Shewanella fodinae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3662 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2FKS3|A0A4R2FKS3_9GAMM Uncharacterized protein OS=Shewanella fodinae OX=552357 GN=EDC91_101226 PE=4 SV=1
MM1 pKa = 7.51NISSFIPSWFDD12 pKa = 3.18STPSSSSATTAAKK25 pKa = 10.91VNDD28 pKa = 4.18SVDD31 pKa = 3.45LSTGLDD37 pKa = 3.33SMFGQVIEE45 pKa = 4.19NALDD49 pKa = 3.61KK50 pKa = 11.44VSGGVTTASASTSNDD65 pKa = 3.06SLSLLGEE72 pKa = 4.17GLADD76 pKa = 3.78GLLTSFYY83 pKa = 10.37QSKK86 pKa = 10.24ISNSLTQVFNPMDD99 pKa = 4.03AAGTPSTTTQAVPSDD114 pKa = 3.95STGSAAHH121 pKa = 6.76ASDD124 pKa = 4.16SNGEE128 pKa = 4.01GLLQPFTNGQQFSFGDD144 pKa = 3.37ILDD147 pKa = 3.83VVNPLQHH154 pKa = 6.64IPLINYY160 pKa = 7.52YY161 pKa = 10.45YY162 pKa = 10.64RR163 pKa = 11.84DD164 pKa = 3.58LTGDD168 pKa = 3.49EE169 pKa = 4.48IGFVPQVVGSSIYY182 pKa = 10.68GGALGAVSSLAEE194 pKa = 4.35LGVSSLLGEE203 pKa = 4.47SPVKK207 pKa = 10.58YY208 pKa = 10.39ALNTVTGSTGSS219 pKa = 3.28

Molecular weight: 22.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2FFB7|A0A4R2FFB7_9GAMM Flagella-associated GTP-binding protein OS=Shewanella fodinae OX=552357 GN=EDC91_10824 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.2RR13 pKa = 11.84KK14 pKa = 7.57RR15 pKa = 11.84THH17 pKa = 5.9GFRR20 pKa = 11.84ARR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.93VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3662

0

3662

1185354

27

2040

323.7

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.848 ± 0.044

1.109 ± 0.017

5.404 ± 0.03

5.485 ± 0.038

3.772 ± 0.025

6.992 ± 0.038

2.308 ± 0.02

5.576 ± 0.033

4.548 ± 0.035

11.342 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.021

3.762 ± 0.029

4.243 ± 0.026

5.388 ± 0.046

5.156 ± 0.03

6.116 ± 0.032

5.156 ± 0.028

6.882 ± 0.036

1.313 ± 0.017

2.991 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski