Torque teno virus 20
Average proteome isoelectric point is 8.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q91CZ8|Q91CZ8_9VIRU ORF4 ORF3 ORF2 ORF1 genes clone: SAa-10 OS=Torque teno virus 20 OX=687359 PE=4 SV=1
MM1 pKa = 7.12 SLWRR5 pKa = 11.84 PPMRR9 pKa = 11.84 HH10 pKa = 4.53 VPSRR14 pKa = 11.84 EE15 pKa = 3.83 RR16 pKa = 11.84 DD17 pKa = 3.2 WVEE20 pKa = 3.36 AAFRR24 pKa = 11.84 AHH26 pKa = 7.29 AAFCGCTNPVIHH38 pKa = 6.14 FTSVLARR45 pKa = 11.84 FNLQGGPQTPPDD57 pKa = 4.95 DD58 pKa = 4.61 PPEE61 pKa = 4.32 GAPVLGALPAPSPRR75 pKa = 11.84 RR76 pKa = 11.84 HH77 pKa = 5.46 TRR79 pKa = 11.84 SEE81 pKa = 4.14 NPGPQQWPTGGAAAAGRR98 pKa = 11.84 GDD100 pKa = 4.01 GDD102 pKa = 3.96 GGAADD107 pKa = 4.05 AADD110 pKa = 4.2 EE111 pKa = 4.14 YY112 pKa = 10.84 RR113 pKa = 11.84 AEE115 pKa = 5.56 DD116 pKa = 3.88 IDD118 pKa = 4.86 DD119 pKa = 4.1 LFAAIEE125 pKa = 4.31 RR126 pKa = 11.84 DD127 pKa = 3.81 TQQ129 pKa = 3.29
Molecular weight: 13.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.008
IPC2_protein 5.016
IPC_protein 4.902
Toseland 4.838
ProMoST 5.067
Dawson 4.927
Bjellqvist 5.054
Wikipedia 4.838
Rodwell 4.825
Grimsley 4.774
Solomon 4.927
Lehninger 4.876
Nozaki 5.054
DTASelect 5.258
Thurlkill 4.863
EMBOSS 4.876
Sillero 5.105
Patrickios 4.24
IPC_peptide 4.927
IPC2_peptide 5.105
IPC2.peptide.svr19 5.051
Protein with the highest isoelectric point:
>tr|Q91CZ9|Q91CZ9_9VIRU ORF4 ORF3 ORF2 ORF1 genes clone: SAa-10 OS=Torque teno virus 20 OX=687359 PE=4 SV=1
MM1 pKa = 7.12 SLWRR5 pKa = 11.84 PPMRR9 pKa = 11.84 HH10 pKa = 4.53 VPSRR14 pKa = 11.84 EE15 pKa = 3.83 RR16 pKa = 11.84 DD17 pKa = 3.2 WVEE20 pKa = 3.36 AAFRR24 pKa = 11.84 AHH26 pKa = 7.29 AAFCGCTNPVIHH38 pKa = 6.14 FTSVLARR45 pKa = 11.84 FNLQGGPQTPPDD57 pKa = 4.95 DD58 pKa = 4.61 PPEE61 pKa = 4.32 GAPVLGALPAPSPRR75 pKa = 11.84 RR76 pKa = 11.84 HH77 pKa = 5.46 TRR79 pKa = 11.84 SEE81 pKa = 4.14 NPGPQQWPTGGAAAAGRR98 pKa = 11.84 GDD100 pKa = 4.01 GDD102 pKa = 3.96 GGAADD107 pKa = 4.05 AADD110 pKa = 4.2 EE111 pKa = 4.14 YY112 pKa = 10.84 RR113 pKa = 11.84 AEE115 pKa = 5.56 DD116 pKa = 3.88 IDD118 pKa = 4.86 DD119 pKa = 4.1 LFAAIEE125 pKa = 4.26 RR126 pKa = 11.84 DD127 pKa = 3.55 TQSRR131 pKa = 11.84 RR132 pKa = 11.84 RR133 pKa = 11.84 GKK135 pKa = 10.32 ARR137 pKa = 11.84 RR138 pKa = 11.84 KK139 pKa = 8.6 LHH141 pKa = 4.91 FRR143 pKa = 11.84 GRR145 pKa = 11.84 KK146 pKa = 8.41 HH147 pKa = 6.55 PYY149 pKa = 8.63 LARR152 pKa = 11.84 RR153 pKa = 11.84 DD154 pKa = 3.84 ADD156 pKa = 3.67 TTTQSPAPKK165 pKa = 9.75 AAPRR169 pKa = 11.84 PARR172 pKa = 11.84 ATSPAQAPLPPSPQNPGGGPPQSPTIHH199 pKa = 6.41 PAVNRR204 pKa = 11.84 VYY206 pKa = 10.97 LFPEE210 pKa = 4.82 RR211 pKa = 11.84 EE212 pKa = 4.29 AKK214 pKa = 9.34 PAPTRR219 pKa = 11.84 GDD221 pKa = 3.28 WEE223 pKa = 4.59 TEE225 pKa = 3.82 MQACKK230 pKa = 10.62 AFDD233 pKa = 3.27 RR234 pKa = 11.84 PMRR237 pKa = 11.84 SNLSNSPFYY246 pKa = 10.59 PWMPSYY252 pKa = 10.37 RR253 pKa = 11.84 VSFRR257 pKa = 11.84 LGFKK261 pKa = 10.05 PP262 pKa = 4.3
Molecular weight: 28.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.311
IPC_protein 9.94
Toseland 10.204
ProMoST 9.97
Dawson 10.379
Bjellqvist 10.116
Wikipedia 10.599
Rodwell 10.496
Grimsley 10.452
Solomon 10.467
Lehninger 10.423
Nozaki 10.248
DTASelect 10.101
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.321
Patrickios 10.131
IPC_peptide 10.467
IPC2_peptide 9.165
IPC2.peptide.svr19 8.462
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1435
129
750
358.8
40.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.526 ± 2.545
1.533 ± 0.146
5.993 ± 0.272
4.042 ± 0.477
4.181 ± 0.689
6.481 ± 0.905
2.439 ± 0.187
3.624 ± 0.814
5.157 ± 1.004
6.202 ± 0.675
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.812 ± 0.254
3.345 ± 0.615
9.338 ± 1.868
4.321 ± 0.165
10.105 ± 0.335
7.038 ± 1.65
6.132 ± 0.463
4.46 ± 0.775
2.23 ± 0.321
4.042 ± 1.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here