Actibacterium atlanticum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Actibacterium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A058ZM99|A0A058ZM99_9RHOB Protein gstA OS=Actibacterium atlanticum OX=1461693 GN=ATO10_08192 PE=4 SV=1
MM1 pKa = 7.29RR2 pKa = 11.84TSTQTGALAAGAWNFEE18 pKa = 4.11IGTQNADD25 pKa = 3.47VLVGSGLRR33 pKa = 11.84DD34 pKa = 3.37LFLGRR39 pKa = 11.84GGDD42 pKa = 3.63DD43 pKa = 3.31RR44 pKa = 11.84LIAGAGNDD52 pKa = 3.59ILIGGHH58 pKa = 6.42GGDD61 pKa = 3.84SYY63 pKa = 11.48EE64 pKa = 4.83ISTTDD69 pKa = 4.03GGTDD73 pKa = 4.48LIWDD77 pKa = 4.16RR78 pKa = 11.84GSAPTGTGYY87 pKa = 11.07YY88 pKa = 9.11ATGEE92 pKa = 4.11DD93 pKa = 3.71RR94 pKa = 11.84VVLDD98 pKa = 4.67GFTSTDD104 pKa = 3.32AALHH108 pKa = 5.98SIGLQISGDD117 pKa = 4.1DD118 pKa = 5.46LILTYY123 pKa = 11.18DD124 pKa = 3.43NAGTTGQIVMRR135 pKa = 11.84DD136 pKa = 4.11FYY138 pKa = 11.34AGNGFFIEE146 pKa = 4.41EE147 pKa = 4.19LDD149 pKa = 3.61IGTGAPAYY157 pKa = 9.92HH158 pKa = 6.63FSYY161 pKa = 10.92LSGDD165 pKa = 2.96NHH167 pKa = 6.65TYY169 pKa = 10.36SVHH172 pKa = 6.56SGPDD176 pKa = 2.96QGGEE180 pKa = 4.11DD181 pKa = 3.08IVVGSNASEE190 pKa = 4.15EE191 pKa = 4.28IYY193 pKa = 10.91AGIGNDD199 pKa = 3.38IIFGGGGADD208 pKa = 2.32TFMFHH213 pKa = 7.76DD214 pKa = 4.28EE215 pKa = 4.22EE216 pKa = 4.47EE217 pKa = 4.69AGGGTDD223 pKa = 4.8IILDD227 pKa = 4.1FDD229 pKa = 4.63PLFDD233 pKa = 6.3AIDD236 pKa = 3.61FTDD239 pKa = 2.85IHH241 pKa = 6.11TLNMSGVAVTEE252 pKa = 3.89NSYY255 pKa = 11.57GNAVITTDD263 pKa = 3.39YY264 pKa = 11.39GVIEE268 pKa = 4.32LAGVSAVDD276 pKa = 3.45VTEE279 pKa = 5.96DD280 pKa = 3.06IFNFAA285 pKa = 4.75

Molecular weight: 29.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A058ZMF8|A0A058ZMF8_9RHOB Phosphomethylpyrimidine kinase OS=Actibacterium atlanticum OX=1461693 GN=ATO10_09148 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3126

0

3126

968251

16

2659

309.7

33.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.845 ± 0.058

0.894 ± 0.014

6.253 ± 0.042

5.983 ± 0.04

3.824 ± 0.028

8.494 ± 0.048

2.058 ± 0.021

5.171 ± 0.03

3.588 ± 0.036

10.071 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.83 ± 0.02

2.77 ± 0.023

5.001 ± 0.031

3.548 ± 0.026

6.116 ± 0.042

5.194 ± 0.031

5.457 ± 0.03

7.259 ± 0.039

1.356 ± 0.018

2.29 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski