Durania virus
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F4ZCL0|F4ZCL0_9VIRU Nucleoprotein OS=Durania virus OX=1006585 GN=NP PE=3 SV=1
MM1 pKa = 7.42 TSYY4 pKa = 11.29 LFDD7 pKa = 3.42 TPVIVRR13 pKa = 11.84 PNGYY17 pKa = 9.24 FDD19 pKa = 4.06 RR20 pKa = 11.84 EE21 pKa = 4.37 VYY23 pKa = 7.9 VTYY26 pKa = 9.66 MAHH29 pKa = 6.72 NKK31 pKa = 9.15 YY32 pKa = 10.21 CEE34 pKa = 4.27 HH35 pKa = 6.27 TVSVHH40 pKa = 5.22 NCMEE44 pKa = 3.96 IPVRR48 pKa = 11.84 NFGVSMLHH56 pKa = 6.51 KK57 pKa = 10.34 EE58 pKa = 4.3 SFSDD62 pKa = 4.96 FYY64 pKa = 11.18 ILGLFPFRR72 pKa = 11.84 WGDD75 pKa = 3.05 KK76 pKa = 9.13 MGNSRR81 pKa = 11.84 VTRR84 pKa = 11.84 EE85 pKa = 3.97 SFPFMKK91 pKa = 10.31 EE92 pKa = 3.61 LLADD96 pKa = 3.34 ICKK99 pKa = 8.72 MHH101 pKa = 7.25 DD102 pKa = 3.37 SEE104 pKa = 5.08 FFRR107 pKa = 11.84 SFLPNIKK114 pKa = 9.94 KK115 pKa = 10.41 ALSWPLGYY123 pKa = 9.73 PSLDD127 pKa = 4.15 FIRR130 pKa = 11.84 MTDD133 pKa = 3.65 PDD135 pKa = 3.22 NSMPHH140 pKa = 5.74 FRR142 pKa = 11.84 EE143 pKa = 3.55 ISNNATLIMQMGQPAIDD160 pKa = 4.12 LDD162 pKa = 3.78 QGFVNAHH169 pKa = 5.65 KK170 pKa = 10.11 RR171 pKa = 11.84 IVMEE175 pKa = 4.48 SITRR179 pKa = 11.84 GFGHH183 pKa = 7.36 KK184 pKa = 10.29 DD185 pKa = 3.34 FPGKK189 pKa = 10.25 NLIFEE194 pKa = 4.48 VASLQCVRR202 pKa = 11.84 MLNAVPADD210 pKa = 3.79 LLYY213 pKa = 11.03 CQITNSLTSTLMKK226 pKa = 10.25 HH227 pKa = 4.81 RR228 pKa = 11.84 AEE230 pKa = 4.54 MEE232 pKa = 4.14 SHH234 pKa = 6.04 PQGPLGNQKK243 pKa = 9.93 WIPDD247 pKa = 3.95 PDD249 pKa = 3.33 SWLLDD254 pKa = 3.87 DD255 pKa = 5.16 QSSTLHH261 pKa = 6.5 EE262 pKa = 4.5 EE263 pKa = 3.93 FNLIISDD270 pKa = 4.83 SEE272 pKa = 4.73 DD273 pKa = 3.27 EE274 pKa = 4.93 DD275 pKa = 3.93 EE276 pKa = 4.77
Molecular weight: 31.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.166
IPC2_protein 5.156
IPC_protein 5.143
Toseland 5.27
ProMoST 5.27
Dawson 5.245
Bjellqvist 5.347
Wikipedia 5.169
Rodwell 5.169
Grimsley 5.283
Solomon 5.245
Lehninger 5.207
Nozaki 5.41
DTASelect 5.588
Thurlkill 5.347
EMBOSS 5.321
Sillero 5.486
Patrickios 3.91
IPC_peptide 5.245
IPC2_peptide 5.499
IPC2.peptide.svr19 5.339
Protein with the highest isoelectric point:
>tr|F4ZCL1|F4ZCL1_9VIRU Glycoprotein OS=Durania virus OX=1006585 GN=GP PE=4 SV=1
MM1 pKa = 7.94 ADD3 pKa = 3.97 FEE5 pKa = 4.33 RR6 pKa = 11.84 LAIEE10 pKa = 4.29 FSEE13 pKa = 4.5 AGVNIADD20 pKa = 3.43 VVAWVNEE27 pKa = 3.79 FAYY30 pKa = 10.49 QGFDD34 pKa = 2.91 AKK36 pKa = 10.46 RR37 pKa = 11.84 VIEE40 pKa = 4.12 LLQQRR45 pKa = 11.84 GGTSWQEE52 pKa = 4.17 DD53 pKa = 3.64 ARR55 pKa = 11.84 KK56 pKa = 9.14 MIVLALTRR64 pKa = 11.84 GNKK67 pKa = 7.82 IEE69 pKa = 4.12 KK70 pKa = 9.33 MIMKK74 pKa = 9.73 MSEE77 pKa = 3.91 EE78 pKa = 4.12 GKK80 pKa = 8.59 KK81 pKa = 8.63 TVLSLKK87 pKa = 10.13 KK88 pKa = 10.14 RR89 pKa = 11.84 YY90 pKa = 7.5 QLKK93 pKa = 10.3 SGNPGRR99 pKa = 11.84 DD100 pKa = 3.45 DD101 pKa = 3.54 LTLSRR106 pKa = 11.84 VAAALAGWTCQALPHH121 pKa = 6.37 VEE123 pKa = 3.94 NFLPVTGEE131 pKa = 3.97 AMDD134 pKa = 4.46 ALSPSFPRR142 pKa = 11.84 CMMHH146 pKa = 6.97 PSFAGLVDD154 pKa = 3.48 TTLPPEE160 pKa = 4.23 TQDD163 pKa = 4.51 AILAAHH169 pKa = 6.45 SLFLVQFSKK178 pKa = 11.16 VINPGLRR185 pKa = 11.84 GKK187 pKa = 10.37 AKK189 pKa = 10.37 SEE191 pKa = 3.98 VVASFKK197 pKa = 10.94 QPMLAAVNGGFIGPEE212 pKa = 3.92 KK213 pKa = 9.54 RR214 pKa = 11.84 RR215 pKa = 11.84 KK216 pKa = 8.28 MLQSLGVVDD225 pKa = 4.19 VNGIPSEE232 pKa = 4.11 AVKK235 pKa = 10.75 VAAQKK240 pKa = 10.28 FRR242 pKa = 11.84 ASQQ245 pKa = 2.95
Molecular weight: 26.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.657
IPC2_protein 8.565
IPC_protein 8.507
Toseland 9.575
ProMoST 9.092
Dawson 9.706
Bjellqvist 9.341
Wikipedia 9.78
Rodwell 10.292
Grimsley 9.721
Solomon 9.765
Lehninger 9.765
Nozaki 9.648
DTASelect 9.297
Thurlkill 9.589
EMBOSS 9.955
Sillero 9.648
Patrickios 10.028
IPC_peptide 9.78
IPC2_peptide 7.819
IPC2.peptide.svr19 7.595
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3926
245
2085
981.5
110.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.323 ± 1.088
2.878 ± 0.852
5.96 ± 0.487
6.623 ± 0.378
4.712 ± 0.366
5.935 ± 0.276
2.598 ± 0.291
6.47 ± 0.528
6.393 ± 0.346
8.839 ± 0.178
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.184 ± 0.64
3.49 ± 0.341
3.948 ± 0.424
3.082 ± 0.32
5.527 ± 0.214
9.883 ± 0.726
4.992 ± 0.275
6.342 ± 0.411
1.248 ± 0.124
2.573 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here