Nocardioides terrae
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3705 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I1NHP2|A0A1I1NHP2_9ACTN Uncharacterized protein OS=Nocardioides terrae OX=574651 GN=SAMN04487968_1168 PE=4 SV=1
MM1 pKa = 7.87 SYY3 pKa = 10.86 PEE5 pKa = 4.27 DD6 pKa = 3.35 LRR8 pKa = 11.84 YY9 pKa = 7.84 TAEE12 pKa = 4.29 HH13 pKa = 5.87 EE14 pKa = 4.46 WVRR17 pKa = 11.84 TPGDD21 pKa = 3.22 HH22 pKa = 6.59 EE23 pKa = 4.7 GSVRR27 pKa = 11.84 IGITHH32 pKa = 6.7 YY33 pKa = 11.15 AQDD36 pKa = 3.69 ALGDD40 pKa = 3.66 IVYY43 pKa = 10.51 VSLPEE48 pKa = 3.85 VGATVGAGGACGEE61 pKa = 4.28 LEE63 pKa = 4.31 STKK66 pKa = 10.7 SVSDD70 pKa = 3.36 VYY72 pKa = 11.42 APVSGEE78 pKa = 3.81 VVAINDD84 pKa = 4.13 ALDD87 pKa = 3.8 ATPEE91 pKa = 4.09 LVNDD95 pKa = 4.08 DD96 pKa = 4.47 PYY98 pKa = 11.58 GGGWLFEE105 pKa = 4.65 LVPSDD110 pKa = 3.63 PAALDD115 pKa = 3.64 GLIDD119 pKa = 3.76 AAAYY123 pKa = 8.67 QATLEE128 pKa = 4.26 GG129 pKa = 3.89
Molecular weight: 13.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.79
IPC2_protein 3.783
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.872
Patrickios 0.426
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A1I1N0R9|A0A1I1N0R9_9ACTN 30S ribosomal protein S11 OS=Nocardioides terrae OX=574651 GN=rpsK PE=3 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.93 RR4 pKa = 11.84 TYY6 pKa = 10.0 QPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.16 GRR40 pKa = 11.84 KK41 pKa = 9.02 DD42 pKa = 3.21 LAVV45 pKa = 3.28
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.369
IPC2_protein 10.935
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.164
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.901
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3705
0
3705
1209282
39
2075
326.4
34.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.227 ± 0.056
0.665 ± 0.01
6.438 ± 0.033
5.518 ± 0.043
2.819 ± 0.022
9.219 ± 0.038
2.227 ± 0.015
3.559 ± 0.027
2.039 ± 0.03
10.39 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.788 ± 0.014
1.772 ± 0.021
5.543 ± 0.032
2.743 ± 0.021
7.637 ± 0.043
5.364 ± 0.027
6.092 ± 0.031
9.435 ± 0.039
1.544 ± 0.017
1.981 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here