Amino acid dipepetide frequency for Leucobacter sp. Ag1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.94AlaAla: 20.94 ± 0.245
0.772AlaCys: 0.772 ± 0.033
7.983AlaAsp: 7.983 ± 0.117
9.692AlaGlu: 9.692 ± 0.146
4.0AlaPhe: 4.0 ± 0.062
12.89AlaGly: 12.89 ± 0.139
2.454AlaHis: 2.454 ± 0.056
6.296AlaIle: 6.296 ± 0.095
3.352AlaLys: 3.352 ± 0.079
14.706AlaLeu: 14.706 ± 0.15
2.703AlaMet: 2.703 ± 0.051
2.42AlaAsn: 2.42 ± 0.072
7.05AlaPro: 7.05 ± 0.122
4.112AlaGln: 4.112 ± 0.068
9.316AlaArg: 9.316 ± 0.131
7.331AlaSer: 7.331 ± 0.101
6.722AlaThr: 6.722 ± 0.11
11.25AlaVal: 11.25 ± 0.131
1.775AlaTrp: 1.775 ± 0.046
2.285AlaTyr: 2.285 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.788CysAla: 0.788 ± 0.032
0.057CysCys: 0.057 ± 0.009
0.322CysAsp: 0.322 ± 0.019
0.327CysGlu: 0.327 ± 0.019
0.196CysPhe: 0.196 ± 0.015
0.638CysGly: 0.638 ± 0.029
0.122CysHis: 0.122 ± 0.011
0.188CysIle: 0.188 ± 0.015
0.056CysLys: 0.056 ± 0.008
0.426CysLeu: 0.426 ± 0.025
0.079CysMet: 0.079 ± 0.009
0.107CysAsn: 0.107 ± 0.01
0.248CysPro: 0.248 ± 0.018
0.104CysGln: 0.104 ± 0.011
0.355CysArg: 0.355 ± 0.022
0.324CysSer: 0.324 ± 0.019
0.357CysThr: 0.357 ± 0.02
0.44CysVal: 0.44 ± 0.02
0.075CysTrp: 0.075 ± 0.008
0.096CysTyr: 0.096 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.776AspAla: 8.776 ± 0.115
0.262AspCys: 0.262 ± 0.017
3.307AspAsp: 3.307 ± 0.074
3.909AspGlu: 3.909 ± 0.076
1.77AspPhe: 1.77 ± 0.047
5.957AspGly: 5.957 ± 0.085
1.003AspHis: 1.003 ± 0.034
1.887AspIle: 1.887 ± 0.044
0.854AspLys: 0.854 ± 0.033
5.927AspLeu: 5.927 ± 0.092
0.752AspMet: 0.752 ± 0.028
0.717AspAsn: 0.717 ± 0.03
4.909AspPro: 4.909 ± 0.085
1.437AspGln: 1.437 ± 0.037
5.127AspArg: 5.127 ± 0.091
2.503AspSer: 2.503 ± 0.052
2.577AspThr: 2.577 ± 0.049
4.234AspVal: 4.234 ± 0.071
0.936AspTrp: 0.936 ± 0.029
1.168AspTyr: 1.168 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
7.91GluAla: 7.91 ± 0.112
0.257GluCys: 0.257 ± 0.019
2.645GluAsp: 2.645 ± 0.066
2.982GluGlu: 2.982 ± 0.073
1.934GluPhe: 1.934 ± 0.046
3.872GluGly: 3.872 ± 0.077
1.652GluHis: 1.652 ± 0.045
3.017GluIle: 3.017 ± 0.063
1.309GluLys: 1.309 ± 0.046
7.606GluLeu: 7.606 ± 0.123
0.996GluMet: 0.996 ± 0.035
1.18GluAsn: 1.18 ± 0.036
3.215GluPro: 3.215 ± 0.069
2.675GluGln: 2.675 ± 0.053
6.171GluArg: 6.171 ± 0.106
3.035GluSer: 3.035 ± 0.056
3.232GluThr: 3.232 ± 0.052
4.629GluVal: 4.629 ± 0.086
0.87GluTrp: 0.87 ± 0.031
1.1GluTyr: 1.1 ± 0.034
0.0GluXaa: 0.0 ± 0.0
Phe
4.614PheAla: 4.614 ± 0.077
0.196PheCys: 0.196 ± 0.015
2.305PheAsp: 2.305 ± 0.053
1.908PheGlu: 1.908 ± 0.047
1.118PhePhe: 1.118 ± 0.043
3.634PheGly: 3.634 ± 0.071
0.485PheHis: 0.485 ± 0.019
1.169PheIle: 1.169 ± 0.037
0.485PheLys: 0.485 ± 0.029
2.72PheLeu: 2.72 ± 0.06
0.472PheMet: 0.472 ± 0.024
0.613PheAsn: 0.613 ± 0.028
1.49PhePro: 1.49 ± 0.036
0.758PheGln: 0.758 ± 0.026
1.908PheArg: 1.908 ± 0.045
1.945PheSer: 1.945 ± 0.046
2.01PheThr: 2.01 ± 0.049
2.854PheVal: 2.854 ± 0.061
0.475PheTrp: 0.475 ± 0.023
0.561PheTyr: 0.561 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
11.962GlyAla: 11.962 ± 0.144
0.594GlyCys: 0.594 ± 0.026
5.023GlyAsp: 5.023 ± 0.073
5.234GlyGlu: 5.234 ± 0.084
3.527GlyPhe: 3.527 ± 0.056
8.289GlyGly: 8.289 ± 0.116
1.63GlyHis: 1.63 ± 0.047
5.308GlyIle: 5.308 ± 0.088
2.338GlyLys: 2.338 ± 0.053
9.285GlyLeu: 9.285 ± 0.114
1.85GlyMet: 1.85 ± 0.044
1.685GlyAsn: 1.685 ± 0.039
3.963GlyPro: 3.963 ± 0.065
2.402GlyGln: 2.402 ± 0.054
6.387GlyArg: 6.387 ± 0.088
6.086GlySer: 6.086 ± 0.091
6.081GlyThr: 6.081 ± 0.099
7.589GlyVal: 7.589 ± 0.093
1.557GlyTrp: 1.557 ± 0.041
2.045GlyTyr: 2.045 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
2.272HisAla: 2.272 ± 0.06
0.114HisCys: 0.114 ± 0.011
1.267HisAsp: 1.267 ± 0.041
1.25HisGlu: 1.25 ± 0.039
0.539HisPhe: 0.539 ± 0.022
1.919HisGly: 1.919 ± 0.06
0.464HisHis: 0.464 ± 0.025
0.577HisIle: 0.577 ± 0.025
0.232HisLys: 0.232 ± 0.013
1.947HisLeu: 1.947 ± 0.047
0.271HisMet: 0.271 ± 0.018
0.298HisAsn: 0.298 ± 0.017
1.451HisPro: 1.451 ± 0.037
0.482HisGln: 0.482 ± 0.024
1.697HisArg: 1.697 ± 0.047
0.869HisSer: 0.869 ± 0.034
0.905HisThr: 0.905 ± 0.036
1.317HisVal: 1.317 ± 0.038
0.301HisTrp: 0.301 ± 0.018
0.39HisTyr: 0.39 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.576IleAla: 7.576 ± 0.102
0.287IleCys: 0.287 ± 0.018
3.388IleAsp: 3.388 ± 0.06
3.091IleGlu: 3.091 ± 0.057
1.185IlePhe: 1.185 ± 0.045
4.997IleGly: 4.997 ± 0.082
0.591IleHis: 0.591 ± 0.029
1.773IleIle: 1.773 ± 0.051
0.78IleLys: 0.78 ± 0.032
4.082IleLeu: 4.082 ± 0.074
0.699IleMet: 0.699 ± 0.03
0.825IleAsn: 0.825 ± 0.031
2.605IlePro: 2.605 ± 0.057
0.92IleGln: 0.92 ± 0.033
2.936IleArg: 2.936 ± 0.06
2.458IleSer: 2.458 ± 0.057
2.745IleThr: 2.745 ± 0.059
4.43IleVal: 4.43 ± 0.078
0.526IleTrp: 0.526 ± 0.026
0.673IleTyr: 0.673 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
2.393LysAla: 2.393 ± 0.065
0.083LysCys: 0.083 ± 0.01
1.191LysAsp: 1.191 ± 0.047
0.944LysGlu: 0.944 ± 0.042
0.558LysPhe: 0.558 ± 0.027
1.529LysGly: 1.529 ± 0.049
0.498LysHis: 0.498 ± 0.025
1.074LysIle: 1.074 ± 0.039
0.88LysLys: 0.88 ± 0.044
2.08LysLeu: 2.08 ± 0.058
0.383LysMet: 0.383 ± 0.022
0.65LysAsn: 0.65 ± 0.03
1.411LysPro: 1.411 ± 0.047
0.96LysGln: 0.96 ± 0.035
1.718LysArg: 1.718 ± 0.043
1.241LysSer: 1.241 ± 0.039
1.351LysThr: 1.351 ± 0.048
1.639LysVal: 1.639 ± 0.047
0.279LysTrp: 0.279 ± 0.019
0.45LysTyr: 0.45 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
15.125LeuAla: 15.125 ± 0.174
0.526LeuCys: 0.526 ± 0.027
6.698LeuAsp: 6.698 ± 0.09
5.46LeuGlu: 5.46 ± 0.093
2.838LeuPhe: 2.838 ± 0.063
10.051LeuGly: 10.051 ± 0.121
1.824LeuHis: 1.824 ± 0.048
4.703LeuIle: 4.703 ± 0.082
1.857LeuLys: 1.857 ± 0.059
10.934LeuLeu: 10.934 ± 0.178
1.65LeuMet: 1.65 ± 0.052
1.785LeuAsn: 1.785 ± 0.049
5.77LeuPro: 5.77 ± 0.088
2.478LeuGln: 2.478 ± 0.053
7.899LeuArg: 7.899 ± 0.109
5.864LeuSer: 5.864 ± 0.081
5.867LeuThr: 5.867 ± 0.086
9.132LeuVal: 9.132 ± 0.109
1.233LeuTrp: 1.233 ± 0.042
1.43LeuTyr: 1.43 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
1.775MetAla: 1.775 ± 0.04
0.09MetCys: 0.09 ± 0.01
0.759MetAsp: 0.759 ± 0.028
0.686MetGlu: 0.686 ± 0.028
0.526MetPhe: 0.526 ± 0.026
1.272MetGly: 1.272 ± 0.037
0.352MetHis: 0.352 ± 0.019
0.937MetIle: 0.937 ± 0.039
0.414MetLys: 0.414 ± 0.02
2.037MetLeu: 2.037 ± 0.048
0.283MetMet: 0.283 ± 0.019
0.529MetAsn: 0.529 ± 0.024
1.078MetPro: 1.078 ± 0.037
0.614MetGln: 0.614 ± 0.026
1.483MetArg: 1.483 ± 0.047
1.409MetSer: 1.409 ± 0.037
1.539MetThr: 1.539 ± 0.041
1.267MetVal: 1.267 ± 0.043
0.161MetTrp: 0.161 ± 0.013
0.277MetTyr: 0.277 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.573AsnAla: 2.573 ± 0.075
0.11AsnCys: 0.11 ± 0.011
1.029AsnAsp: 1.029 ± 0.031
0.964AsnGlu: 0.964 ± 0.034
0.567AsnPhe: 0.567 ± 0.025
2.001AsnGly: 2.001 ± 0.058
0.307AsnHis: 0.307 ± 0.017
0.775AsnIle: 0.775 ± 0.03
0.348AsnLys: 0.348 ± 0.021
1.834AsnLeu: 1.834 ± 0.046
0.298AsnMet: 0.298 ± 0.018
0.406AsnAsn: 0.406 ± 0.025
1.535AsnPro: 1.535 ± 0.042
0.519AsnGln: 0.519 ± 0.026
1.347AsnArg: 1.347 ± 0.04
0.936AsnSer: 0.936 ± 0.036
1.159AsnThr: 1.159 ± 0.052
1.467AsnVal: 1.467 ± 0.042
0.306AsnTrp: 0.306 ± 0.019
0.43AsnTyr: 0.43 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.628ProAla: 7.628 ± 0.126
0.213ProCys: 0.213 ± 0.016
3.516ProAsp: 3.516 ± 0.062
4.714ProGlu: 4.714 ± 0.087
1.739ProPhe: 1.739 ± 0.044
5.552ProGly: 5.552 ± 0.088
1.063ProHis: 1.063 ± 0.031
2.258ProIle: 2.258 ± 0.05
1.422ProLys: 1.422 ± 0.043
4.898ProLeu: 4.898 ± 0.084
0.856ProMet: 0.856 ± 0.028
1.185ProAsn: 1.185 ± 0.036
2.078ProPro: 2.078 ± 0.061
1.54ProGln: 1.54 ± 0.043
3.347ProArg: 3.347 ± 0.073
3.11ProSer: 3.11 ± 0.057
3.019ProThr: 3.019 ± 0.065
4.964ProVal: 4.964 ± 0.071
0.766ProTrp: 0.766 ± 0.033
0.965ProTyr: 0.965 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.468GlnAla: 3.468 ± 0.061
0.132GlnCys: 0.132 ± 0.013
1.267GlnAsp: 1.267 ± 0.038
1.3GlnGlu: 1.3 ± 0.043
0.85GlnPhe: 0.85 ± 0.03
2.044GlnGly: 2.044 ± 0.048
0.662GlnHis: 0.662 ± 0.029
1.453GlnIle: 1.453 ± 0.037
0.736GlnLys: 0.736 ± 0.03
3.33GlnLeu: 3.33 ± 0.06
0.525GlnMet: 0.525 ± 0.026
0.681GlnAsn: 0.681 ± 0.027
1.517GlnPro: 1.517 ± 0.052
1.311GlnGln: 1.311 ± 0.048
2.687GlnArg: 2.687 ± 0.057
1.476GlnSer: 1.476 ± 0.039
1.435GlnThr: 1.435 ± 0.044
2.156GlnVal: 2.156 ± 0.049
0.399GlnTrp: 0.399 ± 0.02
0.626GlnTyr: 0.626 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
9.803ArgAla: 9.803 ± 0.13
0.326ArgCys: 0.326 ± 0.018
4.177ArgAsp: 4.177 ± 0.08
4.848ArgGlu: 4.848 ± 0.099
2.725ArgPhe: 2.725 ± 0.058
5.948ArgGly: 5.948 ± 0.086
1.303ArgHis: 1.303 ± 0.047
4.342ArgIle: 4.342 ± 0.065
1.474ArgLys: 1.474 ± 0.041
7.35ArgLeu: 7.35 ± 0.117
1.752ArgMet: 1.752 ± 0.045
1.247ArgAsn: 1.247 ± 0.04
3.683ArgPro: 3.683 ± 0.073
1.802ArgGln: 1.802 ± 0.051
6.315ArgArg: 6.315 ± 0.11
4.48ArgSer: 4.48 ± 0.073
4.562ArgThr: 4.562 ± 0.072
6.312ArgVal: 6.312 ± 0.098
1.147ArgTrp: 1.147 ± 0.038
1.503ArgTyr: 1.503 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
7.59SerAla: 7.59 ± 0.105
0.326SerCys: 0.326 ± 0.019
3.193SerAsp: 3.193 ± 0.058
3.15SerGlu: 3.15 ± 0.063
1.914SerPhe: 1.914 ± 0.054
6.505SerGly: 6.505 ± 0.094
0.93SerHis: 0.93 ± 0.033
2.565SerIle: 2.565 ± 0.053
1.257SerLys: 1.257 ± 0.04
5.186SerLeu: 5.186 ± 0.083
1.139SerMet: 1.139 ± 0.03
1.116SerAsn: 1.116 ± 0.04
2.955SerPro: 2.955 ± 0.053
1.36SerGln: 1.36 ± 0.04
3.76SerArg: 3.76 ± 0.057
3.239SerSer: 3.239 ± 0.074
3.402SerThr: 3.402 ± 0.073
4.549SerVal: 4.549 ± 0.07
0.924SerTrp: 0.924 ± 0.034
1.197SerTyr: 1.197 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
7.886ThrAla: 7.886 ± 0.119
0.281ThrCys: 0.281 ± 0.02
3.084ThrAsp: 3.084 ± 0.066
3.318ThrGlu: 3.318 ± 0.061
1.675ThrPhe: 1.675 ± 0.044
6.072ThrGly: 6.072 ± 0.091
1.049ThrHis: 1.049 ± 0.031
2.695ThrIle: 2.695 ± 0.052
1.255ThrLys: 1.255 ± 0.043
5.557ThrLeu: 5.557 ± 0.088
0.908ThrMet: 0.908 ± 0.032
1.073ThrAsn: 1.073 ± 0.037
3.64ThrPro: 3.64 ± 0.074
1.342ThrGln: 1.342 ± 0.039
3.723ThrArg: 3.723 ± 0.069
3.124ThrSer: 3.124 ± 0.064
3.223ThrThr: 3.223 ± 0.078
5.67ThrVal: 5.67 ± 0.105
0.759ThrTrp: 0.759 ± 0.025
0.885ThrTyr: 0.885 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
10.924ValAla: 10.924 ± 0.128
0.463ValCys: 0.463 ± 0.023
5.034ValAsp: 5.034 ± 0.076
4.84ValGlu: 4.84 ± 0.084
2.798ValPhe: 2.798 ± 0.051
6.865ValGly: 6.865 ± 0.087
1.586ValHis: 1.586 ± 0.043
4.107ValIle: 4.107 ± 0.074
1.632ValLys: 1.632 ± 0.058
9.681ValLeu: 9.681 ± 0.131
1.335ValMet: 1.335 ± 0.045
1.627ValAsn: 1.627 ± 0.043
4.636ValPro: 4.636 ± 0.076
2.2ValGln: 2.2 ± 0.051
6.124ValArg: 6.124 ± 0.09
4.945ValSer: 4.945 ± 0.077
5.11ValThr: 5.11 ± 0.103
7.557ValVal: 7.557 ± 0.104
1.095ValTrp: 1.095 ± 0.032
1.444ValTyr: 1.444 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
1.63TrpAla: 1.63 ± 0.038
0.099TrpCys: 0.099 ± 0.01
0.716TrpAsp: 0.716 ± 0.032
0.629TrpGlu: 0.629 ± 0.029
0.575TrpPhe: 0.575 ± 0.031
1.102TrpGly: 1.102 ± 0.034
0.303TrpHis: 0.303 ± 0.017
0.859TrpIle: 0.859 ± 0.035
0.356TrpLys: 0.356 ± 0.019
1.551TrpLeu: 1.551 ± 0.044
0.292TrpMet: 0.292 ± 0.019
0.424TrpAsn: 0.424 ± 0.023
0.622TrpPro: 0.622 ± 0.029
0.502TrpGln: 0.502 ± 0.025
1.107TrpArg: 1.107 ± 0.041
0.902TrpSer: 0.902 ± 0.031
0.837TrpThr: 0.837 ± 0.038
1.099TrpVal: 1.099 ± 0.032
0.324TrpTrp: 0.324 ± 0.02
0.268TrpTyr: 0.268 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.413TyrAla: 2.413 ± 0.046
0.116TyrCys: 0.116 ± 0.01
1.11TyrAsp: 1.11 ± 0.034
1.04TyrGlu: 1.04 ± 0.037
0.686TyrPhe: 0.686 ± 0.03
1.7TyrGly: 1.7 ± 0.04
0.286TyrHis: 0.286 ± 0.017
0.586TyrIle: 0.586 ± 0.027
0.276TyrLys: 0.276 ± 0.018
1.987TyrLeu: 1.987 ± 0.043
0.243TyrMet: 0.243 ± 0.015
0.342TyrAsn: 0.342 ± 0.02
0.977TyrPro: 0.977 ± 0.036
0.538TyrGln: 0.538 ± 0.024
1.692TyrArg: 1.692 ± 0.043
0.979TyrSer: 0.979 ± 0.034
1.087TyrThr: 1.087 ± 0.043
1.447TyrVal: 1.447 ± 0.046
0.287TyrTrp: 0.287 ± 0.016
0.378TyrTyr: 0.378 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3028 proteins (961537 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski