Equine adenovirus A serotype 1 (EAdV-1) (Equine adenovirus 1)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Rowavirales; Adenoviridae; Mastadenovirus; Equine mastadenovirus A

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G5CZ72|G5CZ72_ADEE1 E1B protein small T-antigen OS=Equine adenovirus A serotype 1 OX=46916 GN=E1Bs PE=3 SV=1
MM1 pKa = 7.88KK2 pKa = 10.32LRR4 pKa = 11.84TMPLCCSLEE13 pKa = 4.04AFVVGLMDD21 pKa = 3.61EE22 pKa = 4.32WQPEE26 pKa = 4.3GLEE29 pKa = 3.92MGYY32 pKa = 9.73SEE34 pKa = 5.42SEE36 pKa = 4.27NEE38 pKa = 4.11SEE40 pKa = 4.29PASLHH45 pKa = 6.63DD46 pKa = 4.72LFDD49 pKa = 3.58IQEE52 pKa = 4.06EE53 pKa = 4.45VLYY56 pKa = 11.16SEE58 pKa = 4.89LEE60 pKa = 4.06NMEE63 pKa = 4.86SEE65 pKa = 4.68LQLTLNTPPVSPVSGGEE82 pKa = 3.97EE83 pKa = 4.11TEE85 pKa = 4.27EE86 pKa = 3.9ASAPPEE92 pKa = 4.23TGSAGAAAAAAPLAEE107 pKa = 4.74SDD109 pKa = 3.83SDD111 pKa = 4.25SEE113 pKa = 4.34GMSEE117 pKa = 4.68GMLRR121 pKa = 11.84CLEE124 pKa = 4.02EE125 pKa = 4.05MPTFDD130 pKa = 5.05EE131 pKa = 4.79DD132 pKa = 4.78DD133 pKa = 3.79EE134 pKa = 5.03VGGGGDD140 pKa = 3.59FEE142 pKa = 4.65TWQTTVPPGSVSGCLRR158 pKa = 11.84CAWYY162 pKa = 10.08QFRR165 pKa = 11.84GEE167 pKa = 4.14ASMCGLCFLKK177 pKa = 10.9ALGGEE182 pKa = 4.07WDD184 pKa = 4.27TVCGG188 pKa = 3.85

Molecular weight: 20.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G5CZ80|G5CZ80_ADEE1 Packaging protein 3 OS=Equine adenovirus A serotype 1 OX=46916 GN=52K PE=2 SV=1
MM1 pKa = 7.56HH2 pKa = 7.46EE3 pKa = 4.01PHH5 pKa = 6.16GHH7 pKa = 6.56LFAGEE12 pKa = 3.97QRR14 pKa = 11.84PHH16 pKa = 6.93VVVKK20 pKa = 10.6RR21 pKa = 11.84RR22 pKa = 11.84LKK24 pKa = 10.42VHH26 pKa = 6.75LLLHH30 pKa = 6.12GLLIRR35 pKa = 11.84GHH37 pKa = 5.17QVRR40 pKa = 11.84GTHH43 pKa = 5.16VGLRR47 pKa = 11.84GADD50 pKa = 3.32AAAGALFEE58 pKa = 4.54VGLRR62 pKa = 11.84EE63 pKa = 4.1HH64 pKa = 6.89PVGGEE69 pKa = 3.9AFAEE73 pKa = 4.22SAPRR77 pKa = 11.84LEE79 pKa = 5.7AEE81 pKa = 4.59MPPALALLSLVVSKK95 pKa = 11.02SILARR100 pKa = 11.84YY101 pKa = 7.67EE102 pKa = 3.95GLGGVPLYY110 pKa = 9.55LHH112 pKa = 6.93PGVPLGRR119 pKa = 11.84GAALEE124 pKa = 4.06SHH126 pKa = 6.25QPLALPLGPLSGGSRR141 pKa = 11.84RR142 pKa = 11.84HH143 pKa = 6.3RR144 pKa = 11.84GLHH147 pKa = 5.65LPQHH151 pKa = 6.28RR152 pKa = 11.84MHH154 pKa = 7.11LRR156 pKa = 11.84RR157 pKa = 11.84STCIPTLCLSVPCRR171 pKa = 11.84TRR173 pKa = 11.84KK174 pKa = 7.26TWPGSPARR182 pKa = 11.84PFIPWSCFGPCEE194 pKa = 3.94TSSIAKK200 pKa = 9.63GSSRR204 pKa = 11.84APAPPRR210 pKa = 11.84PAWRR214 pKa = 11.84GCPSTSTAITASCC227 pKa = 3.86

Molecular weight: 24.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

11012

54

1164

355.2

39.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.499 ± 0.433

1.825 ± 0.329

4.577 ± 0.275

6.003 ± 0.514

4.068 ± 0.304

7.12 ± 0.748

2.624 ± 0.218

3.478 ± 0.188

3.051 ± 0.352

9.481 ± 0.454

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.206

4.023 ± 0.448

6.693 ± 0.347

4.086 ± 0.223

7.646 ± 0.645

7.21 ± 0.358

5.276 ± 0.25

6.856 ± 0.354

1.253 ± 0.121

3.024 ± 0.383
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski