Morganella morganii subsp. morganii KT
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3510 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7TWS3|J7TWS3_MORMO Ribose-5-phosphate isomerase A OS=Morganella morganii subsp. morganii KT OX=1124991 GN=rpiA PE=3 SV=1
MM1 pKa = 7.81 KK2 pKa = 10.19 IGLFYY7 pKa = 10.96 GSSTCYY13 pKa = 10.49 TEE15 pKa = 3.84 MAAEE19 pKa = 5.11 KK20 pKa = 10.38 IRR22 pKa = 11.84 DD23 pKa = 3.65 ILGDD27 pKa = 4.03 DD28 pKa = 3.77 LVEE31 pKa = 3.93 LHH33 pKa = 6.96 DD34 pKa = 4.49 VKK36 pKa = 10.85 EE37 pKa = 4.33 TDD39 pKa = 3.46 LQLMEE44 pKa = 5.06 SYY46 pKa = 10.29 PVLILGIPTWDD57 pKa = 3.26 FGEE60 pKa = 4.29 IQEE63 pKa = 4.65 DD64 pKa = 3.67 WLAVWEE70 pKa = 4.3 QLPQLDD76 pKa = 3.99 LSGKK80 pKa = 8.4 IVAMYY85 pKa = 11.06 GMGDD89 pKa = 3.35 QVDD92 pKa = 3.87 YY93 pKa = 11.63 SDD95 pKa = 4.19 WFLDD99 pKa = 3.36 ALGYY103 pKa = 10.0 LHH105 pKa = 6.59 QQLQSSGVKK114 pKa = 9.34 FAGYY118 pKa = 9.25 WPVDD122 pKa = 3.5 GYY124 pKa = 9.81 TFTSPKK130 pKa = 10.73 ALTPDD135 pKa = 3.26 GKK137 pKa = 10.21 HH138 pKa = 5.4 FVGLALDD145 pKa = 4.8 DD146 pKa = 3.91 VNQYY150 pKa = 10.98 DD151 pKa = 3.81 EE152 pKa = 4.67 SEE154 pKa = 4.03 EE155 pKa = 5.43 RR156 pKa = 11.84 IATWCMQVLEE166 pKa = 4.66 EE167 pKa = 4.26 VQEE170 pKa = 4.14 LLL172 pKa = 4.65
Molecular weight: 19.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.923
IPC_protein 3.897
Toseland 3.694
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.732
Grimsley 3.605
Solomon 3.872
Lehninger 3.821
Nozaki 3.999
DTASelect 4.202
Thurlkill 3.732
EMBOSS 3.808
Sillero 4.012
Patrickios 1.1
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|M1S524|M1S524_MORMO Soluble pyridine nucleotide transhydrogenase OS=Morganella morganii subsp. morganii KT OX=1124991 GN=sthA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3510
0
3510
1093174
29
3259
311.4
34.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.457 ± 0.054
1.117 ± 0.016
5.45 ± 0.035
5.706 ± 0.04
3.962 ± 0.032
7.344 ± 0.044
2.129 ± 0.025
6.261 ± 0.037
4.579 ± 0.041
10.451 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.74 ± 0.022
3.902 ± 0.033
4.451 ± 0.027
4.212 ± 0.03
5.437 ± 0.036
5.988 ± 0.032
5.657 ± 0.035
6.833 ± 0.039
1.282 ± 0.015
3.04 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here