Blueberry red ringspot virus
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q919G6|Q919G6_9VIRU Uncharacterized protein OS=Blueberry red ringspot virus OX=172220 PE=4 SV=2
MM1 pKa = 7.59 TEE3 pKa = 4.05 GNGNGKK9 pKa = 8.42 LTEE12 pKa = 4.7 KK13 pKa = 10.07 IEE15 pKa = 3.97 NMILKK20 pKa = 10.14 YY21 pKa = 10.68 EE22 pKa = 4.06 EE23 pKa = 4.25 LEE25 pKa = 4.2 KK26 pKa = 10.89 VVKK29 pKa = 10.1 QVSPEE34 pKa = 3.49 ILEE37 pKa = 3.9 IKK39 pKa = 9.76 EE40 pKa = 3.91 QVEE43 pKa = 4.24 VEE45 pKa = 3.77 NDD47 pKa = 2.79 IKK49 pKa = 11.25 DD50 pKa = 3.97 FNEE53 pKa = 3.62 SKK55 pKa = 10.86 YY56 pKa = 10.97 YY57 pKa = 11.0 NNTIRR62 pKa = 11.84 GNAQPTKK69 pKa = 10.96 EE70 pKa = 4.36 MIIDD74 pKa = 3.89 NQPEE78 pKa = 4.06 PCYY81 pKa = 9.78 QTDD84 pKa = 3.39 SPFPYY89 pKa = 9.8 LYY91 pKa = 9.25 HH92 pKa = 6.7 TFDD95 pKa = 3.75 PQLNNITDD103 pKa = 3.54 MLMYY107 pKa = 10.26 IIKK110 pKa = 10.17 NCCCKK115 pKa = 10.54 DD116 pKa = 3.05 KK117 pKa = 11.28 HH118 pKa = 5.28 EE119 pKa = 4.18 QRR121 pKa = 11.84 EE122 pKa = 4.26 KK123 pKa = 10.98 QPIIHH128 pKa = 6.56 PKK130 pKa = 9.61 ILEE133 pKa = 4.18 EE134 pKa = 4.49 KK135 pKa = 10.36 DD136 pKa = 3.5 RR137 pKa = 11.84 QIKK140 pKa = 10.23 DD141 pKa = 3.4 LEE143 pKa = 3.97 EE144 pKa = 4.98 HH145 pKa = 5.69 IRR147 pKa = 11.84 EE148 pKa = 4.37 LEE150 pKa = 3.84 EE151 pKa = 4.04 HH152 pKa = 6.3 RR153 pKa = 11.84 CITILDD159 pKa = 3.66 LKK161 pKa = 10.69 KK162 pKa = 10.95 YY163 pKa = 9.96 FGNRR167 pKa = 11.84 FNEE170 pKa = 4.3 RR171 pKa = 11.84 EE172 pKa = 4.05 DD173 pKa = 3.59 KK174 pKa = 11.23 KK175 pKa = 10.33 PIEE178 pKa = 4.54 YY179 pKa = 9.96 IEE181 pKa = 4.31 DD182 pKa = 3.53 QPRR185 pKa = 11.84 PSYY188 pKa = 10.42 RR189 pKa = 11.84 GRR191 pKa = 11.84 KK192 pKa = 8.06 PLWPQIII199 pKa = 3.8
Molecular weight: 23.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.178
IPC2_protein 5.27
IPC_protein 5.194
Toseland 5.181
ProMoST 5.334
Dawson 5.194
Bjellqvist 5.308
Wikipedia 5.041
Rodwell 5.13
Grimsley 5.105
Solomon 5.194
Lehninger 5.143
Nozaki 5.321
DTASelect 5.423
Thurlkill 5.181
EMBOSS 5.105
Sillero 5.397
Patrickios 4.075
IPC_peptide 5.207
IPC2_peptide 5.397
IPC2.peptide.svr19 5.409
Protein with the highest isoelectric point:
>tr|Q919G1|Q919G1_9VIRU Uncharacterized protein OS=Blueberry red ringspot virus OX=172220 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 10.17 PMIIMGVDD10 pKa = 3.38 VPIEE14 pKa = 3.88 RR15 pKa = 11.84 SKK17 pKa = 11.13 LKK19 pKa = 10.7 QIAKK23 pKa = 9.8 EE24 pKa = 4.08 GKK26 pKa = 8.03 LTLKK30 pKa = 10.27 SGQGIKK36 pKa = 10.29 SEE38 pKa = 4.64 GIFTIYY44 pKa = 10.19 HH45 pKa = 4.2 QEE47 pKa = 3.69 KK48 pKa = 10.72 YY49 pKa = 10.53 IIFLGKK55 pKa = 8.55 FTSEE59 pKa = 3.89 QPLEE63 pKa = 3.81 ISTAVGQEE71 pKa = 3.79 PLSLVNGKK79 pKa = 9.77 QIADD83 pKa = 5.04 RR84 pKa = 11.84 IAKK87 pKa = 8.03 MKK89 pKa = 10.77 QSDD92 pKa = 3.89 KK93 pKa = 11.47 EE94 pKa = 4.14 KK95 pKa = 9.96 IQYY98 pKa = 9.16 IHH100 pKa = 7.33 LSTIQILVKK109 pKa = 9.69 STYY112 pKa = 11.15 ASIDD116 pKa = 3.36 TPMDD120 pKa = 3.99 IIVIDD125 pKa = 3.56 NRR127 pKa = 11.84 IISKK131 pKa = 10.15 NKK133 pKa = 8.79 KK134 pKa = 7.91 EE135 pKa = 3.94 QVLGIIKK142 pKa = 10.51 GNLKK146 pKa = 10.54 YY147 pKa = 10.71 GVIKK151 pKa = 10.6 FDD153 pKa = 3.46 VSLHH157 pKa = 4.93 FAIPLVTKK165 pKa = 10.49 NLSQSIGILYY175 pKa = 9.98 KK176 pKa = 9.06 FHH178 pKa = 7.05 RR179 pKa = 11.84 QDD181 pKa = 3.46 LMEE184 pKa = 5.88 KK185 pKa = 10.25 GDD187 pKa = 3.97 YY188 pKa = 9.53 PLSITYY194 pKa = 10.19 SVGYY198 pKa = 9.91 ALSNSHH204 pKa = 6.9 PLCCYY209 pKa = 9.81 LDD211 pKa = 3.67 QEE213 pKa = 4.76 IKK215 pKa = 10.8 HH216 pKa = 6.39 IDD218 pKa = 3.73 DD219 pKa = 4.0 LFKK222 pKa = 10.37 NTSIKK227 pKa = 10.75 LVTFEE232 pKa = 5.08 KK233 pKa = 10.82 KK234 pKa = 10.32 NEE236 pKa = 3.75 NSNDD240 pKa = 2.99 IFRR243 pKa = 11.84 APPVRR248 pKa = 11.84 MIKK251 pKa = 10.06 PRR253 pKa = 11.84 EE254 pKa = 4.54 DD255 pKa = 2.94 ISKK258 pKa = 8.7 PTITDD263 pKa = 3.0 VTDD266 pKa = 3.73 PLKK269 pKa = 9.9 PTTSSFIQLAPPPNLHH285 pKa = 6.78 RR286 pKa = 11.84 KK287 pKa = 8.34 PEE289 pKa = 4.11 SMQNLEE295 pKa = 4.16 KK296 pKa = 10.58 QIQEE300 pKa = 3.87 LRR302 pKa = 11.84 RR303 pKa = 11.84 TVTNLNEE310 pKa = 4.44 KK311 pKa = 9.83 II312 pKa = 3.77
Molecular weight: 35.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.828
IPC2_protein 8.712
IPC_protein 8.58
Toseland 9.516
ProMoST 9.165
Dawson 9.736
Bjellqvist 9.37
Wikipedia 9.853
Rodwell 10.218
Grimsley 9.78
Solomon 9.75
Lehninger 9.721
Nozaki 9.545
DTASelect 9.355
Thurlkill 9.575
EMBOSS 9.926
Sillero 9.648
Patrickios 5.728
IPC_peptide 9.75
IPC2_peptide 7.79
IPC2.peptide.svr19 7.952
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2532
122
657
316.5
37.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.357 ± 0.754
1.185 ± 0.395
4.858 ± 0.4
8.728 ± 0.928
3.712 ± 0.379
3.594 ± 0.172
2.251 ± 0.239
9.716 ± 0.911
10.624 ± 0.429
8.807 ± 1.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.291 ± 0.286
6.003 ± 0.521
4.976 ± 0.321
5.213 ± 0.3
3.949 ± 0.549
6.319 ± 0.621
5.292 ± 0.373
3.397 ± 0.442
0.75 ± 0.329
4.976 ± 0.652
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here