Brevundimonas phage vB_BsubS-Delta

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9SV69|A0A6B9SV69_9CAUD Tail terminator OS=Brevundimonas phage vB_BsubS-Delta OX=2696338 GN=DELTA_19 PE=4 SV=1
MM1 pKa = 7.76DD2 pKa = 6.83LIVDD6 pKa = 3.95MLDD9 pKa = 3.92CEE11 pKa = 5.75LIPQVYY17 pKa = 9.84VLEE20 pKa = 5.34SPDD23 pKa = 3.71QPWRR27 pKa = 11.84PVLRR31 pKa = 11.84DD32 pKa = 3.81DD33 pKa = 5.11PYY35 pKa = 11.39EE36 pKa = 4.32PPCLVPGGDD45 pKa = 4.32DD46 pKa = 3.11VWDD49 pKa = 3.58ILL51 pKa = 4.71

Molecular weight: 5.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9SUR9|A0A6B9SUR9_9CAUD Ribonucleoside-diphosphate reductase OS=Brevundimonas phage vB_BsubS-Delta OX=2696338 GN=DELTA_58 PE=3 SV=1
MM1 pKa = 7.19TVQTGTQHH9 pKa = 6.03RR10 pKa = 11.84TGKK13 pKa = 10.1NPTNHH18 pKa = 6.12WRR20 pKa = 11.84PFMEE24 pKa = 4.49VIGRR28 pKa = 11.84GKK30 pKa = 8.71PSYY33 pKa = 9.82MIRR36 pKa = 11.84FHH38 pKa = 6.51LTPFGWWKK46 pKa = 8.85WRR48 pKa = 11.84RR49 pKa = 11.84AYY51 pKa = 10.87LHH53 pKa = 6.36ILGRR57 pKa = 11.84PDD59 pKa = 3.72ADD61 pKa = 3.9RR62 pKa = 11.84EE63 pKa = 4.34YY64 pKa = 10.94HH65 pKa = 5.61DD66 pKa = 5.48HH67 pKa = 7.15PWSFWTLILFGGYY80 pKa = 8.3TEE82 pKa = 4.97TSHH85 pKa = 6.84VMNGRR90 pKa = 11.84GYY92 pKa = 7.82PTGSYY97 pKa = 10.34KK98 pKa = 10.29EE99 pKa = 4.18DD100 pKa = 3.2RR101 pKa = 11.84LGFLSIRR108 pKa = 11.84KK109 pKa = 9.11RR110 pKa = 11.84PATHH114 pKa = 6.24AHH116 pKa = 7.63LITKK120 pKa = 8.86LHH122 pKa = 5.15TRR124 pKa = 11.84RR125 pKa = 11.84VVTLVLRR132 pKa = 11.84DD133 pKa = 3.6NEE135 pKa = 4.41KK136 pKa = 10.29ARR138 pKa = 11.84EE139 pKa = 3.98WGFWSYY145 pKa = 11.31EE146 pKa = 3.9GASCGEE152 pKa = 3.83SGYY155 pKa = 9.87WRR157 pKa = 11.84WIPWKK162 pKa = 9.89VYY164 pKa = 10.57HH165 pKa = 6.34GVNPP169 pKa = 3.99

Molecular weight: 20.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

119

0

119

27025

48

1667

227.1

25.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.008 ± 0.523

0.847 ± 0.099

6.479 ± 0.223

6.468 ± 0.214

3.808 ± 0.158

8.318 ± 0.327

1.858 ± 0.152

4.736 ± 0.14

4.625 ± 0.219

7.586 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.112

3.571 ± 0.142

5.195 ± 0.256

3.597 ± 0.195

6.438 ± 0.233

5.269 ± 0.164

6.043 ± 0.202

6.723 ± 0.193

1.728 ± 0.133

2.994 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski