Baminivirus
Average proteome isoelectric point is 8.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J9TCS3|J9TCS3_9GEMI Putative movement protein OS=Baminivirus OX=1229324 PE=4 SV=1
MM1 pKa = 7.1 AQPRR5 pKa = 11.84 FRR7 pKa = 11.84 LQGKK11 pKa = 9.6 NILLTYY17 pKa = 6.94 PQCNLEE23 pKa = 4.26 VKK25 pKa = 10.03 HH26 pKa = 6.17 VCDD29 pKa = 4.01 EE30 pKa = 4.19 LALRR34 pKa = 11.84 LPPGRR39 pKa = 11.84 CSGVLEE45 pKa = 4.09 RR46 pKa = 11.84 HH47 pKa = 5.56 QDD49 pKa = 3.74 GNPHH53 pKa = 6.88 LHH55 pKa = 6.51 ILWEE59 pKa = 3.94 LRR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 LDD65 pKa = 3.34 TQNPRR70 pKa = 11.84 FFDD73 pKa = 3.03 VDD75 pKa = 3.67 TFHH78 pKa = 7.26 PNVVVVRR85 pKa = 11.84 SVRR88 pKa = 11.84 GARR91 pKa = 11.84 DD92 pKa = 3.53 YY93 pKa = 10.76 IAKK96 pKa = 9.2 TGAEE100 pKa = 4.45 VYY102 pKa = 9.72 TNEE105 pKa = 4.42 PGEE108 pKa = 4.36 PEE110 pKa = 4.16 CEE112 pKa = 3.86 SWGEE116 pKa = 3.86 ILEE119 pKa = 4.35 QATSRR124 pKa = 11.84 EE125 pKa = 3.98 GFLDD129 pKa = 3.81 LVRR132 pKa = 11.84 KK133 pKa = 9.53 HH134 pKa = 6.14 KK135 pKa = 10.57 PRR137 pKa = 11.84 DD138 pKa = 3.62 FILNMEE144 pKa = 4.36 RR145 pKa = 11.84 LEE147 pKa = 4.1 YY148 pKa = 10.33 AANILFAEE156 pKa = 4.82 PEE158 pKa = 3.91 QSFVSMYY165 pKa = 8.02 TTFAPPSACHH175 pKa = 6.63 DD176 pKa = 2.68 WWLQYY181 pKa = 9.97 GARR184 pKa = 11.84 PRR186 pKa = 11.84 LEE188 pKa = 4.34 GRR190 pKa = 11.84 LQSLYY195 pKa = 11.06 VEE197 pKa = 4.66 GASRR201 pKa = 11.84 IGKK204 pKa = 6.82 TEE206 pKa = 3.79 WARR209 pKa = 11.84 SLGPHH214 pKa = 6.12 VYY216 pKa = 10.12 FNNMYY221 pKa = 10.78 NLDD224 pKa = 4.08 DD225 pKa = 4.66 LKK227 pKa = 11.3 SALRR231 pKa = 11.84 YY232 pKa = 10.36 AEE234 pKa = 3.99 FAVFDD239 pKa = 5.57 DD240 pKa = 4.26 IPFDD244 pKa = 5.0 RR245 pKa = 11.84 LPAWKK250 pKa = 10.39 SFFGCQHH257 pKa = 6.68 TFTLTDD263 pKa = 3.87 RR264 pKa = 11.84 YY265 pKa = 9.97 RR266 pKa = 11.84 KK267 pKa = 10.13 KK268 pKa = 10.13 FTISDD273 pKa = 3.41 WSKK276 pKa = 10.72 PSIFLVNPDD285 pKa = 3.31 MTYY288 pKa = 10.96 DD289 pKa = 2.99 AWNKK293 pKa = 8.31 GRR295 pKa = 11.84 EE296 pKa = 3.97 WEE298 pKa = 4.3 YY299 pKa = 10.47 IKK301 pKa = 11.02 QNAVIVKK308 pKa = 8.48 TDD310 pKa = 2.82 SRR312 pKa = 11.84 FYY314 pKa = 11.47
Molecular weight: 36.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.541
IPC2_protein 6.561
IPC_protein 6.722
Toseland 6.737
ProMoST 7.263
Dawson 7.205
Bjellqvist 7.117
Wikipedia 7.176
Rodwell 7.19
Grimsley 6.854
Solomon 7.249
Lehninger 7.249
Nozaki 7.439
DTASelect 7.497
Thurlkill 7.527
EMBOSS 7.556
Sillero 7.614
Patrickios 4.24
IPC_peptide 7.249
IPC2_peptide 7.146
IPC2.peptide.svr19 6.988
Protein with the highest isoelectric point:
>tr|J9TCS3|J9TCS3_9GEMI Putative movement protein OS=Baminivirus OX=1229324 PE=4 SV=1
MM1 pKa = 7.98 RR2 pKa = 11.84 DD3 pKa = 3.21 LRR5 pKa = 11.84 DD6 pKa = 3.87 FYY8 pKa = 11.59 VPRR11 pKa = 11.84 LRR13 pKa = 11.84 MNEE16 pKa = 4.52 FIRR19 pKa = 11.84 RR20 pKa = 11.84 PIGDD24 pKa = 4.08 GLKK27 pKa = 10.19 EE28 pKa = 4.21 SPDD31 pKa = 3.6 LCVLPGYY38 pKa = 10.26 LGRR41 pKa = 11.84 TNQVRR46 pKa = 11.84 LSYY49 pKa = 10.08 PGPTFTRR56 pKa = 11.84 SVTRR60 pKa = 11.84 SCPARR65 pKa = 11.84 SRR67 pKa = 11.84 DD68 pKa = 3.35 PHH70 pKa = 7.97 VIFLGWFGGAGGFTPQHH87 pKa = 6.19 FLSIAA92 pKa = 3.25
Molecular weight: 10.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.604
IPC_protein 10.701
Toseland 10.248
ProMoST 10.277
Dawson 10.496
Bjellqvist 10.365
Wikipedia 10.818
Rodwell 10.409
Grimsley 10.613
Solomon 10.643
Lehninger 10.584
Nozaki 10.335
DTASelect 10.35
Thurlkill 10.35
EMBOSS 10.701
Sillero 10.452
Patrickios 10.087
IPC_peptide 10.643
IPC2_peptide 9.794
IPC2.peptide.svr19 8.279
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
897
92
491
299.0
34.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.581 ± 1.249
1.226 ± 0.453
5.797 ± 0.116
4.125 ± 1.312
4.794 ± 1.002
7.246 ± 1.014
3.456 ± 0.464
4.125 ± 0.138
5.463 ± 1.182
8.361 ± 0.61
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.23 ± 0.32
3.79 ± 0.357
4.905 ± 1.327
3.567 ± 0.297
8.027 ± 1.164
6.466 ± 0.672
6.912 ± 1.209
5.351 ± 0.196
2.118 ± 0.342
4.459 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here