Tautonia plasticadhaerens
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7326 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A518HEX4|A0A518HEX4_9BACT Uncharacterized protein OS=Tautonia plasticadhaerens OX=2527974 GN=ElP_73740 PE=4 SV=1
MM1 pKa = 7.17 SQFTHH6 pKa = 6.39 YY7 pKa = 9.45 EE8 pKa = 4.08 HH9 pKa = 7.87 PDD11 pKa = 3.38 PASLEE16 pKa = 4.41 DD17 pKa = 3.66 YY18 pKa = 10.84 CCICGWPLDD27 pKa = 4.03 QPSLRR32 pKa = 11.84 GLAATASPSGSGVGNDD48 pKa = 2.75 WSYY51 pKa = 11.47 FGQLHH56 pKa = 6.54 LPSGLLSIADD66 pKa = 3.82 PCEE69 pKa = 4.42 CSDD72 pKa = 5.73 DD73 pKa = 3.84 GMALIDD79 pKa = 5.63 LMPGDD84 pKa = 4.29 YY85 pKa = 10.71 AITIQTLLLDD95 pKa = 4.07 RR96 pKa = 11.84 ARR98 pKa = 11.84 YY99 pKa = 4.97 TARR102 pKa = 11.84 VRR104 pKa = 11.84 LASEE108 pKa = 4.2 PDD110 pKa = 3.74 GEE112 pKa = 5.03 PGDD115 pKa = 4.81 LLDD118 pKa = 5.71 DD119 pKa = 3.7 VWVDD123 pKa = 3.54 GGQLLLFDD131 pKa = 6.26 DD132 pKa = 5.06 DD133 pKa = 5.44 LGDD136 pKa = 3.91 AAMTTVWTGFGDD148 pKa = 3.25 VRR150 pKa = 11.84 YY151 pKa = 9.24 PIFEE155 pKa = 4.25 VIKK158 pKa = 8.28 EE159 pKa = 3.93 GRR161 pKa = 11.84 RR162 pKa = 11.84 VGVEE166 pKa = 3.38 IAFIDD171 pKa = 3.45 IDD173 pKa = 4.09 FPP175 pKa = 5.38
Molecular weight: 19.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.808
IPC_protein 3.821
Toseland 3.592
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.228
Thurlkill 3.656
EMBOSS 3.795
Sillero 3.948
Patrickios 1.1
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A518HFB3|A0A518HFB3_9BACT Non-specific serine/threonine protein kinase OS=Tautonia plasticadhaerens OX=2527974 GN=pknD_1 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 PGVAARR8 pKa = 11.84 PDD10 pKa = 3.36 PRR12 pKa = 11.84 VARR15 pKa = 11.84 GRR17 pKa = 11.84 DD18 pKa = 3.31 RR19 pKa = 11.84 GPGPGSYY26 pKa = 10.19 ARR28 pKa = 11.84 SSARR32 pKa = 11.84 IRR34 pKa = 11.84 NRR36 pKa = 11.84 RR37 pKa = 11.84 SMPCLSSGRR46 pKa = 11.84 TSSAGDD52 pKa = 3.1 DD53 pKa = 3.17 RR54 pKa = 11.84 VRR56 pKa = 11.84 RR57 pKa = 11.84 EE58 pKa = 3.59 PAGVGRR64 pKa = 11.84 RR65 pKa = 11.84 SASTGWSPGPAEE77 pKa = 4.5 HH78 pKa = 7.01 PHH80 pKa = 5.16 RR81 pKa = 11.84 HH82 pKa = 4.12 QRR84 pKa = 11.84 PRR86 pKa = 11.84 LGAGLAPAGDD96 pKa = 3.38 RR97 pKa = 11.84 RR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 GRR102 pKa = 11.84 RR103 pKa = 11.84 HH104 pKa = 5.75 DD105 pKa = 3.42 RR106 pKa = 11.84 LRR108 pKa = 11.84 RR109 pKa = 11.84 GARR112 pKa = 11.84 RR113 pKa = 11.84 PGHH116 pKa = 5.2 RR117 pKa = 11.84 AGRR120 pKa = 11.84 RR121 pKa = 11.84 RR122 pKa = 11.84 AGPRR126 pKa = 11.84 RR127 pKa = 11.84 GPHH130 pKa = 6.05 DD131 pKa = 3.78 RR132 pKa = 11.84 RR133 pKa = 11.84 PGRR136 pKa = 11.84 RR137 pKa = 11.84 PAGRR141 pKa = 11.84 RR142 pKa = 11.84 RR143 pKa = 11.84 GGPVSGLPGDD153 pKa = 3.88 RR154 pKa = 11.84 GRR156 pKa = 11.84 RR157 pKa = 11.84 GHH159 pKa = 7.06 DD160 pKa = 3.28 RR161 pKa = 11.84 GPGDD165 pKa = 3.36 HH166 pKa = 6.08 QRR168 pKa = 11.84 GRR170 pKa = 11.84 RR171 pKa = 11.84 GRR173 pKa = 11.84 RR174 pKa = 11.84 RR175 pKa = 11.84 DD176 pKa = 3.07 PRR178 pKa = 11.84 RR179 pKa = 11.84 RR180 pKa = 11.84 GRR182 pKa = 11.84 IDD184 pKa = 2.9 ARR186 pKa = 11.84 RR187 pKa = 11.84 FAAGRR192 pKa = 11.84 QPGRR196 pKa = 11.84 RR197 pKa = 11.84 RR198 pKa = 11.84 RR199 pKa = 11.84 ARR201 pKa = 11.84 GRR203 pKa = 11.84 RR204 pKa = 11.84 PGRR207 pKa = 11.84 GRR209 pKa = 11.84 RR210 pKa = 11.84 RR211 pKa = 11.84 HH212 pKa = 4.59 GRR214 pKa = 11.84 GRR216 pKa = 11.84 LAGRR220 pKa = 11.84 RR221 pKa = 11.84 HH222 pKa = 5.27 EE223 pKa = 4.74 HH224 pKa = 7.05 LPLQPGRR231 pKa = 11.84 GRR233 pKa = 11.84 PGPGRR238 pKa = 11.84 RR239 pKa = 11.84 RR240 pKa = 3.36
Molecular weight: 26.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 10.789
IPC_protein 12.384
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.047
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.754
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.191
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7326
0
7326
2572131
29
3546
351.1
38.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.508 ± 0.039
0.884 ± 0.009
6.499 ± 0.024
6.313 ± 0.029
3.247 ± 0.015
9.765 ± 0.044
1.983 ± 0.016
3.962 ± 0.021
1.987 ± 0.021
10.263 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.821 ± 0.012
1.946 ± 0.021
7.046 ± 0.032
2.5 ± 0.017
9.055 ± 0.039
5.561 ± 0.023
4.63 ± 0.028
7.416 ± 0.025
1.506 ± 0.014
2.108 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here