Camel associated drosmacovirus 1
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1EKU7|A0A0A1EKU7_9VIRU Uncharacterized protein OS=Camel associated drosmacovirus 1 OX=2169876 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 4.79 IEE4 pKa = 5.05 GLTDD8 pKa = 3.75 PLGVSDD14 pKa = 3.94 TMGAEE19 pKa = 4.08 GGEE22 pKa = 4.21 MVTVSIEE29 pKa = 3.86 EE30 pKa = 4.34 MYY32 pKa = 11.77 DD33 pKa = 3.02 MATSLNKK40 pKa = 9.87 IGIIGIHH47 pKa = 5.67 TPNLGQIARR56 pKa = 11.84 RR57 pKa = 11.84 WAGLMINHH65 pKa = 7.1 KK66 pKa = 8.62 FLRR69 pKa = 11.84 VKK71 pKa = 10.62 SCDD74 pKa = 3.21 VHH76 pKa = 6.4 LACASMLPADD86 pKa = 4.53 PLQIGTTTGTIAPQDD101 pKa = 3.55 MMNPLLYY108 pKa = 10.0 RR109 pKa = 11.84 AVSNEE114 pKa = 3.16 SWNAFTSRR122 pKa = 11.84 LFATSGASVNQNSVKK137 pKa = 10.34 VFTEE141 pKa = 4.13 EE142 pKa = 4.99 FSTQSDD148 pKa = 3.75 EE149 pKa = 4.36 NNEE152 pKa = 4.1 KK153 pKa = 10.05 IYY155 pKa = 11.2 YY156 pKa = 9.13 NALSEE161 pKa = 4.56 PGWKK165 pKa = 9.73 KK166 pKa = 10.69 AFPQSGLDD174 pKa = 3.5 INGLKK179 pKa = 10.18 PLVYY183 pKa = 10.04 PILTQFGNGEE193 pKa = 3.81 AGINNFGLLGSSVQATTPAGNPTTVTNPTTGTAQLDD229 pKa = 3.36 NARR232 pKa = 11.84 YY233 pKa = 9.06 FRR235 pKa = 11.84 GKK237 pKa = 8.9 AQPMPRR243 pKa = 11.84 FPTSYY248 pKa = 9.83 PYY250 pKa = 11.22 VSTDD254 pKa = 2.82 TGSTVGDD261 pKa = 3.27 TWTLMFQDD269 pKa = 4.76 SYY271 pKa = 11.29 QIPRR275 pKa = 11.84 TYY277 pKa = 9.88 VACILTPPGKK287 pKa = 10.2 LIKK290 pKa = 10.22 FYY292 pKa = 10.93 YY293 pKa = 9.61 RR294 pKa = 11.84 LRR296 pKa = 11.84 IVWYY300 pKa = 9.84 IEE302 pKa = 3.7 FSDD305 pKa = 3.59 MCTATEE311 pKa = 3.83 RR312 pKa = 11.84 VVGNSMVTAGNYY324 pKa = 9.8 AYY326 pKa = 10.41 SRR328 pKa = 11.84 QYY330 pKa = 9.91 TIPEE334 pKa = 4.14 AVSSKK339 pKa = 9.5 LTEE342 pKa = 4.71 DD343 pKa = 3.38 STLVTDD349 pKa = 3.95 SDD351 pKa = 4.81 LVSEE355 pKa = 5.33 DD356 pKa = 4.52 DD357 pKa = 4.73 DD358 pKa = 4.71 NSATAVNMVLDD369 pKa = 3.9 KK370 pKa = 11.56 VMEE373 pKa = 4.22 KK374 pKa = 10.84
Molecular weight: 40.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.646
IPC2_protein 4.736
IPC_protein 4.673
Toseland 4.507
ProMoST 4.787
Dawson 4.622
Bjellqvist 4.774
Wikipedia 4.507
Rodwell 4.507
Grimsley 4.418
Solomon 4.622
Lehninger 4.584
Nozaki 4.736
DTASelect 4.914
Thurlkill 4.52
EMBOSS 4.52
Sillero 4.787
Patrickios 3.605
IPC_peptide 4.635
IPC2_peptide 4.774
IPC2.peptide.svr19 4.775
Protein with the highest isoelectric point:
>tr|A0A0A1EL55|A0A0A1EL55_9VIRU Putative replicase protein OS=Camel associated drosmacovirus 1 OX=2169876 GN=rep PE=4 SV=1
MM1 pKa = 7.11 SWSYY5 pKa = 10.86 LYY7 pKa = 9.94 WGQNVARR14 pKa = 11.84 NSPSWTKK21 pKa = 11.18 GNTNQWRR28 pKa = 11.84 YY29 pKa = 10.06 NMSGVPFVGDD39 pKa = 4.24 WIRR42 pKa = 11.84 AADD45 pKa = 3.7 QNQWRR50 pKa = 11.84 QDD52 pKa = 3.31 YY53 pKa = 9.18 MDD55 pKa = 4.0 NYY57 pKa = 9.37 GVGWEE62 pKa = 4.1 DD63 pKa = 3.0 VKK65 pKa = 11.26 YY66 pKa = 10.46 PSMVPGAGSYY76 pKa = 10.53 AHH78 pKa = 7.29 ASTNLIAGSGIVSKK92 pKa = 11.02 NLLGLYY98 pKa = 10.2 RR99 pKa = 11.84 SGRR102 pKa = 11.84 PYY104 pKa = 10.43 KK105 pKa = 10.24 AVPRR109 pKa = 11.84 GGRR112 pKa = 11.84 SVYY115 pKa = 10.56 NRR117 pKa = 3.54
Molecular weight: 13.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.597
IPC2_protein 9.838
IPC_protein 10.262
Toseland 9.692
ProMoST 9.823
Dawson 10.072
Bjellqvist 9.867
Wikipedia 10.35
Rodwell 10.145
Grimsley 10.218
Solomon 10.101
Lehninger 10.043
Nozaki 9.663
DTASelect 9.867
Thurlkill 9.853
EMBOSS 10.145
Sillero 9.97
Patrickios 4.329
IPC_peptide 10.087
IPC2_peptide 8.77
IPC2.peptide.svr19 8.515
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
752
117
374
250.7
28.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.984 ± 0.954
1.064 ± 0.218
5.718 ± 0.339
5.452 ± 1.229
3.191 ± 0.423
7.979 ± 0.722
1.33 ± 0.422
5.053 ± 0.451
5.319 ± 1.328
7.181 ± 0.531
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.59 ± 0.275
5.585 ± 0.735
5.452 ± 0.099
3.457 ± 0.175
4.787 ± 0.807
7.181 ± 1.051
7.314 ± 1.719
6.25 ± 0.517
3.059 ± 1.04
5.053 ± 0.656
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here