Klebsiella phage vB_KpnP_IME335

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; unclassified Przondovirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6CUM1|A0A5J6CUM1_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnP_IME335 OX=2601649 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.1YY9 pKa = 10.68DD10 pKa = 4.19LLAATVEE17 pKa = 4.25LFNEE21 pKa = 5.31RR22 pKa = 11.84IQCDD26 pKa = 3.98EE27 pKa = 4.25LTEE30 pKa = 5.1HH31 pKa = 7.6DD32 pKa = 4.36DD33 pKa = 3.37WSDD36 pKa = 3.34ALHH39 pKa = 6.13EE40 pKa = 4.49VVDD43 pKa = 4.59GQVPHH48 pKa = 6.81YY49 pKa = 8.66YY50 pKa = 10.32HH51 pKa = 7.62EE52 pKa = 4.84IFTVMASDD60 pKa = 5.46GIDD63 pKa = 3.43HH64 pKa = 6.8EE65 pKa = 5.07FDD67 pKa = 4.04DD68 pKa = 5.49SGLIPDD74 pKa = 4.27TKK76 pKa = 10.52DD77 pKa = 3.0VSRR80 pKa = 11.84ICQARR85 pKa = 11.84IYY87 pKa = 9.16EE88 pKa = 4.07ALYY91 pKa = 11.03NDD93 pKa = 3.92VLNDD97 pKa = 3.6SGIVWWEE104 pKa = 3.73EE105 pKa = 3.66PEE107 pKa = 4.01EE108 pKa = 5.03DD109 pKa = 5.56DD110 pKa = 6.6EE111 pKa = 5.17EE112 pKa = 6.13DD113 pKa = 4.9CDD115 pKa = 4.89DD116 pKa = 3.87

Molecular weight: 13.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6CUI7|A0A5J6CUI7_9CAUD Tail tubular protein A OS=Klebsiella phage vB_KpnP_IME335 OX=2601649 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84LHH4 pKa = 6.7FNKK7 pKa = 10.46SNGIFSVRR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 4.04DD18 pKa = 3.6RR19 pKa = 11.84STVAATEE26 pKa = 3.98RR27 pKa = 11.84HH28 pKa = 5.03GKK30 pKa = 8.71IPRR33 pKa = 11.84IGDD36 pKa = 3.66TFEE39 pKa = 4.6LAPSVHH45 pKa = 6.56ILVTRR50 pKa = 11.84GLYY53 pKa = 9.93EE54 pKa = 4.12LAQTKK59 pKa = 9.86SRR61 pKa = 11.84PFVPVVVTKK70 pKa = 9.98WPRR73 pKa = 11.84LRR75 pKa = 11.84LFWEE79 pKa = 4.85RR80 pKa = 11.84IKK82 pKa = 11.06EE83 pKa = 4.25VVNDD87 pKa = 3.83

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12020

36

1321

267.1

29.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.185 ± 0.523

0.94 ± 0.155

6.531 ± 0.214

6.947 ± 0.484

3.594 ± 0.189

7.937 ± 0.414

1.88 ± 0.24

4.85 ± 0.147

6.531 ± 0.432

8.253 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.654 ± 0.18

4.143 ± 0.331

3.76 ± 0.158

4.243 ± 0.277

5.374 ± 0.214

5.973 ± 0.398

5.466 ± 0.342

6.839 ± 0.366

1.431 ± 0.184

3.469 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski