Maritimibacter sp. HL-12
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3527 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X7NV29|A0A1X7NV29_9RHOB Phosphoglucomutase OS=Maritimibacter sp. HL-12 OX=1162418 GN=SAMN05661107_1319 PE=3 SV=1
MM1 pKa = 7.52 LRR3 pKa = 11.84 PALIALALLPAHH15 pKa = 7.08 PALAFDD21 pKa = 4.63 PCDD24 pKa = 3.89 DD25 pKa = 3.72 LWFSRR30 pKa = 11.84 NQLYY34 pKa = 10.78 DD35 pKa = 3.05 RR36 pKa = 11.84 AGYY39 pKa = 10.14 CFSTPLGQAIFDD51 pKa = 4.05 NADD54 pKa = 3.4 CTGTEE59 pKa = 4.05 VTLEE63 pKa = 4.07 PGGTDD68 pKa = 2.98 LVAYY72 pKa = 8.76 VRR74 pKa = 11.84 EE75 pKa = 4.04 MEE77 pKa = 4.25 EE78 pKa = 4.03 RR79 pKa = 11.84 LEE81 pKa = 4.31 CAVDD85 pKa = 3.91 TSATSLDD92 pKa = 3.41 IANIPLRR99 pKa = 11.84 LKK101 pKa = 10.5 LDD103 pKa = 3.62 SVVALSEE110 pKa = 4.15 FASGCLGWTGEE121 pKa = 4.57 AIPLRR126 pKa = 11.84 AGPQDD131 pKa = 3.5 EE132 pKa = 4.91 AEE134 pKa = 4.57 VISVAMPGDD143 pKa = 3.83 DD144 pKa = 5.12 LVWEE148 pKa = 4.39 YY149 pKa = 11.21 EE150 pKa = 4.15 GTNWPEE156 pKa = 3.58 GWSFLTVYY164 pKa = 10.31 RR165 pKa = 11.84 DD166 pKa = 3.41 EE167 pKa = 4.85 AQVSLGFARR176 pKa = 11.84 DD177 pKa = 3.65 DD178 pKa = 4.44 LDD180 pKa = 4.95 YY181 pKa = 11.46 DD182 pKa = 4.08 LCTGLAGG189 pKa = 4.21
Molecular weight: 20.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.126
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.923
Patrickios 1.062
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|A0A1X7PGG8|A0A1X7PGG8_9RHOB Two component transcriptional regulator winged helix family OS=Maritimibacter sp. HL-12 OX=1162418 GN=SAMN05661107_2331 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3527
0
3527
1091098
29
5073
309.4
33.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.298 ± 0.059
0.759 ± 0.014
6.005 ± 0.036
6.236 ± 0.041
3.731 ± 0.03
9.013 ± 0.04
2.097 ± 0.022
5.002 ± 0.032
2.876 ± 0.035
9.994 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.665 ± 0.022
2.379 ± 0.027
5.258 ± 0.036
2.704 ± 0.02
7.104 ± 0.045
4.698 ± 0.03
5.246 ± 0.037
7.413 ± 0.034
1.436 ± 0.02
2.086 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here