Tortoise microvirus 92

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8WAB6|A0A4P8WAB6_9VIRU Replication initiation protein OS=Tortoise microvirus 92 OX=2583201 PE=4 SV=1
MM1 pKa = 7.34AKK3 pKa = 10.07KK4 pKa = 8.47YY5 pKa = 10.46QKK7 pKa = 10.74YY8 pKa = 9.27EE9 pKa = 4.17EE10 pKa = 4.92PDD12 pKa = 3.61PTPVAVPVGFGHH24 pKa = 8.04PEE26 pKa = 3.66TLEE29 pKa = 3.83QKK31 pKa = 10.0IKK33 pKa = 10.65RR34 pKa = 11.84FVRR37 pKa = 11.84NEE39 pKa = 3.66RR40 pKa = 11.84YY41 pKa = 9.13QAALAAAGAEE51 pKa = 4.22TFEE54 pKa = 4.53EE55 pKa = 4.27SLDD58 pKa = 3.79FNVDD62 pKa = 3.53DD63 pKa = 6.68DD64 pKa = 4.74IDD66 pKa = 3.89PHH68 pKa = 7.12AHH70 pKa = 4.85STPWQEE76 pKa = 3.99RR77 pKa = 11.84ADD79 pKa = 3.85VEE81 pKa = 4.54TEE83 pKa = 3.64IEE85 pKa = 4.21DD86 pKa = 3.65SFRR89 pKa = 11.84AANSHH94 pKa = 6.86AEE96 pKa = 4.17MKK98 pKa = 10.63KK99 pKa = 10.51LEE101 pKa = 4.13ARR103 pKa = 11.84LKK105 pKa = 9.79EE106 pKa = 3.95LQKK109 pKa = 10.36IHH111 pKa = 6.57GPSEE115 pKa = 3.94PSSGGKK121 pKa = 9.17QNGRR125 pKa = 11.84PKK127 pKa = 10.66SKK129 pKa = 9.22STAPAKK135 pKa = 9.61TKK137 pKa = 10.35SGKK140 pKa = 10.17AGSDD144 pKa = 3.23ASDD147 pKa = 4.12DD148 pKa = 3.76ADD150 pKa = 3.28

Molecular weight: 16.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W7W3|A0A4P8W7W3_9VIRU Uncharacterized protein OS=Tortoise microvirus 92 OX=2583201 PE=4 SV=1
MM1 pKa = 7.07EE2 pKa = 4.26TMLRR6 pKa = 11.84PGASTLLGIVCVTPSHH22 pKa = 7.24LLPKK26 pKa = 10.14KK27 pKa = 10.25NRR29 pKa = 11.84ALRR32 pKa = 11.84AQLPSNILVPEE43 pKa = 4.57FDD45 pKa = 3.44SKK47 pKa = 10.62IRR49 pKa = 11.84KK50 pKa = 9.07RR51 pKa = 11.84LPFALDD57 pKa = 3.08ANSARR62 pKa = 11.84KK63 pKa = 9.92SYY65 pKa = 10.3MLLKK69 pKa = 10.61KK70 pKa = 10.47LEE72 pKa = 4.02KK73 pKa = 10.39ALNVNALAYY82 pKa = 10.46QLTQKK87 pKa = 10.68SLAGGKK93 pKa = 8.79MDD95 pKa = 6.67PITAAAVISGGATIIGGLMNRR116 pKa = 11.84DD117 pKa = 3.57SQNSANAQNMEE128 pKa = 4.24INRR131 pKa = 11.84QNAEE135 pKa = 4.01LQKK138 pKa = 10.92EE139 pKa = 4.51FAQNGLRR146 pKa = 11.84WKK148 pKa = 10.91VSDD151 pKa = 3.52AKK153 pKa = 10.99AAGIHH158 pKa = 6.14PLAALGASTTSYY170 pKa = 11.07APAAIGVEE178 pKa = 4.65SNNAMGNAVAGMGQDD193 pKa = 2.81ISRR196 pKa = 11.84AVAATQTANEE206 pKa = 4.11RR207 pKa = 11.84EE208 pKa = 4.16MAEE211 pKa = 3.92MAKK214 pKa = 10.14QSLHH218 pKa = 7.08LDD220 pKa = 3.73LQGKK224 pKa = 9.09SLDD227 pKa = 3.55NAIRR231 pKa = 11.84ASQLAKK237 pKa = 10.89LNATQIGPPMPSAGTKK253 pKa = 9.21PVPSPQNPARR263 pKa = 11.84DD264 pKa = 3.67AGNITSYY271 pKa = 11.77AFMRR275 pKa = 11.84TDD277 pKa = 3.65DD278 pKa = 4.28GGLSVVPSEE287 pKa = 4.14AAKK290 pKa = 10.59EE291 pKa = 3.92RR292 pKa = 11.84TEE294 pKa = 4.75DD295 pKa = 5.02DD296 pKa = 3.86FVQQLGWAARR306 pKa = 11.84NQLVPAVSGLTPPSLKK322 pKa = 10.05EE323 pKa = 3.7HH324 pKa = 7.13PLPKK328 pKa = 9.23GQQWVWNPLKK338 pKa = 10.51QAFYY342 pKa = 10.06PSSGPSAVDD351 pKa = 2.91VYY353 pKa = 11.11NFRR356 pKa = 11.84KK357 pKa = 10.31GGPKK361 pKa = 9.03KK362 pKa = 9.82WKK364 pKa = 10.0IFNDD368 pKa = 3.67KK369 pKa = 10.44YY370 pKa = 11.42

Molecular weight: 39.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1329

150

492

332.3

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.481 ± 1.942

0.978 ± 0.705

5.568 ± 0.731

5.568 ± 1.094

3.386 ± 0.587

7.901 ± 0.53

2.408 ± 0.467

4.665 ± 0.508

6.622 ± 1.136

7.449 ± 0.865

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.339

4.063 ± 0.906

5.944 ± 0.551

3.762 ± 0.728

6.396 ± 0.907

6.546 ± 0.572

6.471 ± 0.978

5.117 ± 0.686

1.354 ± 0.15

3.762 ± 0.864
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski