Sphingobacterium sp. SGG-5

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; unclassified Sphingobacterium

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3361 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M1N7Z1|A0A6M1N7Z1_9SPHI Uncharacterized protein OS=Sphingobacterium sp. SGG-5 OX=2710881 GN=G5B30_07160 PE=4 SV=1
MM1 pKa = 7.68INPNKK6 pKa = 9.75HH7 pKa = 4.58VRR9 pKa = 11.84IALINVLSAATSVPFYY25 pKa = 10.99DD26 pKa = 3.4TMLPTDD32 pKa = 3.81VTPVPSSYY40 pKa = 11.08GIIYY44 pKa = 8.69NQTMSPYY51 pKa = 10.24DD52 pKa = 3.66VSKK55 pKa = 11.42ANYY58 pKa = 9.53DD59 pKa = 3.45WLCGVNIDD67 pKa = 5.32LYY69 pKa = 11.21DD70 pKa = 4.39IGDD73 pKa = 4.04LGSNNSDD80 pKa = 3.78TIDD83 pKa = 4.08DD84 pKa = 4.0MEE86 pKa = 5.68EE87 pKa = 3.94DD88 pKa = 3.7VLNALEE94 pKa = 5.04NGLDD98 pKa = 3.47VNGFIVQEE106 pKa = 4.08VSFLKK111 pKa = 10.77SEE113 pKa = 4.38TVNGQSDD120 pKa = 3.98TNSINRR126 pKa = 11.84KK127 pKa = 8.57VMTYY131 pKa = 8.62EE132 pKa = 3.1IWINRR137 pKa = 11.84VV138 pKa = 2.86

Molecular weight: 15.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M1NAH0|A0A6M1NAH0_9SPHI RsmB/NOP family class I SAM-dependent RNA methyltransferase OS=Sphingobacterium sp. SGG-5 OX=2710881 GN=G5B30_08830 PE=3 SV=1
MM1 pKa = 6.45TTNTAEE7 pKa = 4.02RR8 pKa = 11.84TLILQQNQQHH18 pKa = 6.23RR19 pKa = 11.84SKK21 pKa = 10.51CLVYY25 pKa = 9.3TRR27 pKa = 11.84VMGYY31 pKa = 9.32HH32 pKa = 6.17RR33 pKa = 11.84PVEE36 pKa = 4.17SFNIGKK42 pKa = 9.95KK43 pKa = 9.6GEE45 pKa = 4.04HH46 pKa = 5.71RR47 pKa = 11.84QRR49 pKa = 11.84THH51 pKa = 5.52FSEE54 pKa = 4.46RR55 pKa = 11.84HH56 pKa = 4.25AA57 pKa = 4.49

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3361

0

3361

1259215

38

4303

374.7

42.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.24 ± 0.041

0.71 ± 0.013

5.743 ± 0.026

6.01 ± 0.043

4.73 ± 0.027

7.092 ± 0.042

2.006 ± 0.021

7.039 ± 0.038

6.137 ± 0.045

9.133 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.018

5.301 ± 0.044

3.807 ± 0.027

3.749 ± 0.024

4.499 ± 0.029

6.283 ± 0.031

5.916 ± 0.044

6.695 ± 0.03

1.281 ± 0.016

4.382 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski