Feline coronavirus (strain FIPV WSU-79/1146) (FCoV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Tegacovirus; Alphacoronavirus 1; Feline coronavirus; Feline infectious peritonitis virus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O39433|NS3X_FIPV Non-structural protein 3x OS=Feline coronavirus (strain FIPV WSU-79/1146) OX=33734 GN=3x PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 3.83IVKK5 pKa = 10.68SIDD8 pKa = 3.27IFVDD12 pKa = 3.65AVLDD16 pKa = 3.99EE17 pKa = 4.55LDD19 pKa = 3.21RR20 pKa = 11.84AYY22 pKa = 10.64FAVTLKK28 pKa = 11.12VEE30 pKa = 4.99FKK32 pKa = 9.86TGKK35 pKa = 10.29LLVCIGFGDD44 pKa = 3.92TLLEE48 pKa = 4.55AKK50 pKa = 10.4DD51 pKa = 3.28KK52 pKa = 11.16AYY54 pKa = 10.72AKK56 pKa = 10.33LGLSFIEE63 pKa = 4.44EE64 pKa = 4.56VNSHH68 pKa = 4.95TVVV71 pKa = 2.96

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0C545|NS3B_FIPV Putative truncated non-structural protein 3b OS=Feline coronavirus (strain FIPV WSU-79/1146) OX=33734 GN=3b PE=5 SV=1
MM1 pKa = 7.51LSLVSPLLKK10 pKa = 10.35KK11 pKa = 10.86SIVIQLFSITVYY23 pKa = 10.58KK24 pKa = 10.38FKK26 pKa = 11.35AKK28 pKa = 9.99FWYY31 pKa = 9.71KK32 pKa = 10.83LPFEE36 pKa = 4.4TRR38 pKa = 11.84LCIIKK43 pKa = 8.73HH44 pKa = 5.17TRR46 pKa = 11.84PKK48 pKa = 10.43ALSVTKK54 pKa = 9.76QVKK57 pKa = 9.22RR58 pKa = 11.84DD59 pKa = 3.5YY60 pKa = 11.18RR61 pKa = 11.84KK62 pKa = 9.39IAILNSMRR70 pKa = 11.84KK71 pKa = 9.08

Molecular weight: 8.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

1

11

13413

40

6709

1219.4

136.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.345 ± 0.176

3.109 ± 0.239

5.674 ± 0.366

4.6 ± 0.35

5.48 ± 0.214

6.307 ± 0.181

1.841 ± 0.371

5.607 ± 0.383

6.412 ± 0.915

8.701 ± 0.676

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.333

5.838 ± 0.501

3.31 ± 0.113

2.885 ± 0.355

3.101 ± 0.54

6.822 ± 0.384

6.27 ± 0.518

9.603 ± 0.463

1.178 ± 0.163

4.712 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski