Cellulomonas cellasea DSM 20118

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; Cellulomonas cellasea

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3560 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0BBD4|A0A0A0BBD4_9CELL RNA polymerase subunit sigma-24 OS=Cellulomonas cellasea DSM 20118 OX=1408250 GN=Q760_12500 PE=3 SV=1
MM1 pKa = 7.21NGMRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84GTAVVAAGAGLTLLLAACSGGGDD31 pKa = 3.52EE32 pKa = 5.58GGGDD36 pKa = 3.87AEE38 pKa = 4.55GTANEE43 pKa = 4.49TDD45 pKa = 3.08CSAYY49 pKa = 10.11EE50 pKa = 4.07EE51 pKa = 4.46YY52 pKa = 11.32GDD54 pKa = 4.89LSGKK58 pKa = 7.18TVSVYY63 pKa = 10.32TSIVDD68 pKa = 3.71PEE70 pKa = 4.27SEE72 pKa = 4.15EE73 pKa = 4.44QINSYY78 pKa = 10.53QPFVDD83 pKa = 3.94CTGVEE88 pKa = 3.61IDD90 pKa = 3.94YY91 pKa = 10.77EE92 pKa = 4.55GSRR95 pKa = 11.84EE96 pKa = 3.99FEE98 pKa = 3.84AQLPVRR104 pKa = 11.84LTAGNPPDD112 pKa = 3.5IAYY115 pKa = 9.99VPQPGFLKK123 pKa = 10.84SLVADD128 pKa = 5.0FPDD131 pKa = 3.46QVVPAPQSVIDD142 pKa = 4.12NATEE146 pKa = 4.86FYY148 pKa = 10.33TEE150 pKa = 3.49EE151 pKa = 3.86WVNYY155 pKa = 7.14GTVDD159 pKa = 3.13GTLYY163 pKa = 9.35ATPLGANVKK172 pKa = 10.15SFVWYY177 pKa = 10.41SPLAFEE183 pKa = 4.87DD184 pKa = 3.29AGYY187 pKa = 9.64EE188 pKa = 4.69IPTTWDD194 pKa = 2.95EE195 pKa = 5.71LMDD198 pKa = 4.45LSQQIVDD205 pKa = 4.07DD206 pKa = 5.79GGVPWCAGIEE216 pKa = 4.19SGDD219 pKa = 3.58ATGWPATDD227 pKa = 3.11WLEE230 pKa = 4.41DD231 pKa = 3.59VVLRR235 pKa = 11.84TAGADD240 pKa = 4.48VYY242 pKa = 10.24DD243 pKa = 3.09QWVNHH248 pKa = 6.96EE249 pKa = 4.64IPFNDD254 pKa = 3.74PQIVEE259 pKa = 4.14ALGTVGEE266 pKa = 4.09ILKK269 pKa = 10.38NDD271 pKa = 3.57EE272 pKa = 4.36YY273 pKa = 11.96VNGGLGNVQSIATAAWNEE291 pKa = 4.22SANGIPDD298 pKa = 4.05GTCWLHH304 pKa = 6.31RR305 pKa = 11.84AANFYY310 pKa = 8.96QANWDD315 pKa = 3.54EE316 pKa = 4.33SLEE319 pKa = 4.12VAEE322 pKa = 5.63DD323 pKa = 3.81GDD325 pKa = 4.2VYY327 pKa = 11.44AFYY330 pKa = 11.05LPGVSEE336 pKa = 4.61DD337 pKa = 4.24DD338 pKa = 4.18KK339 pKa = 11.21PLLGAGEE346 pKa = 4.4FVTAFSDD353 pKa = 3.8RR354 pKa = 11.84PEE356 pKa = 4.07VEE358 pKa = 3.94AFQAFLSSPEE368 pKa = 3.89WANSKK373 pKa = 10.93ASVTAQGWFSANNGLDD389 pKa = 3.4PEE391 pKa = 5.26LIKK394 pKa = 11.18SPIDD398 pKa = 3.26KK399 pKa = 10.59LAFEE403 pKa = 5.43LLTSDD408 pKa = 4.22EE409 pKa = 4.3YY410 pKa = 11.16TFRR413 pKa = 11.84FDD415 pKa = 6.12GSDD418 pKa = 3.49QMPGAVGTGSFWTEE432 pKa = 3.25MTAWVANDD440 pKa = 3.71KK441 pKa = 11.03SDD443 pKa = 3.9EE444 pKa = 4.26DD445 pKa = 3.72VLSAIEE451 pKa = 4.2ASWPTSS457 pKa = 3.11

Molecular weight: 49.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0B6P3|A0A0A0B6P3_9CELL Membrane protein OS=Cellulomonas cellasea DSM 20118 OX=1408250 GN=Q760_12970 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3560

0

3560

1137429

29

2089

319.5

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.435 ± 0.061

0.57 ± 0.009

6.403 ± 0.036

5.371 ± 0.039

2.492 ± 0.022

9.593 ± 0.039

2.161 ± 0.023

2.682 ± 0.029

1.3 ± 0.026

10.369 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.464 ± 0.013

1.505 ± 0.025

6.042 ± 0.033

2.558 ± 0.022

7.917 ± 0.046

4.949 ± 0.029

6.576 ± 0.041

10.193 ± 0.049

1.577 ± 0.019

1.844 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski