEE104 pKa = 3.99 PNGLKK109 pKa = 9.92 TISTAKK115 pKa = 10.14 DD116 pKa = 3.16 VKK118 pKa = 10.45 KK119 pKa = 10.81 LYY121 pKa = 10.41 DD122 pKa = 3.18 IYY124 pKa = 11.03 SYY126 pKa = 11.08 KK127 pKa = 10.67 SSLHH131 pKa = 6.18 PFDD134 pKa = 6.02 DD135 pKa = 5.81 FMAHH139 pKa = 7.35 CINRR143 pKa = 11.84 WGMSIPASLEE153 pKa = 3.85 RR154 pKa = 11.84 IIKK157 pKa = 10.12 SEE159 pKa = 3.96 IIKK162 pKa = 10.07 VRR164 pKa = 11.84 SGVLNRR170 pKa = 11.84 NSEE173 pKa = 4.51 LYY175 pKa = 10.73 NYY177 pKa = 9.61 IPTVDD182 pKa = 3.5 ASFSEE187 pKa = 4.79 MSRR190 pKa = 11.84 GPANVILTDD199 pKa = 3.79 GKK201 pKa = 10.65 LVPDD205 pKa = 4.64 GTCFGPILSKK215 pKa = 10.81 SVEE218 pKa = 4.06 DD219 pKa = 3.71 PRR221 pKa = 11.84 LKK223 pKa = 11.05 NEE225 pKa = 3.86 FRR227 pKa = 11.84 SKK229 pKa = 10.8 GLIMVHH235 pKa = 7.33 DD236 pKa = 4.18 YY237 pKa = 10.97 FILIGEE243 pKa = 4.55 SPGPHH248 pKa = 5.03 YY249 pKa = 10.78 KK250 pKa = 10.46 KK251 pKa = 8.89 YY252 pKa = 9.71 TKK254 pKa = 7.8 MTKK257 pKa = 10.43 DD258 pKa = 3.11 NTIFWDD264 pKa = 3.96 PRR266 pKa = 11.84 RR267 pKa = 11.84 TDD269 pKa = 3.04 HH270 pKa = 7.02 KK271 pKa = 10.45 FNNVVSYY278 pKa = 9.21 FKK280 pKa = 10.84 KK281 pKa = 9.98 EE282 pKa = 4.01 NIRR285 pKa = 11.84 DD286 pKa = 3.79 VVEE289 pKa = 4.17 YY290 pKa = 8.22 TTDD293 pKa = 3.1 ALNRR297 pKa = 11.84 GLKK300 pKa = 9.91 PLVLIDD306 pKa = 3.62 IRR308 pKa = 11.84 KK309 pKa = 9.96 DD310 pKa = 3.09 KK311 pKa = 10.7 PKK313 pKa = 10.9 NLNTPEE319 pKa = 4.16 GAIEE323 pKa = 4.15 WEE325 pKa = 4.36 RR326 pKa = 11.84 MVHH329 pKa = 7.07 DD330 pKa = 5.4 DD331 pKa = 3.76 NNLIIDD337 pKa = 4.22 MVNALDD343 pKa = 4.89 KK344 pKa = 10.97 RR345 pKa = 11.84 VTVCAKK351 pKa = 10.06 LRR353 pKa = 11.84 PAFMQVGSMRR363 pKa = 11.84 KK364 pKa = 8.67 LLRR367 pKa = 11.84 PVRR370 pKa = 11.84 ILPLPYY376 pKa = 9.98 LRR378 pKa = 11.84 RR379 pKa = 11.84 STAEE383 pKa = 3.44 FNMFVPNEE391 pKa = 3.6 ALMNGNEE398 pKa = 4.45 IYY400 pKa = 10.7 DD401 pKa = 3.58 VTYY404 pKa = 11.16 DD405 pKa = 3.53 DD406 pKa = 4.63 LVRR409 pKa = 11.84 MSSEE413 pKa = 3.93 VFVLKK418 pKa = 10.86 NIIGGLYY425 pKa = 10.64 NMYY428 pKa = 10.47 LKK430 pKa = 10.85 DD431 pKa = 3.22 MHH433 pKa = 7.11 LNLGVVNKK441 pKa = 10.26 SVSLSDD447 pKa = 3.37 GSSAIWSLSNINNEE461 pKa = 4.25 RR462 pKa = 11.84 ISNFNFNNFLYY473 pKa = 10.33 AAPYY477 pKa = 10.6 SDD479 pKa = 4.27 FATSSVKK486 pKa = 10.39 RR487 pKa = 11.84 HH488 pKa = 4.98 FKK490 pKa = 9.63 GRR492 pKa = 11.84 NYY494 pKa = 10.74 SDD496 pKa = 2.98 WCLNILDD503 pKa = 4.57 EE504 pKa = 4.76 VNLKK508 pKa = 10.88 DD509 pKa = 4.12 GVYY512 pKa = 10.25 LVPLYY517 pKa = 10.79 AIVGGGQITSHH528 pKa = 6.86 DD529 pKa = 4.03 FVNAIITDD537 pKa = 3.74 QEE539 pKa = 3.96 QLIDD543 pKa = 3.71 FTQSEE548 pKa = 4.86 RR549 pKa = 11.84 ALSTQVVKK557 pKa = 10.75 LVSFILKK564 pKa = 10.48 DD565 pKa = 3.34 SFTAKK570 pKa = 9.98 GLNWTEE576 pKa = 3.29 IDD578 pKa = 3.35 NEE580 pKa = 3.79 IRR582 pKa = 11.84 NRR584 pKa = 11.84 RR585 pKa = 11.84 LSSLSGVGFTVTKK598 pKa = 10.1 MLDD601 pKa = 3.34 GKK603 pKa = 10.59 VLVDD607 pKa = 3.66 GKK609 pKa = 10.72 VVTVSGHH616 pKa = 4.53 MLYY619 pKa = 10.1 ILLGSILGLPYY630 pKa = 10.6 GIKK633 pKa = 10.13 KK634 pKa = 9.14 YY635 pKa = 10.78 LKK637 pKa = 9.99 EE638 pKa = 3.99 IEE640 pKa = 4.44 LNILKK645 pKa = 10.07 PGSSYY650 pKa = 10.95 EE651 pKa = 3.93 RR652 pKa = 11.84 GVGGRR657 pKa = 11.84 VWHH660 pKa = 6.14 GLISHH665 pKa = 6.58 YY666 pKa = 10.79 LAVDD670 pKa = 3.59 CVIDD674 pKa = 4.55 VIDD677 pKa = 4.4 EE678 pKa = 4.4 YY679 pKa = 10.7 MVCTYY684 pKa = 10.44 EE685 pKa = 5.62 DD686 pKa = 3.62 RR687 pKa = 11.84 SKK689 pKa = 11.58 LNVVLRR695 pKa = 11.84 YY696 pKa = 9.79 VKK698 pKa = 10.64 SKK700 pKa = 10.72 LLEE703 pKa = 4.63 LGSKK707 pKa = 9.79 YY708 pKa = 10.61 DD709 pKa = 3.28 VYY711 pKa = 11.43 LSVDD715 pKa = 3.18 EE716 pKa = 4.78 RR717 pKa = 11.84 LVLL720 pKa = 4.14
Molecular weight: 82.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.911
IPC2_protein 8.083
IPC_protein 7.951
Toseland 8.317
ProMoST 8.478
Dawson 8.814
Bjellqvist 8.799
Wikipedia 8.916
Rodwell 8.916
Grimsley 8.595
Solomon 8.873
Lehninger 8.843
Nozaki 8.843
DTASelect 8.668
Thurlkill 8.756
EMBOSS 8.96
Sillero 8.96
Patrickios 4.38
IPC_peptide 8.873
IPC2_peptide 7.483
IPC2.peptide.svr19 7.629
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
5924
180
1214
493.7
55.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.313 ± 0.493
1.266 ± 0.201
6.6 ± 0.311
5.334 ± 0.208
3.764 ± 0.281
5.385 ± 0.302
2.296 ± 0.211
6.769 ± 0.266
6.617 ± 0.347
9.217 ± 0.332
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.684 ± 0.102
6.769 ± 0.267
3.309 ± 0.373
3.022 ± 0.388
4.693 ± 0.333
7.36 ± 0.363
5.807 ± 0.418
7.917 ± 0.268
0.608 ± 0.121
4.271 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here