Brugia timori

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Brugia

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15861 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R3QKE3|A0A0R3QKE3_9BILA C2H2-type domain-containing protein OS=Brugia timori OX=42155 GN=BTMF_LOCUS6162 PE=4 SV=1
MM1 pKa = 7.44TPIIWLNLLLCIASDD16 pKa = 3.92NEE18 pKa = 4.46TSHH21 pKa = 6.86LPNFGTANEE30 pKa = 4.39VITNNTYY37 pKa = 10.98SGISDD42 pKa = 3.42TFGFLHH48 pKa = 6.13YY49 pKa = 10.99LNFEE53 pKa = 4.33PLFSSEE59 pKa = 4.39FPSDD63 pKa = 3.56PSQLIDD69 pKa = 3.8FAQLSEE75 pKa = 4.46TEE77 pKa = 4.71DD78 pKa = 3.23ISNEE82 pKa = 3.67EE83 pKa = 4.26WKK85 pKa = 10.87SEE87 pKa = 3.94TNQIVNDD94 pKa = 3.91PHH96 pKa = 7.44PGFSDD101 pKa = 3.07IYY103 pKa = 11.3DD104 pKa = 3.95PFGHH108 pKa = 6.55TNLEE112 pKa = 4.0LSLNSDD118 pKa = 2.96MDD120 pKa = 3.73SYY122 pKa = 11.75RR123 pKa = 11.84NLWRR127 pKa = 11.84DD128 pKa = 3.05ANEE131 pKa = 3.95EE132 pKa = 4.34LKK134 pKa = 10.3TGDD137 pKa = 3.68DD138 pKa = 3.59NVRR141 pKa = 11.84YY142 pKa = 9.88ASISDD147 pKa = 3.7AQNEE151 pKa = 4.53TSSSLNLNSDD161 pKa = 4.7DD162 pKa = 4.67IITYY166 pKa = 7.51MM167 pKa = 4.68

Molecular weight: 18.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R3QI57|A0A0R3QI57_9BILA HTH psq-type domain-containing protein OS=Brugia timori OX=42155 GN=BTMF_LOCUS5340 PE=4 SV=1
MM1 pKa = 7.39ALKK4 pKa = 10.48RR5 pKa = 11.84ATGNVKK11 pKa = 9.55TSSPKK16 pKa = 9.86KK17 pKa = 8.51PRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 8.98SAPSGTKK29 pKa = 8.58KK30 pKa = 8.74TANKK34 pKa = 9.69RR35 pKa = 11.84KK36 pKa = 10.21KK37 pKa = 9.62NVKK40 pKa = 9.81KK41 pKa = 10.77SGAKK45 pKa = 8.49RR46 pKa = 11.84TKK48 pKa = 10.57KK49 pKa = 10.2QMKK52 pKa = 9.4KK53 pKa = 10.34

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15861

0

15861

3895967

29

3959

245.6

27.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.053 ± 0.019

2.256 ± 0.016

5.331 ± 0.019

6.495 ± 0.027

4.543 ± 0.016

4.847 ± 0.019

2.426 ± 0.011

6.735 ± 0.02

6.262 ± 0.024

9.565 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.009

5.107 ± 0.017

3.943 ± 0.017

4.042 ± 0.015

5.675 ± 0.017

8.114 ± 0.024

5.47 ± 0.018

6.033 ± 0.016

1.104 ± 0.006

3.256 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski