Schistosoma curassoni
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22688 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A183K9Y4|A0A183K9Y4_9TREM Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS11817 PE=4 SV=1
MM1 pKa = 7.28 NLSLPQQFWTKK12 pKa = 10.73 LFIVLNSCFVIFGIVLLALGIKK34 pKa = 10.24 GLEE37 pKa = 4.1 TLNKK41 pKa = 9.96 FGTILTGIIPPIIPIAIFIGCLILIGTTIGYY72 pKa = 9.54 IGLWKK77 pKa = 9.62 PKK79 pKa = 10.16 KK80 pKa = 9.69 FIHH83 pKa = 6.45 IACLCLAVIIEE94 pKa = 4.2 IAIATATATMGEE106 pKa = 4.31 KK107 pKa = 10.31 FQTAANHH114 pKa = 6.08 SVLQAVKK121 pKa = 10.14 QFYY124 pKa = 10.54 SNPYY128 pKa = 8.77 CQVEE132 pKa = 4.01 MDD134 pKa = 3.59 KK135 pKa = 11.12 LQRR138 pKa = 11.84 NYY140 pKa = 9.79 RR141 pKa = 11.84 CCGATSYY148 pKa = 11.45 LDD150 pKa = 3.52 YY151 pKa = 11.22 VQTNKK156 pKa = 9.09 TVPFSCFIGTLVYY169 pKa = 10.5 ARR171 pKa = 11.84 HH172 pKa = 6.35 IFDD175 pKa = 6.13 DD176 pKa = 5.63 DD177 pKa = 4.7 DD178 pKa = 6.19 ALDD181 pKa = 5.78 DD182 pKa = 6.58 DD183 pKa = 5.27 DD184 pKa = 5.59 TLDD187 pKa = 5.99 DD188 pKa = 5.79 DD189 pKa = 6.15 ALDD192 pKa = 5.71 DD193 pKa = 5.85 DD194 pKa = 6.35 DD195 pKa = 7.56 DD196 pKa = 5.01 DD197 pKa = 6.59 ALDD200 pKa = 4.67 EE201 pKa = 5.63 DD202 pKa = 5.99 DD203 pKa = 6.66 DD204 pKa = 5.69 DD205 pKa = 6.59 ALDD208 pKa = 5.87 DD209 pKa = 5.74 DD210 pKa = 6.41 DD211 pKa = 7.57 DD212 pKa = 5.58 DD213 pKa = 6.57 ALDD216 pKa = 5.81 DD217 pKa = 5.74 DD218 pKa = 6.41 DD219 pKa = 7.57 DD220 pKa = 5.58 DD221 pKa = 6.57 ALDD224 pKa = 5.81 DD225 pKa = 5.74 DD226 pKa = 6.41 DD227 pKa = 7.57 DD228 pKa = 5.58 DD229 pKa = 6.57 ALDD232 pKa = 6.02 DD233 pKa = 6.43 DD234 pKa = 6.52 DD235 pKa = 7.72 DD236 pKa = 7.25 DD237 pKa = 7.47 DD238 pKa = 7.49 DD239 pKa = 7.17 DD240 pKa = 6.74 GDD242 pKa = 4.16 YY243 pKa = 11.47 DD244 pKa = 5.31 ALNDD248 pKa = 5.4 DD249 pKa = 5.43 DD250 pKa = 6.44 ALDD253 pKa = 5.67 DD254 pKa = 5.71 DD255 pKa = 6.41 DD256 pKa = 7.57 DD257 pKa = 5.58 DD258 pKa = 6.57 ALDD261 pKa = 5.2 DD262 pKa = 5.86 DD263 pKa = 6.31 DD264 pKa = 7.37 DD265 pKa = 3.75 ITDD268 pKa = 4.84 FIRR271 pKa = 11.84 KK272 pKa = 8.12 FWQKK276 pKa = 10.6 IFITLNTLFVIFNIILLILGIITQNTLSKK305 pKa = 10.04 YY306 pKa = 5.68 TTILNTPISAIIPTILFTGCFGFIAALIGYY336 pKa = 8.88 IGLWKK341 pKa = 10.17 PMNLIALMHH350 pKa = 6.95 IISLCIVTLIEE361 pKa = 3.98 IGIATTSAVMYY372 pKa = 9.7 DD373 pKa = 3.19 QFYY376 pKa = 7.35 TTTHH380 pKa = 6.06 SALLDD385 pKa = 3.71 SVKK388 pKa = 10.29 FYY390 pKa = 11.55 YY391 pKa = 10.48 EE392 pKa = 3.57 NSQYY396 pKa = 10.82 EE397 pKa = 4.2 MEE399 pKa = 4.43 MDD401 pKa = 3.59 HH402 pKa = 6.69 LQTEE406 pKa = 5.42 FKK408 pKa = 10.84 CCGAEE413 pKa = 4.02 SYY415 pKa = 11.32 LDD417 pKa = 3.69 YY418 pKa = 11.04 RR419 pKa = 11.84 KK420 pKa = 10.52 LGMNIPFSCIIGYY433 pKa = 9.13 LVYY436 pKa = 10.88 ARR438 pKa = 11.84 VPLEE442 pKa = 4.04 EE443 pKa = 4.59 PFHH446 pKa = 6.13 GVSNRR451 pKa = 11.84 KK452 pKa = 9.63 VITALIPLTAFKK464 pKa = 9.78 ITDD467 pKa = 3.52 SEE469 pKa = 4.32 FFYY472 pKa = 11.35 SDD474 pKa = 4.84 LEE476 pKa = 5.17 DD477 pKa = 4.05 IMINKK482 pKa = 8.97
Molecular weight: 54.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.656
IPC_protein 3.732
Toseland 3.478
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.541
Grimsley 3.389
Solomon 3.745
Lehninger 3.706
Nozaki 3.846
DTASelect 4.19
Thurlkill 3.541
EMBOSS 3.719
Sillero 3.859
Patrickios 0.846
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A183JGX8|A0A183JGX8_9TREM Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS1952 PE=4 SV=1
MM1 pKa = 7.33 MLTTKK6 pKa = 10.19 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 IRR11 pKa = 11.84 TVKK14 pKa = 9.14 RR15 pKa = 11.84 TNPINAFQKK24 pKa = 9.18 MRR26 pKa = 11.84 TIINIII32 pKa = 3.49
Molecular weight: 3.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.443
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.09
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22688
0
22688
5552202
29
4267
244.7
27.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.506 ± 0.018
2.092 ± 0.012
5.36 ± 0.046
5.507 ± 0.021
3.799 ± 0.014
4.39 ± 0.024
2.921 ± 0.012
6.691 ± 0.02
5.769 ± 0.021
9.558 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.166 ± 0.015
7.165 ± 0.028
4.457 ± 0.017
4.326 ± 0.016
4.947 ± 0.017
9.985 ± 0.032
6.635 ± 0.038
5.359 ± 0.025
1.021 ± 0.006
3.23 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here