Orenia marismortui
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3116 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R8H0E9|A0A4R8H0E9_9FIRM Anti-anti-sigma factor OS=Orenia marismortui OX=46469 GN=C7959_13516 PE=4 SV=1
MM1 pKa = 7.64 AKK3 pKa = 9.6 IQSGCKK9 pKa = 9.67 NDD11 pKa = 3.77 VEE13 pKa = 4.23 FADD16 pKa = 4.81 IIEE19 pKa = 4.69 DD20 pKa = 3.65 LSGEE24 pKa = 4.19 EE25 pKa = 3.66 VVVIVKK31 pKa = 10.48 SGGACDD37 pKa = 6.15 GVDD40 pKa = 4.26 CCCAWEE46 pKa = 4.51 GLLCSVGRR54 pKa = 11.84 DD55 pKa = 3.96 FIFLIDD61 pKa = 4.01 DD62 pKa = 4.09 GEE64 pKa = 4.77 RR65 pKa = 11.84 IFIPVDD71 pKa = 4.37 AIAAIIEE78 pKa = 4.33 DD79 pKa = 4.19 CC80 pKa = 4.84
Molecular weight: 8.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.757
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.63
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 0.769
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A4R8GSI9|A0A4R8GSI9_9FIRM Transglutaminase superfamily protein OS=Orenia marismortui OX=46469 GN=C7959_12431 PE=4 SV=1
MM1 pKa = 7.63 AGIPLNEE8 pKa = 4.51 LNNAIAYY15 pKa = 9.41 KK16 pKa = 9.92 SVPWAKK22 pKa = 10.52 DD23 pKa = 3.0 QISIKK28 pKa = 10.13 RR29 pKa = 11.84 ASFPKK34 pKa = 10.46 GKK36 pKa = 10.27 VPEE39 pKa = 4.1 HH40 pKa = 5.91 LRR42 pKa = 11.84 GYY44 pKa = 9.35 TDD46 pKa = 3.69 DD47 pKa = 3.92 LARR50 pKa = 11.84 AARR53 pKa = 11.84 ACARR57 pKa = 11.84 EE58 pKa = 4.08 TEE60 pKa = 4.25 GLRR63 pKa = 11.84 GSDD66 pKa = 3.13 RR67 pKa = 11.84 VRR69 pKa = 11.84 AMNACVSNRR78 pKa = 11.84 LGGRR82 pKa = 11.84 GRR84 pKa = 11.84 GQRR87 pKa = 11.84 QLPRR91 pKa = 11.84 VAEE94 pKa = 4.08
Molecular weight: 10.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.604
IPC_protein 10.511
Toseland 10.774
ProMoST 10.526
Dawson 10.847
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 10.979
Grimsley 10.891
Solomon 11.023
Lehninger 10.979
Nozaki 10.774
DTASelect 10.584
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.789
Patrickios 10.76
IPC_peptide 11.023
IPC2_peptide 9.75
IPC2.peptide.svr19 8.636
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3116
0
3116
939355
29
3807
301.5
34.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.991 ± 0.047
0.721 ± 0.017
6.064 ± 0.044
7.97 ± 0.048
4.054 ± 0.037
6.494 ± 0.05
1.399 ± 0.017
9.347 ± 0.048
8.528 ± 0.043
10.18 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.148 ± 0.019
5.712 ± 0.039
2.841 ± 0.024
3.01 ± 0.025
3.799 ± 0.031
6.129 ± 0.034
4.526 ± 0.038
6.453 ± 0.051
0.768 ± 0.013
3.863 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here