Amino acid dipepetide frequency for Aminobacter sp. DSM 101952

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.147AlaAla: 17.147 ± 0.182	1.004AlaCys: 1.004 ± 0.031	7.026AlaAsp: 7.026 ± 0.083	7.693AlaGlu: 7.693 ± 0.094	4.726AlaPhe: 4.726 ± 0.072	11.049AlaGly: 11.049 ± 0.087	2.206AlaHis: 2.206 ± 0.035	6.784AlaIle: 6.784 ± 0.075	4.432AlaLys: 4.432 ± 0.067	12.651AlaLeu: 12.651 ± 0.104	3.728AlaMet: 3.728 ± 0.047	3.075AlaAsn: 3.075 ± 0.049	5.163AlaPro: 5.163 ± 0.078	3.548AlaGln: 3.548 ± 0.049	8.358AlaArg: 8.358 ± 0.085	6.721AlaSer: 6.721 ± 0.075	6.111AlaThr: 6.111 ± 0.064	8.903AlaVal: 8.903 ± 0.094	1.482AlaTrp: 1.482 ± 0.034	2.59AlaTyr: 2.59 ± 0.048	0.001AlaXaa: 0.001 ± 0.001
Cys	0.866CysAla: 0.866 ± 0.026	0.103CysCys: 0.103 ± 0.01	0.482CysAsp: 0.482 ± 0.018	0.423CysGlu: 0.423 ± 0.019	0.327CysPhe: 0.327 ± 0.015	0.856CysGly: 0.856 ± 0.03	0.222CysHis: 0.222 ± 0.014	0.379CysIle: 0.379 ± 0.019	0.193CysLys: 0.193 ± 0.011	0.735CysLeu: 0.735 ± 0.025	0.143CysMet: 0.143 ± 0.011	0.205CysAsn: 0.205 ± 0.012	0.414CysPro: 0.414 ± 0.019	0.209CysGln: 0.209 ± 0.011	0.572CysArg: 0.572 ± 0.019	0.446CysSer: 0.446 ± 0.017	0.398CysThr: 0.398 ± 0.016	0.571CysVal: 0.571 ± 0.023	0.118CysTrp: 0.118 ± 0.01	0.204CysTyr: 0.204 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.82AspAla: 6.82 ± 0.079	0.494AspCys: 0.494 ± 0.016	3.264AspAsp: 3.264 ± 0.061	3.583AspGlu: 3.583 ± 0.053	2.288AspPhe: 2.288 ± 0.04	5.361AspGly: 5.361 ± 0.095	1.266AspHis: 1.266 ± 0.031	3.343AspIle: 3.343 ± 0.059	2.076AspLys: 2.076 ± 0.039	5.543AspLeu: 5.543 ± 0.069	1.489AspMet: 1.489 ± 0.032	1.461AspAsn: 1.461 ± 0.034	3.305AspPro: 3.305 ± 0.052	1.691AspGln: 1.691 ± 0.038	4.299AspArg: 4.299 ± 0.068	2.179AspSer: 2.179 ± 0.04	2.554AspThr: 2.554 ± 0.042	4.226AspVal: 4.226 ± 0.056	0.952AspTrp: 0.952 ± 0.024	1.41AspTyr: 1.41 ± 0.032	0.001AspXaa: 0.001 ± 0.001
Glu	7.59GluAla: 7.59 ± 0.08	0.361GluCys: 0.361 ± 0.016	2.742GluAsp: 2.742 ± 0.052	3.078GluGlu: 3.078 ± 0.052	1.992GluPhe: 1.992 ± 0.038	4.292GluGly: 4.292 ± 0.054	1.179GluHis: 1.179 ± 0.028	3.663GluIle: 3.663 ± 0.047	2.523GluLys: 2.523 ± 0.044	5.485GluLeu: 5.485 ± 0.063	1.651GluMet: 1.651 ± 0.037	1.659GluAsn: 1.659 ± 0.035	2.743GluPro: 2.743 ± 0.046	2.128GluGln: 2.128 ± 0.049	4.826GluArg: 4.826 ± 0.062	2.242GluSer: 2.242 ± 0.037	3.462GluThr: 3.462 ± 0.053	3.947GluVal: 3.947 ± 0.051	0.698GluTrp: 0.698 ± 0.022	1.042GluTyr: 1.042 ± 0.027	0.001GluXaa: 0.001 ± 0.001
Phe	4.846PheAla: 4.846 ± 0.071	0.347PheCys: 0.347 ± 0.014	2.753PheAsp: 2.753 ± 0.043	2.334PheGlu: 2.334 ± 0.043	1.539PhePhe: 1.539 ± 0.038	3.922PheGly: 3.922 ± 0.06	0.737PheHis: 0.737 ± 0.024	1.77PheIle: 1.77 ± 0.033	1.078PheLys: 1.078 ± 0.029	3.432PheLeu: 3.432 ± 0.049	0.912PheMet: 0.912 ± 0.026	1.067PheAsn: 1.067 ± 0.027	1.636PhePro: 1.636 ± 0.034	1.09PheGln: 1.09 ± 0.025	2.356PheArg: 2.356 ± 0.038	2.393PheSer: 2.393 ± 0.044	1.918PheThr: 1.918 ± 0.039	3.153PheVal: 3.153 ± 0.053	0.57PheTrp: 0.57 ± 0.022	0.92PheTyr: 0.92 ± 0.026	0.001PheXaa: 0.001 ± 0.001
Gly	9.166GlyAla: 9.166 ± 0.09	0.807GlyCys: 0.807 ± 0.022	4.661GlyAsp: 4.661 ± 0.074	4.932GlyGlu: 4.932 ± 0.069	3.771GlyPhe: 3.771 ± 0.056	7.486GlyGly: 7.486 ± 0.092	1.934GlyHis: 1.934 ± 0.042	4.906GlyIle: 4.906 ± 0.058	3.9GlyLys: 3.9 ± 0.05	8.853GlyLeu: 8.853 ± 0.088	2.517GlyMet: 2.517 ± 0.04	2.345GlyAsn: 2.345 ± 0.052	3.296GlyPro: 3.296 ± 0.047	2.86GlyGln: 2.86 ± 0.045	5.908GlyArg: 5.908 ± 0.071	4.836GlySer: 4.836 ± 0.074	4.658GlyThr: 4.658 ± 0.071	6.106GlyVal: 6.106 ± 0.067	1.384GlyTrp: 1.384 ± 0.031	2.345GlyTyr: 2.345 ± 0.041	0.001GlyXaa: 0.001 ± 0.001
His	2.295HisAla: 2.295 ± 0.047	0.228HisCys: 0.228 ± 0.012	1.29HisAsp: 1.29 ± 0.034	1.072HisGlu: 1.072 ± 0.027	0.907HisPhe: 0.907 ± 0.027	1.967HisGly: 1.967 ± 0.034	0.576HisHis: 0.576 ± 0.025	0.935HisIle: 0.935 ± 0.022	0.545HisLys: 0.545 ± 0.018	1.984HisLeu: 1.984 ± 0.043	0.521HisMet: 0.521 ± 0.017	0.465HisAsn: 0.465 ± 0.018	1.177HisPro: 1.177 ± 0.03	0.619HisGln: 0.619 ± 0.02	1.343HisArg: 1.343 ± 0.033	0.944HisSer: 0.944 ± 0.023	0.742HisThr: 0.742 ± 0.023	1.556HisVal: 1.556 ± 0.034	0.324HisTrp: 0.324 ± 0.015	0.526HisTyr: 0.526 ± 0.017	0.001HisXaa: 0.001 ± 0.001
Ile	7.637IleAla: 7.637 ± 0.081	0.492IleCys: 0.492 ± 0.019	3.802IleAsp: 3.802 ± 0.047	3.679IleGlu: 3.679 ± 0.055	1.824IlePhe: 1.824 ± 0.039	5.16IleGly: 5.16 ± 0.064	0.957IleHis: 0.957 ± 0.024	2.381IleIle: 2.381 ± 0.043	1.615IleLys: 1.615 ± 0.032	4.46IleLeu: 4.46 ± 0.061	1.091IleMet: 1.091 ± 0.026	1.461IleAsn: 1.461 ± 0.034	2.288IlePro: 2.288 ± 0.038	1.155IleGln: 1.155 ± 0.032	3.375IleArg: 3.375 ± 0.047	3.133IleSer: 3.133 ± 0.047	2.549IleThr: 2.549 ± 0.046	4.751IleVal: 4.751 ± 0.061	0.564IleTrp: 0.564 ± 0.022	1.1IleTyr: 1.1 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	4.849LysAla: 4.849 ± 0.066	0.167LysCys: 0.167 ± 0.01	1.897LysAsp: 1.897 ± 0.039	1.717LysGlu: 1.717 ± 0.036	1.02LysPhe: 1.02 ± 0.029	2.91LysGly: 2.91 ± 0.058	0.643LysHis: 0.643 ± 0.022	1.912LysIle: 1.912 ± 0.04	1.594LysLys: 1.594 ± 0.036	3.709LysLeu: 3.709 ± 0.055	0.928LysMet: 0.928 ± 0.027	0.974LysAsn: 0.974 ± 0.028	2.354LysPro: 2.354 ± 0.045	1.104LysGln: 1.104 ± 0.027	2.521LysArg: 2.521 ± 0.044	2.124LysSer: 2.124 ± 0.041	2.121LysThr: 2.121 ± 0.042	2.877LysVal: 2.877 ± 0.048	0.423LysTrp: 0.423 ± 0.017	0.664LysTyr: 0.664 ± 0.023	0.001LysXaa: 0.001 ± 0.001
Leu	13.394LeuAla: 13.394 ± 0.123	0.787LeuCys: 0.787 ± 0.026	6.022LeuAsp: 6.022 ± 0.078	5.199LeuGlu: 5.199 ± 0.061	3.641LeuPhe: 3.641 ± 0.057	8.356LeuGly: 8.356 ± 0.087	1.73LeuHis: 1.73 ± 0.038	4.97LeuIle: 4.97 ± 0.064	3.922LeuLys: 3.922 ± 0.055	9.077LeuLeu: 9.077 ± 0.115	2.348LeuMet: 2.348 ± 0.042	2.508LeuAsn: 2.508 ± 0.044	5.158LeuPro: 5.158 ± 0.058	2.566LeuGln: 2.566 ± 0.044	6.26LeuArg: 6.26 ± 0.07	6.396LeuSer: 6.396 ± 0.065	5.111LeuThr: 5.111 ± 0.063	7.893LeuVal: 7.893 ± 0.076	1.088LeuTrp: 1.088 ± 0.029	2.053LeuTyr: 2.053 ± 0.041	0.001LeuXaa: 0.001 ± 0.001
Met	3.284MetAla: 3.284 ± 0.054	0.154MetCys: 0.154 ± 0.009	1.116MetAsp: 1.116 ± 0.028	1.147MetGlu: 1.147 ± 0.029	0.823MetPhe: 0.823 ± 0.027	1.817MetGly: 1.817 ± 0.035	0.469MetHis: 0.469 ± 0.018	1.352MetIle: 1.352 ± 0.032	1.078MetLys: 1.078 ± 0.026	2.719MetLeu: 2.719 ± 0.048	0.688MetMet: 0.688 ± 0.024	0.846MetAsn: 0.846 ± 0.024	1.554MetPro: 1.554 ± 0.037	0.834MetGln: 0.834 ± 0.026	1.858MetArg: 1.858 ± 0.034	1.752MetSer: 1.752 ± 0.036	1.928MetThr: 1.928 ± 0.036	1.897MetVal: 1.897 ± 0.035	0.224MetTrp: 0.224 ± 0.012	0.342MetTyr: 0.342 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.263AsnAla: 3.263 ± 0.049	0.238AsnCys: 0.238 ± 0.012	1.505AsnAsp: 1.505 ± 0.04	1.354AsnGlu: 1.354 ± 0.035	1.016AsnPhe: 1.016 ± 0.03	2.523AsnGly: 2.523 ± 0.063	0.519AsnHis: 0.519 ± 0.02	1.401AsnIle: 1.401 ± 0.034	0.787AsnLys: 0.787 ± 0.025	2.525AsnLeu: 2.525 ± 0.042	0.665AsnMet: 0.665 ± 0.021	0.69AsnAsn: 0.69 ± 0.03	1.875AsnPro: 1.875 ± 0.035	0.768AsnGln: 0.768 ± 0.021	1.789AsnArg: 1.789 ± 0.037	1.363AsnSer: 1.363 ± 0.035	1.25AsnThr: 1.25 ± 0.029	2.071AsnVal: 2.071 ± 0.044	0.429AsnTrp: 0.429 ± 0.017	0.684AsnTyr: 0.684 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	6.124ProAla: 6.124 ± 0.09	0.273ProCys: 0.273 ± 0.014	3.357ProAsp: 3.357 ± 0.053	3.659ProGlu: 3.659 ± 0.053	1.99ProPhe: 1.99 ± 0.043	4.311ProGly: 4.311 ± 0.062	1.021ProHis: 1.021 ± 0.025	2.292ProIle: 2.292 ± 0.04	1.826ProLys: 1.826 ± 0.037	4.498ProLeu: 4.498 ± 0.063	1.173ProMet: 1.173 ± 0.029	1.348ProAsn: 1.348 ± 0.032	2.072ProPro: 2.072 ± 0.047	1.564ProGln: 1.564 ± 0.036	2.714ProArg: 2.714 ± 0.048	2.646ProSer: 2.646 ± 0.048	2.353ProThr: 2.353 ± 0.038	4.232ProVal: 4.232 ± 0.062	0.637ProTrp: 0.637 ± 0.021	1.194ProTyr: 1.194 ± 0.031	0.001ProXaa: 0.001 ± 0.001
Gln	3.999GlnAla: 3.999 ± 0.056	0.186GlnCys: 0.186 ± 0.013	1.419GlnAsp: 1.419 ± 0.033	1.427GlnGlu: 1.427 ± 0.024	1.097GlnPhe: 1.097 ± 0.03	2.29GlnGly: 2.29 ± 0.042	0.631GlnHis: 0.631 ± 0.018	1.725GlnIle: 1.725 ± 0.033	1.199GlnLys: 1.199 ± 0.031	2.804GlnLeu: 2.804 ± 0.046	0.879GlnMet: 0.879 ± 0.021	0.849GlnAsn: 0.849 ± 0.023	1.723GlnPro: 1.723 ± 0.039	1.153GlnGln: 1.153 ± 0.036	2.304GlnArg: 2.304 ± 0.041	1.689GlnSer: 1.689 ± 0.032	1.543GlnThr: 1.543 ± 0.031	2.2GlnVal: 2.2 ± 0.041	0.389GlnTrp: 0.389 ± 0.015	0.583GlnTyr: 0.583 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	7.175ArgAla: 7.175 ± 0.09	0.478ArgCys: 0.478 ± 0.02	3.942ArgAsp: 3.942 ± 0.058	3.917ArgGlu: 3.917 ± 0.06	2.899ArgPhe: 2.899 ± 0.043	4.683ArgGly: 4.683 ± 0.061	1.671ArgHis: 1.671 ± 0.039	4.034ArgIle: 4.034 ± 0.056	2.648ArgLys: 2.648 ± 0.047	7.599ArgLeu: 7.599 ± 0.072	1.839ArgMet: 1.839 ± 0.037	2.028ArgAsn: 2.028 ± 0.038	3.384ArgPro: 3.384 ± 0.052	2.638ArgGln: 2.638 ± 0.043	5.196ArgArg: 5.196 ± 0.072	3.794ArgSer: 3.794 ± 0.06	3.329ArgThr: 3.329 ± 0.047	4.395ArgVal: 4.395 ± 0.055	0.885ArgTrp: 0.885 ± 0.025	1.629ArgTyr: 1.629 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.366SerAla: 6.366 ± 0.076	0.406SerCys: 0.406 ± 0.018	3.074SerAsp: 3.074 ± 0.045	2.992SerGlu: 2.992 ± 0.044	2.425SerPhe: 2.425 ± 0.042	5.913SerGly: 5.913 ± 0.075	1.149SerHis: 1.149 ± 0.027	2.929SerIle: 2.929 ± 0.055	1.784SerLys: 1.784 ± 0.04	5.489SerLeu: 5.489 ± 0.062	1.38SerMet: 1.38 ± 0.033	1.429SerAsn: 1.429 ± 0.038	2.63SerPro: 2.63 ± 0.038	1.58SerGln: 1.58 ± 0.031	3.708SerArg: 3.708 ± 0.056	3.088SerSer: 3.088 ± 0.051	2.752SerThr: 2.752 ± 0.048	4.157SerVal: 4.157 ± 0.055	0.773SerTrp: 0.773 ± 0.021	1.337SerTyr: 1.337 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	5.995ThrAla: 5.995 ± 0.06	0.405ThrCys: 0.405 ± 0.018	2.784ThrAsp: 2.784 ± 0.047	2.614ThrGlu: 2.614 ± 0.034	2.032ThrPhe: 2.032 ± 0.04	4.995ThrGly: 4.995 ± 0.072	0.967ThrHis: 0.967 ± 0.03	3.023ThrIle: 3.023 ± 0.051	1.583ThrLys: 1.583 ± 0.037	5.471ThrLeu: 5.471 ± 0.062	1.218ThrMet: 1.218 ± 0.029	1.325ThrAsn: 1.325 ± 0.03	2.959ThrPro: 2.959 ± 0.048	1.364ThrGln: 1.364 ± 0.032	3.168ThrArg: 3.168 ± 0.051	2.973ThrSer: 2.973 ± 0.062	2.813ThrThr: 2.813 ± 0.062	4.418ThrVal: 4.418 ± 0.067	0.645ThrTrp: 0.645 ± 0.022	1.169ThrTyr: 1.169 ± 0.035	0.001ThrXaa: 0.001 ± 0.001
Val	9.914ValAla: 9.914 ± 0.094	0.588ValCys: 0.588 ± 0.021	4.378ValAsp: 4.378 ± 0.049	4.742ValGlu: 4.742 ± 0.061	2.954ValPhe: 2.954 ± 0.045	5.816ValGly: 5.816 ± 0.065	1.357ValHis: 1.357 ± 0.031	4.081ValIle: 4.081 ± 0.054	2.517ValLys: 2.517 ± 0.052	7.488ValLeu: 7.488 ± 0.077	1.881ValMet: 1.881 ± 0.036	1.941ValAsn: 1.941 ± 0.039	3.762ValPro: 3.762 ± 0.054	1.938ValGln: 1.938 ± 0.039	4.908ValArg: 4.908 ± 0.063	4.593ValSer: 4.593 ± 0.057	4.483ValThr: 4.483 ± 0.065	6.425ValVal: 6.425 ± 0.072	0.896ValTrp: 0.896 ± 0.025	1.548ValTyr: 1.548 ± 0.033	0.001ValXaa: 0.001 ± 0.001
Trp	1.22TrpAla: 1.22 ± 0.029	0.127TrpCys: 0.127 ± 0.009	0.599TrpAsp: 0.599 ± 0.022	0.523TrpGlu: 0.523 ± 0.021	0.57TrpPhe: 0.57 ± 0.022	0.84TrpGly: 0.84 ± 0.025	0.332TrpHis: 0.332 ± 0.015	0.665TrpIle: 0.665 ± 0.02	0.516TrpLys: 0.516 ± 0.017	1.678TrpLeu: 1.678 ± 0.039	0.374TrpMet: 0.374 ± 0.018	0.459TrpAsn: 0.459 ± 0.017	0.704TrpPro: 0.704 ± 0.022	0.57TrpGln: 0.57 ± 0.021	1.036TrpArg: 1.036 ± 0.029	0.824TrpSer: 0.824 ± 0.025	0.754TrpThr: 0.754 ± 0.024	0.756TrpVal: 0.756 ± 0.023	0.238TrpTrp: 0.238 ± 0.012	0.279TrpTyr: 0.279 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.449TyrAla: 2.449 ± 0.043	0.222TyrCys: 0.222 ± 0.013	1.493TyrAsp: 1.493 ± 0.032	1.216TyrGlu: 1.216 ± 0.03	0.934TyrPhe: 0.934 ± 0.026	2.123TyrGly: 2.123 ± 0.04	0.451TyrHis: 0.451 ± 0.019	0.926TyrIle: 0.926 ± 0.026	0.691TyrLys: 0.691 ± 0.018	2.278TyrLeu: 2.278 ± 0.039	0.457TyrMet: 0.457 ± 0.017	0.582TyrAsn: 0.582 ± 0.02	1.087TyrPro: 1.087 ± 0.032	0.708TyrGln: 0.708 ± 0.022	1.665TyrArg: 1.665 ± 0.036	1.223TyrSer: 1.223 ± 0.03	1.09TyrThr: 1.09 ± 0.029	1.674TyrVal: 1.674 ± 0.038	0.348TyrTrp: 0.348 ± 0.019	0.59TyrTyr: 0.59 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4851 proteins (1488852 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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