Leucobacter sp. Ag1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter; unclassified Leucobacter

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2LFT8|A0A0M2LFT8_9MICO Transcriptional regulator OS=Leucobacter sp. Ag1 OX=1642040 GN=XM48_15885 PE=4 SV=1
MM1 pKa = 7.83TYY3 pKa = 8.84RR4 pKa = 11.84TQAILAQDD12 pKa = 3.99FDD14 pKa = 4.04LQQRR18 pKa = 11.84VQACAATQGVGAVPDD33 pKa = 3.86WAAEE37 pKa = 4.2HH38 pKa = 6.06MWSLSASPGWDD49 pKa = 3.24DD50 pKa = 4.89AYY52 pKa = 11.04ASALEE57 pKa = 4.52AGVEE61 pKa = 4.1APGDD65 pKa = 3.99SEE67 pKa = 5.63AVITDD72 pKa = 3.26AMILAAVQLLATAGGAA88 pKa = 3.4

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2LYL4|A0A0M2LYL4_9MICO Arginase OS=Leucobacter sp. Ag1 OX=1642040 GN=XM48_00980 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.5VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.45GRR40 pKa = 11.84SKK42 pKa = 10.13LTAA45 pKa = 4.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3028

0

3028

961537

28

2397

317.5

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.805 ± 0.069

0.53 ± 0.01

5.801 ± 0.038

6.029 ± 0.044

3.172 ± 0.031

9.283 ± 0.043

1.856 ± 0.021

4.657 ± 0.035

2.131 ± 0.035

10.312 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.749 ± 0.022

1.891 ± 0.023

5.483 ± 0.031

2.806 ± 0.026

7.51 ± 0.062

5.625 ± 0.029

5.722 ± 0.045

8.4 ± 0.041

1.42 ± 0.017

1.817 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski